细枝木麻黄组织培养和转基因研究

[目的]对细枝木麻黄进行组织培养和转基因研究。[方法]以耐寒性细枝木麻黄为试验材料,探讨了转化受体选择压力、菌液浓度、共培养时间在愈伤组织诱导及不定芽分化方面对农杆菌介导转基因转化效率的影响。[结果]对细枝木麻黄不定芽诱导分化适宜的激素组合为DCR+6-BA 5.0 mg/L+NAA 0.5 mg/L;转基因抗生素选择压力为潮霉素,共培养时间3 d;以初步建立的转基因体系利用农杆菌介导法获得94株转基因木麻黄,通过PCR检测,其中61株为PCR阳性植株。[结论]该研究为细枝木麻黄的组织培养和转基因研究奠定了基础。

A Preliminary Study on Tissue Culture and Transformation of Casuarina equisetifolia L.

[Objective] The paper aimed to study the tissue culture and transformation of C. equisetifolia. [Method] C. equisetifolia were used as experimental materials to explore the effects of three conditions including callus induction, adventitious bud dif- ferentiation and Agrobacterium-mediated transformation on transformation rate of C. equisetifolia. [Result] The appropriate plant growth regulator combination on induction and differentiation of C. equisetifolia adventitious buds was DCR＋5.0 mg/L of 6-BA＋ 0.5 mg/L of NAA; hygromycin was selected for the selective pressure and co-culture time was 3 d; 94 stains of transgenic C. equisetifolia were obtained with the initially- established transgene system via Agrobacterium-mediated method, and 61 stains were PCR-positive plants according to the results of PCR detection. [Conclusion] The study had laid the foundation for tissue culture and transgene research of C. equi- setifolia.

“黑金刚三号”火龙果高产栽培技术

火龙果（又名青龙果、红龙果）为仙人掌科三角柱属植物,原产于中美洲热带沙漠地区,属典型的热带植物,火龙果含有美白皮肤的维生素C及丰富的具有减肥、降低血糖、润肠、预防大肠癌的水溶性膳食纤维,是一种低热量、高纤维的水果,除此之外,火龙果在栽培过程中，其果实未见有病虫侵害，是一种无毒、无污染的绿色食品。2000年我镇果农从台湾引进“黑金刚三号”火龙果品种，经过试种取得成功，全镇现种植面积约200多亩。果品深受消费者的喜爱。