[Objective] This study aimed to find methods suitable for the preservation and conidia production of Ustilaginoidea virens. [Method] Six different preservation methods were tested by checking at three month interval f...[Objective] This study aimed to find methods suitable for the preservation and conidia production of Ustilaginoidea virens. [Method] Six different preservation methods were tested by checking at three month interval for the viability and sporulation, and seven liquid media of different composition were tested by shaking cul- ture at 150 r/min. [Result] Among the six methods studied, the periodic transfer and paraffin oil overlay were suitable, and the fungus could survive more than 12 months. The conidia concentration went up to 7.25×10^7 conidia/ml after 9 d in the PSB liquid medium, indicating that PSB was the best medium for conidia production. [Conclusion] This study provides a theoretical basis for the preservation and conidia production of U. virens.展开更多
基金Supported by the Programs of Guizhou Academy of Agricultural Sciences in China [No. QianNongKeYuan(2010) 033 and No.QianNongKeHe 07016]the Programs of Science and Technology Department of Guizhou Province [No.QianKeHe J(2009) 2103,and No.QianKeHe NY(2012) 3031]Science and Technology Project of Guizhou Province [QKH Major Project(2012) 6012]~~
文摘[Objective] This study aimed to find methods suitable for the preservation and conidia production of Ustilaginoidea virens. [Method] Six different preservation methods were tested by checking at three month interval for the viability and sporulation, and seven liquid media of different composition were tested by shaking cul- ture at 150 r/min. [Result] Among the six methods studied, the periodic transfer and paraffin oil overlay were suitable, and the fungus could survive more than 12 months. The conidia concentration went up to 7.25×10^7 conidia/ml after 9 d in the PSB liquid medium, indicating that PSB was the best medium for conidia production. [Conclusion] This study provides a theoretical basis for the preservation and conidia production of U. virens.
