We screened some high-yield fungi of laccase production by bavendamn’s reaction and liquid ferment,and studied the conditions of laccase producing and activities of laccase in them.The results showed that 64 fungi ha...We screened some high-yield fungi of laccase production by bavendamn’s reaction and liquid ferment,and studied the conditions of laccase producing and activities of laccase in them.The results showed that 64 fungi had laccase activities and the Banvendamn’s reaction were positive among the 71 fungi which we tested.The different fungi had different optimal carbon and nitrogen sources in their mediums.For example,Coriolus versicolor’s optimal carbon and nitrogen sources were amylum and casein while Trametes trogii’s were powder of wheatgrass and(NH 4) 2SO 4.The characters of different fungi were different.The optimum temperature and optimum pH of Coriolus versicolor’s laccase reaction were 25℃ and 4.6 respectively,while Trame testrogii’s were 30℃ and 4.0,respectively.K +and Zn 2+ increased activity whereas Ag +obviously inhibited it.展开更多
Activities of the laccase and polyphenol oxidase of Corilus versicolor cultured in PDY fluid medium were studied. The peak of enzyme production appeared in the 9th day for laccase and 14th for polyphenol oxidase in th...Activities of the laccase and polyphenol oxidase of Corilus versicolor cultured in PDY fluid medium were studied. The peak of enzyme production appeared in the 9th day for laccase and 14th for polyphenol oxidase in the constant temperature vibration of 30℃ and 110r/min and the maxmum enzyme activity of the former was 395.6u and the later was 549.0u. The optimum temperature and pH were 25℃ and 4.6 for laccase and 30℃ and 5.0 for polyphenol oxidase. The k + and Zn 2+ were activators for laccase and Mn 2+ ,Ba 2+ , Mg 2+ for polyphenol oxidase, wheras Fe 3+ and Ag + were inhbited for both of them.展开更多
文摘We screened some high-yield fungi of laccase production by bavendamn’s reaction and liquid ferment,and studied the conditions of laccase producing and activities of laccase in them.The results showed that 64 fungi had laccase activities and the Banvendamn’s reaction were positive among the 71 fungi which we tested.The different fungi had different optimal carbon and nitrogen sources in their mediums.For example,Coriolus versicolor’s optimal carbon and nitrogen sources were amylum and casein while Trametes trogii’s were powder of wheatgrass and(NH 4) 2SO 4.The characters of different fungi were different.The optimum temperature and optimum pH of Coriolus versicolor’s laccase reaction were 25℃ and 4.6 respectively,while Trame testrogii’s were 30℃ and 4.0,respectively.K +and Zn 2+ increased activity whereas Ag +obviously inhibited it.
文摘Activities of the laccase and polyphenol oxidase of Corilus versicolor cultured in PDY fluid medium were studied. The peak of enzyme production appeared in the 9th day for laccase and 14th for polyphenol oxidase in the constant temperature vibration of 30℃ and 110r/min and the maxmum enzyme activity of the former was 395.6u and the later was 549.0u. The optimum temperature and pH were 25℃ and 4.6 for laccase and 30℃ and 5.0 for polyphenol oxidase. The k + and Zn 2+ were activators for laccase and Mn 2+ ,Ba 2+ , Mg 2+ for polyphenol oxidase, wheras Fe 3+ and Ag + were inhbited for both of them.
