高效疏水作用色谱填料的合成及其在重组人干扰素纯化中的应用

The synthesis of packing of oxygen-ethane as end-ligand bonded to silica andseparation for recombinant human interferons(rhIFN) in high-performance hydrophobicinteraction chromatography has been studied in this paper. It was showed that the packingsynthesized was not only suitable for separating standard proteins, but also for different rhIFNs,i. e., rhIFN-αA expressed in yeast, rhIFN-αA and rh1FN-γ in E. colt. The purify of threerhIFNs purified by one-step HPHIC, which rhIFN-γ expressed in E. colt was recognized as80%, rhIFN-αA expressed in yeast as 70%, and rhIFN-αA expressed in E. colt as 30%, wasmore than that of other chromatography besides affinity chromatography.

通气颗粒对小鼠胃肠功能的影响

目的:验证通气颗粒的主要功效。方法:采用小鼠胃酚红排空法,正常和脾虚小鼠炭末推进法,醋酸扭体法。结果:通气颗粒中、高剂量组(4,8g·kg^1)灌胃给药可显著促进正常小鼠胃酚红排空运动(P<0.01);通气颗粒中、高2个剂量组(4,8g·kg^1)灌胃给药可显著促进正常小鼠小肠推动功能(P<0.05,P<0.01);通气颗粒低、中、高3个剂量组(2,4,8g·kg^1)连续灌胃给药5d,可显著促进牌虚小鼠小肠推进功能(P<0.05,P<0.01);通气颗粒中、高剂量组(4,8g·kg^1)连续灌胃给药3d对醋酸模型小鼠有显著的镇痛作用(P<0.01)结论:通气颗粒可促进胃肠蠕动。

影响生物组织图像灰度测量的因素探讨

本文在ＭＩＡＳ－３００型图象分析仪上对影响生物图象灰度测量的因素进行了探讨，发现显微光照强度、增益校正值（Ｇａｉｎ）和半灰度门限分割值等（Ｔ２）均可影响灰度测量结果，并直接影响到生物样品化学成份的定量分析，这些因素应引起测试者的高度重视。