2种植物内生固氮菌对燕麦种子萌发及幼苗生长的效果

为探明不同来源内生固氮菌对燕麦植株的促生能力,为植物内生固氮菌的应用开发提供参考,以陇燕3号接种沙蓬内生固氮菌产酸克雷伯氏菌(Klepsiella oxytoca Msp2c)和苜蓿内生固氮菌荧光假单胞杆菌(Pseudomonas fluorescence LM531)菌液,研究2种植物内生固氮菌对燕麦种子萌发及幼苗生长的效果。结果表明:接种产酸克雷伯氏菌菌液的燕麦出苗率、发芽率、株高、根长、根体积、叶面积、地上部鲜重、地下部鲜重均最高,分别达93.67%、89.00%、284.3 mm、242.3 mm、0.5 cm3、663.8 mm2、0.3998 g和0.3058 g,均显著高于无菌水处理对照组和接种荧光假单胞杆菌处理组。荧光假单胞杆菌处理组的株高、根长低于对照组,除出苗率高于对照组外其余指标均低于对照组且差异不显著。产酸克雷伯氏菌对燕麦幼苗有显著促生作用,可用于沙漠地区燕麦固氮促生菌剂的研制。

马昆德拟青霉菌株产维生素A和维生素E的优化

为进一步提高一株马昆德拟青霉(Paecilomyces marquandii)菌株维生素A和维生素E的产量,以PDA液体培养基为基础培养基,采用正交试验方法,比较了添加氮源、pH及Fe2+添加对菌株产维生素A及维生素E的影响。结果表明,添加氮源为硝酸钠,pH为6,不添加Fe2+,菌株维生素A含量明显提高;添加氮源为蛋白胨,有助于提高菌丝维生素E含量。

Optimization of V_A and V_E Production by a Paecilomyces marquandii Strain

In order to further improve the yield of VA and VE produced by a Paecilomyces marquandii strain, using PDA liquid medium as the basic medium, the effects of adding nitrogen source, pH value and Fe2＋ on the VA and VE production were compared with an orthogonal experiment. The results showed that the VA yield was significantly increased when sodium nitrate was added as nitrogen source, pH value of medium was 6, but Fe2＋ was not added; the addition of peptone into the basic medium as a nitrogen source contributed to the improvement of VE yield in mycelia; the PDA liquid medium, as a basic medium, was more conducive to the VA production by the Paecilomyces marquandii strain.