为有效地预防烟草黑胫病在毕节市的流行与发生,在2010-2012年对毕节市烟草黑胫病在不同品种和海拔高度的发生情况进行了调查,并应用形态学方法、分子试验和回接实验对病原菌进行了分类鉴定。结果表明:不同海拔烤烟种植区烟草黑胫病的发...为有效地预防烟草黑胫病在毕节市的流行与发生,在2010-2012年对毕节市烟草黑胫病在不同品种和海拔高度的发生情况进行了调查,并应用形态学方法、分子试验和回接实验对病原菌进行了分类鉴定。结果表明:不同海拔烤烟种植区烟草黑胫病的发病情况差异较大,以毕节市东部地区的黑胫病发生较为普遍,而海拔较高的西部地区发病较少;不同烟草栽培品种对黑胫病的抗性差异较大,感病程度为云烟87>毕纳1号>云烟97;此外,已分离的病原菌鉴定结果为烟草疫霉烟草变种(Phytophthora nicotianae var. nicotianae)。展开更多
In order to explore the fertilizing ways and dynamic changes of soil carbon and nitrogen in the main producing areas of tobacco in Guizhou,research was conducted to study the variations of dissolved organic carbon and...In order to explore the fertilizing ways and dynamic changes of soil carbon and nitrogen in the main producing areas of tobacco in Guizhou,research was conducted to study the variations of dissolved organic carbon and nitrogen,total organic carbon and nitrogen and their ratio of tobacco-topsoil in organic fertilization pattern and conventional cultivation pattern (No fertilizer as control) by pot experiment.The results were as follows:(1) The effects of different fertilization patterns on soil dissolved organic carbon and nitrogen and total organic carbon and nitrogen were significantly different.The content of DOC,DON,TOC and TON in tobaccotopsoil was decreased with the advancing of growth period in conventional fertilization pattern.In the conventional fertilization pattern,the accumulation of DOC and TOC was increased first and then decreased,and the accumulation of DON and TON was decreased first and then increased.(2) The TOC content at the different growing stage and DOC content at the middle and later stage of tobacco were significantly improved in organic fertilization patterns.The accumulation of DON and TON in the conventional fertilization pattern was significantly higher than those in the organic fertilizer pattern and control at the rosette stage and vigorous stage.In the harvest period,the content of DOC,DON,TOC and TON of tobacco-topsoil in the conventional fertilization and organic fertilization pattern was significantly higher than those in the control.(3)The DOC/DON ratio and the TOC/TiON ratio was increased gradually with the advancing of growth period in conventional fertilization pattern,but they were increased first and then decreased in the organic fertilization pattern and CK.The DOC/DON and TOC/TON ratio of tobacco-topsoil in different fertilization patterns was showed as Y J> CK> CG at the different growing stage.The experiment results revealed that:The organic fertilization pattern may improve significantly the accumulation of DOC,DON,TOC and TON of tobacco-topsoil at the middle and later stage and the DOC/DON and TOC/TON ratio at the different growth stage.It contributed to the continuous and balanced supply of nutrients at the middle and later stage of tobacco and the soil fertility.展开更多
文摘为有效地预防烟草黑胫病在毕节市的流行与发生,在2010-2012年对毕节市烟草黑胫病在不同品种和海拔高度的发生情况进行了调查,并应用形态学方法、分子试验和回接实验对病原菌进行了分类鉴定。结果表明:不同海拔烤烟种植区烟草黑胫病的发病情况差异较大,以毕节市东部地区的黑胫病发生较为普遍,而海拔较高的西部地区发病较少;不同烟草栽培品种对黑胫病的抗性差异较大,感病程度为云烟87>毕纳1号>云烟97;此外,已分离的病原菌鉴定结果为烟草疫霉烟草变种(Phytophthora nicotianae var. nicotianae)。
基金Supported by The General Program of National Natural Science Foundation of China(312771673)Programs for Science and Technology Development of Tobacco Monopoly Bureau in Guizhou Province(20121126)~~
文摘In order to explore the fertilizing ways and dynamic changes of soil carbon and nitrogen in the main producing areas of tobacco in Guizhou,research was conducted to study the variations of dissolved organic carbon and nitrogen,total organic carbon and nitrogen and their ratio of tobacco-topsoil in organic fertilization pattern and conventional cultivation pattern (No fertilizer as control) by pot experiment.The results were as follows:(1) The effects of different fertilization patterns on soil dissolved organic carbon and nitrogen and total organic carbon and nitrogen were significantly different.The content of DOC,DON,TOC and TON in tobaccotopsoil was decreased with the advancing of growth period in conventional fertilization pattern.In the conventional fertilization pattern,the accumulation of DOC and TOC was increased first and then decreased,and the accumulation of DON and TON was decreased first and then increased.(2) The TOC content at the different growing stage and DOC content at the middle and later stage of tobacco were significantly improved in organic fertilization patterns.The accumulation of DON and TON in the conventional fertilization pattern was significantly higher than those in the organic fertilizer pattern and control at the rosette stage and vigorous stage.In the harvest period,the content of DOC,DON,TOC and TON of tobacco-topsoil in the conventional fertilization and organic fertilization pattern was significantly higher than those in the control.(3)The DOC/DON ratio and the TOC/TiON ratio was increased gradually with the advancing of growth period in conventional fertilization pattern,but they were increased first and then decreased in the organic fertilization pattern and CK.The DOC/DON and TOC/TON ratio of tobacco-topsoil in different fertilization patterns was showed as Y J> CK> CG at the different growing stage.The experiment results revealed that:The organic fertilization pattern may improve significantly the accumulation of DOC,DON,TOC and TON of tobacco-topsoil at the middle and later stage and the DOC/DON and TOC/TON ratio at the different growth stage.It contributed to the continuous and balanced supply of nutrients at the middle and later stage of tobacco and the soil fertility.