[Objective] This study was to explore the mutagenic effect of 60Co γ-ray on Solanum tuberosum chromosomes.[Method] The 60Co γ-ray at different irradiation doses was applied to treat the minitubers of potato variety ...[Objective] This study was to explore the mutagenic effect of 60Co γ-ray on Solanum tuberosum chromosomes.[Method] The 60Co γ-ray at different irradiation doses was applied to treat the minitubers of potato variety ＇Favorita＇,and the genomic mutations in VM1 and VM2 gene rations in treatments with different doses were analyzed by SRAP markers.[Resalt] Thirty pairs of SRAP primers out of 88 pairs exhibited polymorphism with a rate of 34.1％ in the bulked selection of VM1 generation.A total of 225 bands were obtained,of which 64 were polymorphic with a rate of 28.4％.The polymorphism was reflected in the forms of deleted bands and added bands.Based on the result of the bulked selection of VM1 generation,25 primers with polymorphism were selected to scan VM2 generation.Five primers performed poorly,and nine of the rest 20 pairs revealed polymorphism and obtained nine polymorphic bands,of which only four bands were detected in VM1 generation and the other five ones were newly deleted bands.Only 9.8％ of the bands detected in VM1 generation were obtained in VM2 generation.Eventually,nine stable and dear polymorphic bands were recovered and cloned,and DNA sequences of six bands of them were acquired by sequencing.According to the comparative analysis,five fragments sequences were similar to potato chromosome with a similarity rate of 77％-89％,three of them located at the resistance gene cluster; another one fragment had a similarity of 93％ with some regions of the No.5 chromosome in tomato.[Conclusion] 60Co γ-ray irradiation can cause mutation of genomic DNA in potato;there is no significant correlation between the number of polymorphic bands and the irradiation dose; potymorphic bands are characterized by a larger number of deleted bands than that of the added ones.展开更多
文摘[Objective] This study was to explore the mutagenic effect of 60Co γ-ray on Solanum tuberosum chromosomes.[Method] The 60Co γ-ray at different irradiation doses was applied to treat the minitubers of potato variety ＇Favorita＇,and the genomic mutations in VM1 and VM2 gene rations in treatments with different doses were analyzed by SRAP markers.[Resalt] Thirty pairs of SRAP primers out of 88 pairs exhibited polymorphism with a rate of 34.1％ in the bulked selection of VM1 generation.A total of 225 bands were obtained,of which 64 were polymorphic with a rate of 28.4％.The polymorphism was reflected in the forms of deleted bands and added bands.Based on the result of the bulked selection of VM1 generation,25 primers with polymorphism were selected to scan VM2 generation.Five primers performed poorly,and nine of the rest 20 pairs revealed polymorphism and obtained nine polymorphic bands,of which only four bands were detected in VM1 generation and the other five ones were newly deleted bands.Only 9.8％ of the bands detected in VM1 generation were obtained in VM2 generation.Eventually,nine stable and dear polymorphic bands were recovered and cloned,and DNA sequences of six bands of them were acquired by sequencing.According to the comparative analysis,five fragments sequences were similar to potato chromosome with a similarity rate of 77％-89％,three of them located at the resistance gene cluster; another one fragment had a similarity of 93％ with some regions of the No.5 chromosome in tomato.[Conclusion] 60Co γ-ray irradiation can cause mutation of genomic DNA in potato;there is no significant correlation between the number of polymorphic bands and the irradiation dose; potymorphic bands are characterized by a larger number of deleted bands than that of the added ones.
