AIM:To establish pupil diameter measurement algorithms based on infrared images that can be used in real-world clinical settings.METHODS:A total of 188 patients from outpatient clinic at He Eye Specialist Shenyang Hos...AIM:To establish pupil diameter measurement algorithms based on infrared images that can be used in real-world clinical settings.METHODS:A total of 188 patients from outpatient clinic at He Eye Specialist Shenyang Hospital from Spetember to December 2022 were included,and 13470 infrared pupil images were collected for the study.All infrared images for pupil segmentation were labeled using the Labelme software.The computation of pupil diameter is divided into four steps:image pre-processing,pupil identification and localization,pupil segmentation,and diameter calculation.Two major models are used in the computation process:the modified YoloV3 and Deeplabv 3+models,which must be trained beforehand.RESULTS:The test dataset included 1348 infrared pupil images.On the test dataset,the modified YoloV3 model had a detection rate of 99.98% and an average precision(AP)of 0.80 for pupils.The DeeplabV3+model achieved a background intersection over union(IOU)of 99.23%,a pupil IOU of 93.81%,and a mean IOU of 96.52%.The pupil diameters in the test dataset ranged from 20 to 56 pixels,with a mean of 36.06±6.85 pixels.The absolute error in pupil diameters between predicted and actual values ranged from 0 to 7 pixels,with a mean absolute error(MAE)of 1.06±0.96 pixels.CONCLUSION:This study successfully demonstrates a robust infrared image-based pupil diameter measurement algorithm,proven to be highly accurate and reliable for clinical application.展开更多
目的探讨桑白皮不同有效部位群对小鼠呼吸道合胞病毒肺炎病毒载量的影响。方法小鼠按体质量等级随机分为5组,分别为正常组、模型组、桑白皮总黄酮组、桑白皮总多糖组、病毒唑组,每组30只。以呼吸道合胞病毒滴鼻感染小鼠,治疗组分别予桑...目的探讨桑白皮不同有效部位群对小鼠呼吸道合胞病毒肺炎病毒载量的影响。方法小鼠按体质量等级随机分为5组,分别为正常组、模型组、桑白皮总黄酮组、桑白皮总多糖组、病毒唑组,每组30只。以呼吸道合胞病毒滴鼻感染小鼠,治疗组分别予桑白皮总黄酮、桑白皮总多糖、病毒唑干预。每日观察小鼠的生存状态;呼吸道合胞病毒感染后3、5、7 d计算肺指数,实时荧光定量PCR(real time RT-PCR)检测小鼠肺组织中病毒载量。结果模型组与正常组比较肺指数在不同时间点有不同程度的增高(P<0.05);各治疗组与模型组比较,第3、5、7天肺指数均明显降低(P<0.05),总黄酮及总多糖组明显优于病毒唑组(P<0.05)。正常组肺组织未检测到呼吸道合胞病毒,模型组病毒载量随时间逐渐增多。各治疗组与模型组相比各时间点病毒载量有不同程度降低,但桑白皮总黄酮组最低,与桑白皮总多糖及病毒唑组比较差异有统计学意义(P<0.05)。结论桑白皮总黄酮对呼吸道合胞病毒肺炎有一定的治疗作用,优于桑白皮总多糖及病毒唑组。展开更多
文摘AIM:To establish pupil diameter measurement algorithms based on infrared images that can be used in real-world clinical settings.METHODS:A total of 188 patients from outpatient clinic at He Eye Specialist Shenyang Hospital from Spetember to December 2022 were included,and 13470 infrared pupil images were collected for the study.All infrared images for pupil segmentation were labeled using the Labelme software.The computation of pupil diameter is divided into four steps:image pre-processing,pupil identification and localization,pupil segmentation,and diameter calculation.Two major models are used in the computation process:the modified YoloV3 and Deeplabv 3+models,which must be trained beforehand.RESULTS:The test dataset included 1348 infrared pupil images.On the test dataset,the modified YoloV3 model had a detection rate of 99.98% and an average precision(AP)of 0.80 for pupils.The DeeplabV3+model achieved a background intersection over union(IOU)of 99.23%,a pupil IOU of 93.81%,and a mean IOU of 96.52%.The pupil diameters in the test dataset ranged from 20 to 56 pixels,with a mean of 36.06±6.85 pixels.The absolute error in pupil diameters between predicted and actual values ranged from 0 to 7 pixels,with a mean absolute error(MAE)of 1.06±0.96 pixels.CONCLUSION:This study successfully demonstrates a robust infrared image-based pupil diameter measurement algorithm,proven to be highly accurate and reliable for clinical application.
文摘目的探讨桑白皮不同有效部位群对小鼠呼吸道合胞病毒肺炎病毒载量的影响。方法小鼠按体质量等级随机分为5组,分别为正常组、模型组、桑白皮总黄酮组、桑白皮总多糖组、病毒唑组,每组30只。以呼吸道合胞病毒滴鼻感染小鼠,治疗组分别予桑白皮总黄酮、桑白皮总多糖、病毒唑干预。每日观察小鼠的生存状态;呼吸道合胞病毒感染后3、5、7 d计算肺指数,实时荧光定量PCR(real time RT-PCR)检测小鼠肺组织中病毒载量。结果模型组与正常组比较肺指数在不同时间点有不同程度的增高(P<0.05);各治疗组与模型组比较,第3、5、7天肺指数均明显降低(P<0.05),总黄酮及总多糖组明显优于病毒唑组(P<0.05)。正常组肺组织未检测到呼吸道合胞病毒,模型组病毒载量随时间逐渐增多。各治疗组与模型组相比各时间点病毒载量有不同程度降低,但桑白皮总黄酮组最低,与桑白皮总多糖及病毒唑组比较差异有统计学意义(P<0.05)。结论桑白皮总黄酮对呼吸道合胞病毒肺炎有一定的治疗作用,优于桑白皮总多糖及病毒唑组。
