栽培模式对龙牙百合种植地土壤微生物多样性的影响

为了降低龙牙百合栽培过程中农药的使用量,提高龙牙百合的产量和品质,设置空白地、地膜覆盖+一次性施肥种植模式(PM+OTF)与传统露地栽培模式(OFC)3个处理,对不同处理土壤中的细菌、真菌多样性进行比较,分析种植模式的改变对龙牙百合地下部生长微生物环境的影响。结果表明,PM+OTF模式的土壤真菌群落丰富度较OFC模式低,但群落多样性不存在显著差异;2种栽培模式土壤的细菌丰富度及多样性不存在显著差异。PCA主成分分析表明,PM+OTF模式的土壤微生物群落结构组成更接近空白地。土壤真菌与细菌群落组成分析显示,2种栽培模式在门水平上的微生物多样性不存在显著性差异;但细菌的被孢霉属(Aquisphaera)、芽孢杆菌属(Bacillus)、嗜酸栖热菌属(Acidothermus)等存在显著差异(P<0.05),真菌的珊瑚菌属(Clavaria)、踝节菌属(Talaromyces)、莫蒂埃菌属(Mortierella)、绿核菌属(Ustilaginoidea)、镰刀菌属(Fusarium)、青霉属(Penicillium)等存在显著差异(P<0.05)。从产量来看,与OFC模式相比,PM+OTF模式增产10.5%,优品率提高31.9%,叶面病害显著降低。综合分析,地膜覆盖+一次性施肥种植模式具有控制和减轻龙牙百合病害发生的潜力。

基于叶绿体DNA条形码的竹子种属聚类分析与耐盐性鉴定

利用3种叶绿体DNA条形码序列ndhF、psbA–trnH、rps16对竹亚科植物17个属的98个竹种进行DNA条形码聚类分析,并对已知的43个耐盐竹种进行聚类分析。结果显示:3种DNA条形码序列对竹子具有良好的通用性,平均通用性在96%以上;psbA–trnH未能对瓜多竹外的竹亚科植物种属进行聚类,能对部分耐盐竹种进行聚类;ndhF、rps16条形码序列能对部分种属进行聚类,对牡竹属、箣竹属、苦竹属中包含的耐盐竹种聚类效果较好。

Bioinformatics Analysis of Upland Cotton Gene GhCRE1

Cytokinin plays a very important role in plants growth and development,and CRE1 has been identified as a cytokinin receptor.However,the biological function of CRE1 in cotton remains unclear.In this paper,GhCRE1 gene was cloned and its sequence was analyzed by bioinformatics.The results revealed that GhCRE1 had 11 exons and 10 introns,and its molecular weight,theoretical isoelectric point(pI)and number of amino acids differed from those of the homologous gene in Theobroma cacao and Arabidopsis thaliana,with three different protein domains.15 unidentified proteins were found in potential interaction with CRE1 protein and 2 phosphorylation sites on CRE1 protein sequence were predicted by mutiple bioinformatics websites.Additionally,8 cis-acting regulatory elements were detected on CRE1 promoter sequence,and found related to light signal and hormone.These results were conducive to unfolding the function of GhCRE1 in upland cotton.