探讨在本科临床实习医生中应用以问题为基础的学习(Problem Based Learning,PBL)教学法与情景模拟示教相结合,评估其手卫生执行率和正确率的效果。通过这种方式,我们希望能够改善手卫生教育干预的应用效果,提高临床实习医生的手卫生水平...探讨在本科临床实习医生中应用以问题为基础的学习(Problem Based Learning,PBL)教学法与情景模拟示教相结合,评估其手卫生执行率和正确率的效果。通过这种方式,我们希望能够改善手卫生教育干预的应用效果,提高临床实习医生的手卫生水平,降低医院感染发生率。方法 选取2020年至2021年在重庆医科大学附属南川医院神经内科轮转的本科临床实习生68人为教学研究对象,按随机数字表法分成观察组(34人)和对照组(34人),对照组实习生接受传统的情景模拟示教教学模式,观察组实习生则在此基础上进行调查分析,包含《医务人员手卫生规范》的认知度、七步洗手法的操作、洗手的时机以及影响手卫生的因素等方面。在对共性问题进行分类整理之后,我们将开始实施PBL教学培训。我们将对两组本科实习生在干预前后的手卫生相关知识掌握情况、七步洗手法操作能力、现场暗查手卫生执行率和正确率进行比较。结果 观察组实习生通过情景模拟示教结合PBL教学方法训练,手卫生相关知识方面的掌握情况、七步洗手法的正确率、手卫生执行率和正确率均更高,与对照组实习生相比,组间差别具有统计意义(P<0.05)。结论 通过结合PBL教学法和情景模拟示教,可以全面提升医学本科实习生在手卫生相关知识和七步洗手法正确率方面的表现,从而提高他们的手卫生执行率和正确率,保障患者的安全同时也能增强他们的自我保护意识。展开更多
目的:探讨KAI1对膀胱癌细胞株T-24的增殖和凋亡的影响及相关机制。方法:采用慢病毒感染的方法,使T24细胞株过表达KAI1基因,QPCR及细胞组织化学技术检测T24转染后KAI1的基因及蛋白表达量。MTT技术检测T24细胞的增殖能力;流式细胞技术检...目的:探讨KAI1对膀胱癌细胞株T-24的增殖和凋亡的影响及相关机制。方法:采用慢病毒感染的方法,使T24细胞株过表达KAI1基因,QPCR及细胞组织化学技术检测T24转染后KAI1的基因及蛋白表达量。MTT技术检测T24细胞的增殖能力;流式细胞技术检测细胞周期及T24细胞凋亡情况;Western blotting检测CyclinD1、caspase-3蛋白表达。结果:慢病毒感染后,T24细胞株的KAI1的mRNA转录水平及蛋白的表达水平显著增强(P Objective: To investigate how the KAI1 gene effect the proliferation and apoptosis of bladder cancer cell line T24 and the related mechanism. Methods: T24 cell line were over-expressed KAI1 gene by lentivirus infection. The KAI1 expression in infected T24 cell were detected by QPCR and cell histochemical technique. MTT technique was used to detect the proliferation ability of T24 cells. The cell cycle and apoptosis of T24 cells were detected by flow cytometry. The expression of CyclinD1 and caspase-3 protein was detected by Western blotting. Results: After virus infection, the expression level of mRNA and protein in T24 cell line KAI1 was significantly increased (P < 0.01);cell proliferation was significantly lower than that of no load group and blank control group (P < 0.01);cell apoptosis was significantly higher than those in no load group and blank control group (P < 0.01);cell cycle arrest in G0/G1 phase, S phase cells significantly decreased (P < 0.01);the expression of CyclinD1 protein was down regulated, while caspase-3 protein expression was high regulated. Conclusion: overexpression of KAI1 can significantly reduce the proliferation of bladder cancer cell line T24, enhance cell apoptosis. This effect is achieved by reducing intracellular CyclinD1 protein expression and upregulation of caspase-3 protein.展开更多
目的观察自拟清肝养血汤熏蒸联合揿针治疗肝肾阴虚型干眼症的临床疗效。方法选取肝肾阴虚型睑板腺功能障碍性干眼症患者200例,随机分为治疗组(100例)和对照组(100例)。治疗组采用自拟清肝养血汤熏蒸联合揿针治疗;对照组采用睑板腺按摩...目的观察自拟清肝养血汤熏蒸联合揿针治疗肝肾阴虚型干眼症的临床疗效。方法选取肝肾阴虚型睑板腺功能障碍性干眼症患者200例,随机分为治疗组(100例)和对照组(100例)。治疗组采用自拟清肝养血汤熏蒸联合揿针治疗;对照组采用睑板腺按摩疗法。对比两组患者治疗前、治疗后及治疗后1个月的西医临床积分、中医证候积分、泪液分泌试验(Schirmer I test,SIT)、泪膜破裂时间(Tear film break-up time,BUT)、泪河高度(Tear Meniscus Height,TMH)、睑板腺功能评定等改善情况,并评定两组疗效。结果治疗前两组患者各项评分均相当,差异无统计学意义(P>0.05);治疗后同组对比,各项评分均较治疗前改善,差异具有统计学意义(P<0.05),其中治疗组患者西医临床积分、中医证候积分、睑板腺分泌物性状评分改善程度明显优于对照组(P<0.05),在改善SIT、BUT、TMH方面,两组间对比差异无统计学意义(P>0.05),而对照组改善睑板腺分泌功能评分程度优于治疗组,差异具有统计学意义(P<0.05);治疗后1个月,治疗组各项评分均较治疗后进一步改善(P<0.05),而对照组各项评分均较治疗后改善不明显(P>0.05);除睑板腺分泌功能评分两组间对比无统计学意义(P>0.05),其余各项评分两组间对比治疗组均优于对照组(P<0.05);治疗组患者治疗后总有效率为91.7%,明显高于对照组的81.2%(P<0.05)。结论自拟清肝养血汤熏蒸联合揿针治疗肝肾阴虚型睑板腺功能障碍性干眼症具有显著疗效,可有效改善患者临床症状及体征,且疗效持久。展开更多
文摘目的:探讨KAI1对膀胱癌细胞株T-24的增殖和凋亡的影响及相关机制。方法:采用慢病毒感染的方法,使T24细胞株过表达KAI1基因,QPCR及细胞组织化学技术检测T24转染后KAI1的基因及蛋白表达量。MTT技术检测T24细胞的增殖能力;流式细胞技术检测细胞周期及T24细胞凋亡情况;Western blotting检测CyclinD1、caspase-3蛋白表达。结果:慢病毒感染后,T24细胞株的KAI1的mRNA转录水平及蛋白的表达水平显著增强(P Objective: To investigate how the KAI1 gene effect the proliferation and apoptosis of bladder cancer cell line T24 and the related mechanism. Methods: T24 cell line were over-expressed KAI1 gene by lentivirus infection. The KAI1 expression in infected T24 cell were detected by QPCR and cell histochemical technique. MTT technique was used to detect the proliferation ability of T24 cells. The cell cycle and apoptosis of T24 cells were detected by flow cytometry. The expression of CyclinD1 and caspase-3 protein was detected by Western blotting. Results: After virus infection, the expression level of mRNA and protein in T24 cell line KAI1 was significantly increased (P < 0.01);cell proliferation was significantly lower than that of no load group and blank control group (P < 0.01);cell apoptosis was significantly higher than those in no load group and blank control group (P < 0.01);cell cycle arrest in G0/G1 phase, S phase cells significantly decreased (P < 0.01);the expression of CyclinD1 protein was down regulated, while caspase-3 protein expression was high regulated. Conclusion: overexpression of KAI1 can significantly reduce the proliferation of bladder cancer cell line T24, enhance cell apoptosis. This effect is achieved by reducing intracellular CyclinD1 protein expression and upregulation of caspase-3 protein.
文摘目的观察自拟清肝养血汤熏蒸联合揿针治疗肝肾阴虚型干眼症的临床疗效。方法选取肝肾阴虚型睑板腺功能障碍性干眼症患者200例,随机分为治疗组(100例)和对照组(100例)。治疗组采用自拟清肝养血汤熏蒸联合揿针治疗;对照组采用睑板腺按摩疗法。对比两组患者治疗前、治疗后及治疗后1个月的西医临床积分、中医证候积分、泪液分泌试验(Schirmer I test,SIT)、泪膜破裂时间(Tear film break-up time,BUT)、泪河高度(Tear Meniscus Height,TMH)、睑板腺功能评定等改善情况,并评定两组疗效。结果治疗前两组患者各项评分均相当,差异无统计学意义(P>0.05);治疗后同组对比,各项评分均较治疗前改善,差异具有统计学意义(P<0.05),其中治疗组患者西医临床积分、中医证候积分、睑板腺分泌物性状评分改善程度明显优于对照组(P<0.05),在改善SIT、BUT、TMH方面,两组间对比差异无统计学意义(P>0.05),而对照组改善睑板腺分泌功能评分程度优于治疗组,差异具有统计学意义(P<0.05);治疗后1个月,治疗组各项评分均较治疗后进一步改善(P<0.05),而对照组各项评分均较治疗后改善不明显(P>0.05);除睑板腺分泌功能评分两组间对比无统计学意义(P>0.05),其余各项评分两组间对比治疗组均优于对照组(P<0.05);治疗组患者治疗后总有效率为91.7%,明显高于对照组的81.2%(P<0.05)。结论自拟清肝养血汤熏蒸联合揿针治疗肝肾阴虚型睑板腺功能障碍性干眼症具有显著疗效,可有效改善患者临床症状及体征,且疗效持久。
