乳酸作为肿瘤微环境中的关键代谢产物,在促进肿瘤进展方面发挥重要作用。本研究系统探讨了乳酸调控血小板衍生微粒(platelet-derived microparticles, PMPs)对人肺腺癌生物学行为的影响及其潜在机制。通过CCK-8实验和细胞划痕实验发现,...乳酸作为肿瘤微环境中的关键代谢产物,在促进肿瘤进展方面发挥重要作用。本研究系统探讨了乳酸调控血小板衍生微粒(platelet-derived microparticles, PMPs)对人肺腺癌生物学行为的影响及其潜在机制。通过CCK-8实验和细胞划痕实验发现,不同浓度乳酸显著促进了A549细胞的增殖和迁移能力。此外,在乳酸环境下,PMPs进一步增强了细胞的增殖与迁移,并显著提升了肿瘤相关因子(如VEGF和TGF-β)的分泌水平。本研究揭示了乳酸环境下PMPs对肿瘤的协同作用,为进一步理解乳酸和PMPs在肿瘤微环境中的作用提供了新的视角。Lactate, as a pivotal metabolite in the tumour microenvironment, significantly contributes to tumour growth. This study comprehensively examined the impact of lactate-regulated platelet-derived microparticles (PMPs) on the biological behaviours of human lung adenocarcinoma and their probable causes. The CCK-8 assay and cell scratch assay revealed that varying doses of lactic acid considerably enhanced the proliferation and migratory capacity of A549 cells. Moreover, PMPs augmented cell proliferation and migration while markedly increasing the secretion levels of tumour-associated factors (e.g., VEGF and TGF-β) in a lactic acid environment. This study demonstrated the synergistic effects of PMPs on tumours within a lactic acid milieu, offering a novel perspective for comprehending the roles of lactic acid and PMPs in the tumour microenvironment.展开更多
文摘乳酸作为肿瘤微环境中的关键代谢产物,在促进肿瘤进展方面发挥重要作用。本研究系统探讨了乳酸调控血小板衍生微粒(platelet-derived microparticles, PMPs)对人肺腺癌生物学行为的影响及其潜在机制。通过CCK-8实验和细胞划痕实验发现,不同浓度乳酸显著促进了A549细胞的增殖和迁移能力。此外,在乳酸环境下,PMPs进一步增强了细胞的增殖与迁移,并显著提升了肿瘤相关因子(如VEGF和TGF-β)的分泌水平。本研究揭示了乳酸环境下PMPs对肿瘤的协同作用,为进一步理解乳酸和PMPs在肿瘤微环境中的作用提供了新的视角。Lactate, as a pivotal metabolite in the tumour microenvironment, significantly contributes to tumour growth. This study comprehensively examined the impact of lactate-regulated platelet-derived microparticles (PMPs) on the biological behaviours of human lung adenocarcinoma and their probable causes. The CCK-8 assay and cell scratch assay revealed that varying doses of lactic acid considerably enhanced the proliferation and migratory capacity of A549 cells. Moreover, PMPs augmented cell proliferation and migration while markedly increasing the secretion levels of tumour-associated factors (e.g., VEGF and TGF-β) in a lactic acid environment. This study demonstrated the synergistic effects of PMPs on tumours within a lactic acid milieu, offering a novel perspective for comprehending the roles of lactic acid and PMPs in the tumour microenvironment.
