To investigate the anti-inflammatory mechanism of curcumin in THP-1 cells induced by LPS, the effect of curcumin on inflammatory cytokine mRNA expression was assessed by Real-time PCR. Covalent post-translational modi...To investigate the anti-inflammatory mechanism of curcumin in THP-1 cells induced by LPS, the effect of curcumin on inflammatory cytokine mRNA expression was assessed by Real-time PCR. Covalent post-translational modification profiles were determined by a PathScan? Kit. Western blotting analysis verified the PathScan results and the effects of curcumin on nucleus-related protein changes. Annexin V-FITC and PI staining was used to detect early cellular apoptosis by flow cytometry. Anti-inflammatory effects of curcumin were mediated by reducing the levels of IL-1β, IL-6, TNF-α and ICAM-1 mRNA. However, PathScan and Western blotting results showed that curcumin inhibited inflammatory cytokine mRNA expressions by preventing p65 nuclear translocation without interfering with p38 and JNK/MAPK phosphorylation or IκBα degradation. Moreover, curcumin promoted PARP-1 cleavage and inhibited intranuclear lamina protein Lamin B1 expression. Flow cytometry results showed that curcumin enhanced Annexin V-FITC positive cells when LPS induced inflammation. This study provided evidence that curcumin inhibited p65 nuclear translocation and cytokine transcription by regulating nuclear protein structure and function, suggesting that early apoptosis involved the suppression of p65 nuclear translocation.展开更多
基金National Natural Science Foundation of China(Grant No.81202543)
文摘To investigate the anti-inflammatory mechanism of curcumin in THP-1 cells induced by LPS, the effect of curcumin on inflammatory cytokine mRNA expression was assessed by Real-time PCR. Covalent post-translational modification profiles were determined by a PathScan? Kit. Western blotting analysis verified the PathScan results and the effects of curcumin on nucleus-related protein changes. Annexin V-FITC and PI staining was used to detect early cellular apoptosis by flow cytometry. Anti-inflammatory effects of curcumin were mediated by reducing the levels of IL-1β, IL-6, TNF-α and ICAM-1 mRNA. However, PathScan and Western blotting results showed that curcumin inhibited inflammatory cytokine mRNA expressions by preventing p65 nuclear translocation without interfering with p38 and JNK/MAPK phosphorylation or IκBα degradation. Moreover, curcumin promoted PARP-1 cleavage and inhibited intranuclear lamina protein Lamin B1 expression. Flow cytometry results showed that curcumin enhanced Annexin V-FITC positive cells when LPS induced inflammation. This study provided evidence that curcumin inhibited p65 nuclear translocation and cytokine transcription by regulating nuclear protein structure and function, suggesting that early apoptosis involved the suppression of p65 nuclear translocation.
