AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagen...AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagent were compared, so were DNAs from samples treated with different methods. The effects of DNA quality, annealing temperature and activity of Taq DNA polymerase on PCR products were studied also. RESULTS: The SDS method is simpler and cheaper. The fragment of genomic DNA extracted from fresh sample was longer and purer. The annealing temperature and activity of Taq DNA polymerase showed little effect on PCR amplification, while the quality of DNA affected the PCR amplification directly. CONCLUSION: By extracting genomic DNA from fresh sample and PCR amplification, a specific band was obtained.展开更多
目的:建立测定桔梗样品中桔梗皂苷 E 含量的方法。方法:采用 HPLC-ELSD 法,C_(18)柱(3.9mm×300mm,4μm),以乙腈-水为流动相梯度洗脱,流速1.0mL·min^(-1),ELSD 漂移管温度112℃,载气(N_2)流速3.2L·min^(-1)。结果:不同产...目的:建立测定桔梗样品中桔梗皂苷 E 含量的方法。方法:采用 HPLC-ELSD 法,C_(18)柱(3.9mm×300mm,4μm),以乙腈-水为流动相梯度洗脱,流速1.0mL·min^(-1),ELSD 漂移管温度112℃,载气(N_2)流速3.2L·min^(-1)。结果:不同产地的桔梗中均含有桔梗皂苷 E,但含量差别较大,其中以安徽青阳产含量最高(0.26%)。结论:本方法稳定、可靠,桔梗皂苷 E 可作为评价桔梗质量的指标之一。展开更多
文摘AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagent were compared, so were DNAs from samples treated with different methods. The effects of DNA quality, annealing temperature and activity of Taq DNA polymerase on PCR products were studied also. RESULTS: The SDS method is simpler and cheaper. The fragment of genomic DNA extracted from fresh sample was longer and purer. The annealing temperature and activity of Taq DNA polymerase showed little effect on PCR amplification, while the quality of DNA affected the PCR amplification directly. CONCLUSION: By extracting genomic DNA from fresh sample and PCR amplification, a specific band was obtained.
文摘目的:建立测定桔梗样品中桔梗皂苷 E 含量的方法。方法:采用 HPLC-ELSD 法,C_(18)柱(3.9mm×300mm,4μm),以乙腈-水为流动相梯度洗脱,流速1.0mL·min^(-1),ELSD 漂移管温度112℃,载气(N_2)流速3.2L·min^(-1)。结果:不同产地的桔梗中均含有桔梗皂苷 E,但含量差别较大,其中以安徽青阳产含量最高(0.26%)。结论:本方法稳定、可靠,桔梗皂苷 E 可作为评价桔梗质量的指标之一。