The purpose of this study is to evaluate the interaction effects of In-Chen-How(ArtemisiacapillariesThunb.) on the pharmacokinetics of acetaminophen and on liver microsomal cytochrome P450enzyme activity in rats.The r...The purpose of this study is to evaluate the interaction effects of In-Chen-How(ArtemisiacapillariesThunb.) on the pharmacokinetics of acetaminophen and on liver microsomal cytochrome P450enzyme activity in rats.The rats were divided into control group(n=8) without In-Chen-How and thepretreated group(n=8) administered with In-Chen-How(approximately 1.0 mL.kg-1,according toweight) for5 consecutive days.Rats in the control group received water simultaneously.Each rat was thengiven acetaminophen.The pharmacokinetic parameters of acetaminophen of the two groups weresignificantly different.In the In-Chen-Howpretreated group,the maximumconcentration of acetaminophenand the area under the plasma concentration-time curve were reduced about58.4%,56.7% and 55.4%.To further explain the results,liver microsomal suspensions were obtained from rats that were randomlydivided into control and In-Chen-How pretreated group.The levels of CYP1A2 and CYP2E1 in hepaticmicrosomal protein from pretreated group were increased as compared to that from the control group.Itindicated that In-Chen-How can stimulate the activity of CYP isozymes.The changes in thepharmacokinetics of acetaminophen resulting from the administration of In-Chen-How are related to anincrease in metabolic activity of CYP1A2 and CYP2E1.展开更多
文摘The purpose of this study is to evaluate the interaction effects of In-Chen-How(ArtemisiacapillariesThunb.) on the pharmacokinetics of acetaminophen and on liver microsomal cytochrome P450enzyme activity in rats.The rats were divided into control group(n=8) without In-Chen-How and thepretreated group(n=8) administered with In-Chen-How(approximately 1.0 mL.kg-1,according toweight) for5 consecutive days.Rats in the control group received water simultaneously.Each rat was thengiven acetaminophen.The pharmacokinetic parameters of acetaminophen of the two groups weresignificantly different.In the In-Chen-Howpretreated group,the maximumconcentration of acetaminophenand the area under the plasma concentration-time curve were reduced about58.4%,56.7% and 55.4%.To further explain the results,liver microsomal suspensions were obtained from rats that were randomlydivided into control and In-Chen-How pretreated group.The levels of CYP1A2 and CYP2E1 in hepaticmicrosomal protein from pretreated group were increased as compared to that from the control group.Itindicated that In-Chen-How can stimulate the activity of CYP isozymes.The changes in thepharmacokinetics of acetaminophen resulting from the administration of In-Chen-How are related to anincrease in metabolic activity of CYP1A2 and CYP2E1.
