[Objective] This study aimed to explore the effects of phosphorus on the contents of vitamin E in different cultivars of soybean grains and find the optimum application amount of phosphorus for different genotypes of ...[Objective] This study aimed to explore the effects of phosphorus on the contents of vitamin E in different cultivars of soybean grains and find the optimum application amount of phosphorus for different genotypes of soybean cultivars,in order to increase the contents of vitamin E in soybean grains and improve the qualities.[Method] Three soybean cultivars were selected as experimental materials,including Heinong 48（high-protein cultivar）,Heinong 37（intermediate cultivar） and Heinong 44（high-oil cultivar）.The soybeans were planted in pots,with 0.033 g/kg soil of N and K2O,four phosphorus treatments were set,respectively applied with 0（P1）,0.033（P2）,0.067（P3） and 0.100（P4） g/kg soil of P2O5,and the total contents of vitamin E in different cultivars of soybean grains were determined by high-performance liquid chromatography method.[Result] The total contents of vitamin E in the same cultivar of soybean grains in P3 treatment were significantly higher than that in the other three treatments,the total contents of vitamin E in Heinong 37,Heinong 44 and Heinong 48 in P3 treatment had increased by 11.96%,16.55% and 14.02%,compared with the control;among the three soybean cultivars in P2 treatment,the content of vitamin E in Heinong 37 was the maximum;among the 12 treatment combinations,the total contents of vitamin E in Heinong 44 in P3 treatment was the maximum.The contents of vitamin E in three soybean cultivars significantly varied among the various cultivars and different phosphorus treatments.[Conclusion] Application of phosphorus could affect the contents of vitamin E in three soybean cultivars,appropriate application amount of phosphorus is advantageous to improve the contents of vitamin E in soybean grains.展开更多
甜菜M14品系是在二倍体栽培甜菜染色体组上附加野生白花甜菜第9号染色体的单体附加系,具有耐盐性、无融合生殖等优良性状,是发掘优质基因资源的良好研究材料。实验室前期已利用i TRAQ串联LC-MS/MS技术获得0、200、400 m M Na Cl胁迫下...甜菜M14品系是在二倍体栽培甜菜染色体组上附加野生白花甜菜第9号染色体的单体附加系,具有耐盐性、无融合生殖等优良性状,是发掘优质基因资源的良好研究材料。实验室前期已利用i TRAQ串联LC-MS/MS技术获得0、200、400 m M Na Cl胁迫下叶中差异表达蛋白质76个;与利用Hiseq 2000高通量测序技术构建的Na Cl(0、200、400 m M)胁迫下甜菜M14品系转录组数据库相匹配,得到了3条参与抗氧化酶系统的Bv M14-APX、Bv M14-DHAR3及Bv M14-MDAR的Unigenes序列。对3条Unigenes序列进行生物信息学分析;采用实时荧光定量PCR技术对0、200、400 m M Na Cl胁迫下叶和根中的3条基因进行组织特异性分析,探究3条基因在叶和根中的表达趋势,并比较了叶中3条基因在转录水平和蛋白水平的表达量变化是否一致,借以评估3条基因对Na Cl胁迫的响应。实时荧光定量PCR结果表明,Bv M14-APX基因在200 m M Na Cl胁迫下叶和根中分别上调21.56、3.56倍;400 m M Na Cl胁迫下叶和根中分别上调51.27、11.47倍。Bv M14-DHAR3基因在200 m M Na Cl胁迫下叶和根中分别上调14.52、1.83倍;400m M Na Cl胁迫下叶和根中分别上调22.78、5.1倍。Bv M14-MDAR基因在200 m M Na Cl胁迫下叶和根中分别上调29.04、1.33倍;400 m M Na Cl胁迫下叶和根中分别上调59.3、3.81倍。3条基因在响应Na Cl胁迫过程中,叶中的表达量变化均显著于根,同时叶中转录水平与蛋白表达水平的变化具有一致性,说明这3条基因在抗氧化酶系统中起到了重要的作用,这也为进一步探究Bv M14-APX、Bv M14-DHAR3及Bv M14-MDAR基因间互作关系奠定了基础。展开更多
基金Supported by National Natural Science Foundation of China(31170466)2010 Science and Technology (guidance) Project from Heilongjiang Provincial Department of Education (11553079+3 种基金11553080)Heilongjiang Provincial Natural Science Foundation (C200918)Heilongjiang Provincial Post-doctoral Start-up Research Fund (LBH-Q09022)High-level Personnel Support Project from Heilongjiang University (Ecological restorationteam Hdtd 2010-12)~~
文摘[Objective] This study aimed to explore the effects of phosphorus on the contents of vitamin E in different cultivars of soybean grains and find the optimum application amount of phosphorus for different genotypes of soybean cultivars,in order to increase the contents of vitamin E in soybean grains and improve the qualities.[Method] Three soybean cultivars were selected as experimental materials,including Heinong 48（high-protein cultivar）,Heinong 37（intermediate cultivar） and Heinong 44（high-oil cultivar）.The soybeans were planted in pots,with 0.033 g/kg soil of N and K2O,four phosphorus treatments were set,respectively applied with 0（P1）,0.033（P2）,0.067（P3） and 0.100（P4） g/kg soil of P2O5,and the total contents of vitamin E in different cultivars of soybean grains were determined by high-performance liquid chromatography method.[Result] The total contents of vitamin E in the same cultivar of soybean grains in P3 treatment were significantly higher than that in the other three treatments,the total contents of vitamin E in Heinong 37,Heinong 44 and Heinong 48 in P3 treatment had increased by 11.96%,16.55% and 14.02%,compared with the control;among the three soybean cultivars in P2 treatment,the content of vitamin E in Heinong 37 was the maximum;among the 12 treatment combinations,the total contents of vitamin E in Heinong 44 in P3 treatment was the maximum.The contents of vitamin E in three soybean cultivars significantly varied among the various cultivars and different phosphorus treatments.[Conclusion] Application of phosphorus could affect the contents of vitamin E in three soybean cultivars,appropriate application amount of phosphorus is advantageous to improve the contents of vitamin E in soybean grains.
文摘甜菜M14品系是在二倍体栽培甜菜染色体组上附加野生白花甜菜第9号染色体的单体附加系,具有耐盐性、无融合生殖等优良性状,是发掘优质基因资源的良好研究材料。实验室前期已利用i TRAQ串联LC-MS/MS技术获得0、200、400 m M Na Cl胁迫下叶中差异表达蛋白质76个;与利用Hiseq 2000高通量测序技术构建的Na Cl(0、200、400 m M)胁迫下甜菜M14品系转录组数据库相匹配,得到了3条参与抗氧化酶系统的Bv M14-APX、Bv M14-DHAR3及Bv M14-MDAR的Unigenes序列。对3条Unigenes序列进行生物信息学分析;采用实时荧光定量PCR技术对0、200、400 m M Na Cl胁迫下叶和根中的3条基因进行组织特异性分析,探究3条基因在叶和根中的表达趋势,并比较了叶中3条基因在转录水平和蛋白水平的表达量变化是否一致,借以评估3条基因对Na Cl胁迫的响应。实时荧光定量PCR结果表明,Bv M14-APX基因在200 m M Na Cl胁迫下叶和根中分别上调21.56、3.56倍;400 m M Na Cl胁迫下叶和根中分别上调51.27、11.47倍。Bv M14-DHAR3基因在200 m M Na Cl胁迫下叶和根中分别上调14.52、1.83倍;400m M Na Cl胁迫下叶和根中分别上调22.78、5.1倍。Bv M14-MDAR基因在200 m M Na Cl胁迫下叶和根中分别上调29.04、1.33倍;400 m M Na Cl胁迫下叶和根中分别上调59.3、3.81倍。3条基因在响应Na Cl胁迫过程中,叶中的表达量变化均显著于根,同时叶中转录水平与蛋白表达水平的变化具有一致性,说明这3条基因在抗氧化酶系统中起到了重要的作用,这也为进一步探究Bv M14-APX、Bv M14-DHAR3及Bv M14-MDAR基因间互作关系奠定了基础。
