A new record of Lopadostoma fagi is described in Italy from Fagus sylvatica based on morphological and molecular data.It has effused-pulvinate stromata immersed in the host tissue,with a narrow,black,carbonized encase...A new record of Lopadostoma fagi is described in Italy from Fagus sylvatica based on morphological and molecular data.It has effused-pulvinate stromata immersed in the host tissue,with a narrow,black,carbonized encasement.The ectostromatic disc is visible as a clypeus and surrounded by the reddish brown bark surface.In the combined phylogenetic analysis of ITS,LSU and RPB2 sequence data,the strain derived from the specimen clustered with other L.fagi strains with high bootstrap support.A morphological description with detailed photographs for L.fagi is provided in this study.展开更多
Sexual morph of didymellaceous taxa are characterized by their ascomata with relatively thin peridium,cylindric-clavate to clavate,short-pedicellate or apedicellate asci,hyaline to brown,1-septate to muriform ascospor...Sexual morph of didymellaceous taxa are characterized by their ascomata with relatively thin peridium,cylindric-clavate to clavate,short-pedicellate or apedicellate asci,hyaline to brown,1-septate to muriform ascospores.Its asexual morphs are coelomycetous and comprising pycnidial or acervulus conidiomata,phialidic,hyaline conidiogenous cells and hyaline or pale brown,septate or aseptate conidia.The majority of these cosmopolitan species are plant associated fungi which can be pathogens on a wide range of hosts and some species are of particular relevance for quarantine measures.Recent studies have significantly improved the taxonomy and systematics of didymellaceous taxa based on molecular phylogenetics.In contrast to the accurate and detailed studies on the asexual morphs which are common obligate pathogens,information on their usually saprobic sexual morphs is still limited.Among these phenotypically diverse species,spore characteristics are quite unique as most have hyaline spores with 0-1 septum,while only Neomicrosphaeropsis and Didymellocamarosporium are reported as producing pigmented,muriform spores.These dematiaceous muriform spores are characteristic of a considerable number of species that may be quite divergent in other characters.During taxonomic investigations on the diversity of didymellaceous taxa,we have isolated species from Alhagi pseudalhagi,Coronilla emerus,Cytisus sp.,Elaeagnus angustifolia and Spartium junceum in Italy,Russia and Uzbekistan.A comprehensive phylogeny,based on four loci(ITS,LSU,rpb2 and tub2)is used to infer species relationships.Comprehensive morphological descriptions and in-depth phylogenetic investigations of five new species viz.Ascochyta coronillae-emeri,Microsphaeropsis spartii-juncei,Neomicrosphaeropsis alhagi-pseudalhagi,N.cytisicola and N.elaeagni are presented.展开更多
Two coelomycetous taxa with muriform conidia were collected from Italy,and subjected to morpho-molecular taxonomic analyses.A mega blast search showed that the new taxa had a close relationship with Pseudocamarosporiu...Two coelomycetous taxa with muriform conidia were collected from Italy,and subjected to morpho-molecular taxonomic analyses.A mega blast search showed that the new taxa had a close relationship with Pseudocamarosporium.Maximum-likelihood and Bayesian analyses of combined LSU,SSU and ITS sequence data also showed that these strains reside in Didymosphaeriaceae and cluster with Pseudocamarosporium sensu stricto.Following detailed morphological and molecular analyses,these are introduced as new species in Pseudocamarosporium.The new taxa are illustrated and compared with other known species in the genus.展开更多
During a microfungi survey in the Province of Forlì-Cesena,Italy,a diatrypaceous taxon was collected on a dead branch of Quercus cerris(Fagaceae,Fagales).Phylogenetic analyses of combined ITS andβ-tubulin sequen...During a microfungi survey in the Province of Forlì-Cesena,Italy,a diatrypaceous taxon was collected on a dead branch of Quercus cerris(Fagaceae,Fagales).Phylogenetic analyses of combined ITS andβ-tubulin sequence data identified the taxon as Diatrypella macrospora.This represents a new host and geographical record for D.macrospora.This new collection is similar to the holotype,but differs in having shorter perithecial necks and smaller ascospores with marked curvature.In this account,a detailed description,colour photographs and phylogenetic analyses are provided to represent the new record of D.macrospora.展开更多
This paper provides outlines for Bionectriaceae,Calcarisporiaceae,Hypocreaceae,Nectriaceae,Tilachlidiaceae,Ijuhy-aceae,Stromatonectriaceae and Xanthonectriaceae with taxonomic treatments.We provide up-to-date DNA sequ...This paper provides outlines for Bionectriaceae,Calcarisporiaceae,Hypocreaceae,Nectriaceae,Tilachlidiaceae,Ijuhy-aceae,Stromatonectriaceae and Xanthonectriaceae with taxonomic treatments.We provide up-to-date DNA sequence-based phylogenies including combined gene analysis of ITS,LSU,rpb2,tef1 and tub2 for Hypocreales and accept 17 families.Three new families and 12 new species are introduced with descriptions and illustrations,while 13 new records and one new species combination are provided.Here we mainly detail the taxonomy of Bionectriaceae,Hypocreaceae,Nectriaceae and Tilachlidiaceae,Ijuhyaceae fam.nov.,Stromatonectriaceae fam.nov.and Xanthonectriaceae fam.nov.are introduced in this study based on phenotypic and molecular analyses.For each family we provide a list of accepted genera,the taxo-nomic history,morphological descriptions,taxonomic placement based on DNA sequence data and illustrate the type genus.Representatives of each family are illustrated based on the type herbarium material or fresh specimens where available,or provide relevant references.Notes on ecological and economic importance of the families are also given.展开更多
Demarcation of family,genus and species boundaries in the Diaporthales has been tentative due to uninformative illus-trations and descriptions,overlapping morphological characteristics,misplacement or poor condition o...Demarcation of family,genus and species boundaries in the Diaporthales has been tentative due to uninformative illus-trations and descriptions,overlapping morphological characteristics,misplacement or poor condition of type specimens and shortage of molecular data from ex-type cultures.In this study,we obtained the type specimens or other authentic specimens of diaporthalean taxa from worldwide fungaria.We examined,described and illustrated them.This study is based on morphological characters from type or authentic specimens,details from protologue and original illustrations and molecular data obtained from GenBank.Combined analyses of nrITS,nrLSU,RPB2 and TEF1-a sequence data were used to construct the molecular phylogeny.Additionally,we provided separate phylogenetic trees for families when necessary to show the generic distribution within these families based on suitable gene markers.Based on morphology and phylogeny,we treat 17 genera previously assigned to Diaporthales genera incertae sedis within several families.For some genera we have designated new generic types as they are lacking type species or type species have affiliations with other families.We exclude Anisomycopsis from Diaporthales and place it in Xylariomycetidae genera incertae sedis.Tirisporellaceae,which was previously placed in Tirisporellales is placed in Diaporthales based on phylogeny and morphology.A new combi-nation,Dendrostoma leiphaemia propose for Amphiporthe leiphaemia(Fr.)Butin.Based on the morphological characters and molecular data we accept 27 families and 138 genera within Diaporthales,24 genera in Diaporthales genera incertae sedis and one genus in Xylariomycetidae genera incertae sedis.We provide notes for genera in Diaporthales genera incertae sedis,and excluded and doubtful genera are listed with notes on their taxonomy and phylogeny.展开更多
The ascomycetous families,Didymosphaeriaceae and Montagnulaceae,have been treated in Pleosporales,Dothideomycetes,and both include saprobes,endophytes and pathogens associated with a wide variety of substrates worldwi...The ascomycetous families,Didymosphaeriaceae and Montagnulaceae,have been treated in Pleosporales,Dothideomycetes,and both include saprobes,endophytes and pathogens associated with a wide variety of substrates worldwide.Didymosphaeriaceae was characterized by 1-septate ascospores and trabeculate pseudoparaphyses,mainly anastomosing above the asci,while species in Montagnulaceae had 1 to multi-septate ascospores and generally cellular pseudoparaphyses.In recent treatments,Bimuria,Didymocrea,Kalmusia,Karstenula,Montagnula,Paraphaeosphaeria,Paraconiothyrium and Letendraea were placed in Montagnulaceae,while only Didymosphaeria has been placed in Didymosphaeriaceae.New morphological and molecular data from recent collections have become available and thus the understanding of the families can be improved.Based on analyses of concatenated internal transcribed spacer(ITS)with LSU,SSU andβ-tubulin gene sequences,the taxonomy of the genera classified in these families are reassessed.Our phylogenetic analyses conclude that,the recently introduced Didymosphaeria rubi-ulmifolii with Paraconiothyrium brasi liense species complex,Alloconiothyrium,Bimuria,Deniquelata,Didymocrea,Kalmusia,Karstenula,Letendraea,Montagnula,Neokalmusia,Paraconiothyrium,Paraphaeosphaeria,Phaeodothis and Tremateia,forms a robust clade named here Didymosphaeriaceae.We therefore synonymize Montagnulaceae under Didymosphaeriaceae which is the oldest name and has priority and provide a update account of the family.Didymosphaeria is represented by Didymosphaeria rubi-ulmifolii.A new genus,Neokalmusia,is introduced in the family to accommodate the bambusi colous taxa Kalmusia brevispora and K.scabrispora.This introduction is based on molecular data coupled with morphology.One new species,Paraconiothyrium magnoliae and the sexual morph of Paraconiothyrium fuckelii,is also introduced.Julella and Barria are assigned to the family based on morphological similarity with Didymosphaeriaceae.Wilmia(previously referred to the Phaeosphaeriaceae)is synonymized under Letendraea(Didymosphaeriaceae).Furthermore,a new species,Letendraea cordylinicola,is introduced and placed in Didymosphaeriaceae based on phylogeny and morphology.The paraphyletic nature of Paraconiothyrium is partially resolved,but further sampling with fresh collections and molecular data of species in this genus are needed to obtain a natural classification.展开更多
The genera Lophiostoma,Misturatosphaeria and several other allied taxa in Lophiostomataceae are revisited.Accounts of these taxa,including their history,morphology,and family placement,based on molecular phylogeny,are...The genera Lophiostoma,Misturatosphaeria and several other allied taxa in Lophiostomataceae are revisited.Accounts of these taxa,including their history,morphology,and family placement,based on molecular phylogeny,are provided.Type or representative specimens of Lophiostoma and Misturatosphaeria were examined and fresh specimens were obtained from Germany,Italy,Japan and Thailand.A multi-gene phylogenetic analysis of the lophiostomataceous genera Floricola,Lophiostoma,Misturatosphaeria and related taxa is provided.Sixteen genera including Lophiostoma,Lophiohelichrysum,Dimorphiopsis,Platystomum and Vaginatispora,plus eleven newly introduced genera Biappendiculispora,Alpestrisphaeria,Capulatispora,Coelodictyosporium,Guttulispora,Lophiopoacea,Neotrematosphaeria,Paucispora,Pseudolophiostoma,Pseudoplatystomum and Sigarispora are accepted in Lophiostomataceae based on morphology and phylogeny.Lophiostoma caulium,Lophiostoma arundinis and Lophiostoma caudatum are accommodated in Sigarispora.Lophiostoma winteri and Lophiostoma fuckelii are placed in the genera Lophiopoacea and Vaginatispora respectively.Three Curreya species and Misturatosphaeria claviformis are transferred to a new genus,Neocurreya.All other Misturatosphaeria species except Misturatosphaeria aurantiinotata and M.uniseptata are separated in the new genera Asymmetrispora,Aurantiascoma,Magnibotryascoma,Pseudoaurantiascoma and Pseudomisturatosphaeria based on their morphological and phylogenetic affinities.Another new genus,Ramusculicola is introduced for a new collection from Thailand.These seven new genera are accommodated in a new family Floricolaceae,together with Floricola and Misturatosphaeria.Several massarina-like species clustered as a sister clade to Amorosia littoralis and are accommodated in a new genus Angustimassarina.A new family Amorosiaceae is proposed to accommodate the genera Amorosia and Angustimassarina.The putatively named species Decaisnella formosa and Thyridaria macrostomoides form a separate clade together with a new genus Lignosphaeria which is placed in Dothideomycetes,genera incertae sedis.展开更多
Anthostomella has long been regarded as a large,but polyphyletic genus in the family Xylariaceae,but species in this group generally lack phylogenetic data.In this study,14 anthostomella-like taxa collected from Italy...Anthostomella has long been regarded as a large,but polyphyletic genus in the family Xylariaceae,but species in this group generally lack phylogenetic data.In this study,14 anthostomella-like taxa collected from Italy,were studied using both morphology and molecular data.Single ascospore isolates were obtained and the asexual morphs of five taxa established.The phylogenetic relationships of the xylariaceous taxa were inferred using combined ITS,RPB2,βtubulin and LSU gene regions.We introduce new sequence data for 24 with included the 14 new anthostomella-like taxa.The subfamilies Hypoxyloideae and Xylarioideae within Xylariaceae were recognized as the two major clades with high bootstrap support.Within the two clades 21 subclades were resolved and the anthostomella-like taxa clustered in five of these subclades indicating that the genus is polyphyletic.Anthostomella sensu stricto comprised A.forlicesenica,A.formosa,A.helicofissa,A.rubicola and A.obesa.The A.formosa and A.rubicola collections morphologically closely resemble the type specimens and therefore we designate reference specimens.Three new species Anthostomella helicofissa,A.forlicesenica and A.obesa are also introduced.Four distinct lineages of anthostomella-like taxa correspondent to four new genera,Anthocanalis,Brunneiperidium,Lunatiannulus and Pyriformiascoma,which are also introduced,while one clustered in Astrocystis and is introduced as a new species.Keys to the new anthostomella-like genera and species examined in this study are provided.展开更多
Ophiobolus is a large genus of Phaeosphaeriaceae comprising more than 350 possible species,most of which are saprobes on herbaceous plants in Europe and North America.Ophiobolus species are polyphyletic and the type o...Ophiobolus is a large genus of Phaeosphaeriaceae comprising more than 350 possible species,most of which are saprobes on herbaceous plants in Europe and North America.Ophiobolus species are polyphyletic and the type of Ophiobolus is not represented in GenBank.Therefore,an increased taxon sampling of ophiobolus-like taxa and epitypification of the type species,O.disseminans is reported.Multigene phylogenetic analyses of combined LSU,SSU,TEF1-a and ITS sequence data position O.disseminans in a sister clade with O.ponticus and several Entodesmium species in Phaeosphaeriaceae with high support.Therefore,Entodesmium is synonymized under Ophiobolus.Premilcurensis with it type species,P.senecionis also clusters within the Ophiobolus clade and is synonymized under Ophiobolus.Ophiobolus rossicus sp.nov.is introduced and a reference specimen is designated for O.ponticus.Other ophiobolus-like taxa(Ophiobolus sensu lato)can be distinguished as three main groups,which are introduced as new genera.Ophiobolopsis is introduced to accommodate the new species,Ophiobolopsis italica.The new genus Paraophiobolus is introduced to accommodate P.arundinis sp.nov.and P.plantaginis comb.nov.This genus is characterized by hyaline to pale yellowish ascospores,some green-yellowish at maturity,with a swollen cell,terminal appendages and ascospores not separating into part spores.Pseudoophiobolus gen.nov.is introduced to accommodate six new species and two new combinations,viz.Ps.achilleae,Ps.erythrosporus,Ps.galii,Ps.italicus,Ps.mathieui,Ps.rosae,Ps.subhyalinisporus and Ps.urticicola.Pseudoophiobolus is characterized by subhyaline to pale yellowish or yellowish ascospores,with a swollen cell,lack of terminal appendages and ascospores that do not separate into part spores and is related to Nodulosphaeria.An updated tree for Phaeosphaeriaceae based on multigene analysis is also provided.展开更多
The family Pleosporaceae includes numerous saprobic,opportunistic human,and plant pathogenic taxa.The classification of genera and species Pleosporaceae has been a major challenge due to the lack of a clear understand...The family Pleosporaceae includes numerous saprobic,opportunistic human,and plant pathogenic taxa.The classification of genera and species Pleosporaceae has been a major challenge due to the lack of a clear understanding of the importance of the morphological characters used to distinguish taxa as well as the lack of reference strains.Recent treatments concluded that Pleospora and some other genera in Pleosporaceae are likely polyphyletic.In order to establish the evolutionary relationships and to resolve the polyphyletic nature of Pleospora and allied genera,we sequenced the 18S nrDNA,28S nrDNA,ITS,GAPDH,RPB2 and TEF1-alpha gene regions of Pleosporaceae species and phylogenetically analysed this data.Multigene phylogenies strongly support the monophyletic nature of Pleosporaceae among the other families in Pleosporales,and the acceptance of the genera Alternaria,Bipolaris,Clathrospora,Comoclathris,Curvularia,Dactuliophora,Decorospora,Diademosa,Exserohilum,Extrawettsteinina,Gibbago,Neocamarosporium,Paradendryphiella,Platysporoides,Pleospora,Porocercospora,Pseudoyuconia and Pyrenophora.Austropleospora,Dendryphion,Edenia and Macrospora are excluded from the family based on morphology coupled with molecular data.Two novel species,Alternaria murispora in this paper and Comoclathris sedi are introduced.The sexual morph of Alternaria alternata is re-described and illustrated using modern concepts from fresh collections.The paraphyletic nature of Pleospora is resolved based on the available morpho-molecular data,but further sampling with fresh collections,reference or ex-type strains and molecular data are needed to obtain a natural classification of genera and the family.展开更多
The classification of subclass Xylariomycetidae is revisited with additional collections and phylogeny based on novel rDNA sequence data.Phylogenetic inferences are provided and are based on analysis of 115 sequence d...The classification of subclass Xylariomycetidae is revisited with additional collections and phylogeny based on novel rDNA sequence data.Phylogenetic inferences are provided and are based on analysis of 115 sequence data,including new data for 27 strains.An updated outline to the subclass is presented based on the phylogenies and comprises two orders,18 families and 222 genera.An account of each order,family and genus in the subclass is given.We accept the orders Amphisphaeriales and Xylariales based on morphological and phylogenetic evidence.Amphisphaeriales comprises Amphisphaeriaceae,Bartaliniaceae fam.nov.,Clypeosphaeriaceae,Discosiaceae fam.nov.,Pestalotiopsidaceae fam.nov.and Phlogicylindriaceae fam.nov.Xylariales comprises Apiosporaceae,Cainiaceae,Coniocessiaceae,Diatrypaceae,Graphostromataceae(doubtful),Hyponectriaceae,Iodosphaeriaceae,Lopadostomaceae fam.nov.,Melogrammataceae,Pseudomassariaceae fam.nov.,Vialaeaceae and Xylariaceae.The new genera and species introduced are Arthrinium hyphopodii,A.subglobosa,Cainia anthoxanthis,Ciferriascosea gen.nov.,C.fluctamurum,C.rectamurum,Discosia neofraxinea,D.pseudopleurochaeta,Hyalotiella rubi,Seimatosporium cornii,S.ficeae,S.vitis and Truncatella spartii.展开更多
This paper is a compilation of notes on 110 fungal taxa,including one new family,10 new genera,and 76 new species,representing a wide taxonomic and geographic range.The new family,Paradictyoarthriniaceae is introduced...This paper is a compilation of notes on 110 fungal taxa,including one new family,10 new genera,and 76 new species,representing a wide taxonomic and geographic range.The new family,Paradictyoarthriniaceae is introduced based on its distinct lineage in Dothideomycetes and its unique morphology.The family is sister to Biatriosporaceae and Roussoellaceae.The new genera are Allophaeosphaeria(Phaeosphaeriaceae),Amphibambusa(Amphisphaeriaceae),Brunneomycosphaerella(Capnodiales genera incertae cedis),Chaetocapnodium(Capnodiaceae),Flammeascoma(Anteagloniaceae),Multiseptospora(Pleosporales genera incertae cedis),Neogaeumannomyces(Magnaporthaceae),Palmiascoma(Bambusicolaceae),Paralecia(Squamarinaceae)and Sarimanas(Melanommataceae).The newly described species are the Ascomycota Aliquandostipite manochii,Allophaeosphaeria dactylidis,A.muriformia,Alternaria cesenica,Amphibambusa bambusicola,Amphisphaeria sorbi,Annulohypoxylon thailandicum,Atrotorquata spartii,Brunneomycosphaerella laburni,Byssosphaeria musae,Camarosporium aborescentis,C.aureum,C.frutexensis,Chaetocapnodium siamensis,Chaetothyrium agathis,Colletotrichum sedi,Conicomyces pseudotransvaalensis,Cytospora berberidis,C.sibiraeae,Diaporthe thunbergiicola,Diatrype palmicola,Dictyosporium aquaticum,D.meiosporum,D.thailandicum,Didymella cirsii,Dinemasporium nelloi,Flammeascoma bambusae,Kalmusia italica,K.spartii,Keissleriella sparticola,Lauriomyces synnematicus,Leptosphaeria ebuli,Lophiostoma pseudodictyosporium,L.ravennicum,Lophiotrema eburnoides,Montagnula graminicola,Multiseptospora thailandica,Myrothecium macrosporum,Natantispora unipolaris,Neogaeumannomyces bambusicola,Neosetophoma clematidis,N.italica,Oxydothis atypica,Palmiascoma gregariascomum,Paraconiothyrium nelloi,P.thysanolaenae,Paradictyoarthrinium tectonicola,Paralecia pratorum,Paraphaeosphaeria spartii,Pestalotiopsis digitalis,P.dracontomelon,P.italiana,Phaeoisaria pseudoclematidis,Phragmocapnias philippinensis,Pseudocamarosporium cotinae,Pseudocercospora tamarindi,Pseudotrichia rubriostiolata,P.thailandica,Psiloglonium multiseptatum,Saagaromyces mangrovei,Sarimanas pseudofluviatile,S.shirakamiense,Tothia spartii,Trichomerium siamensis,Wojnowicia dactylidicola,W.dactylidis and W.lonicerae.The Basidiomycota Agaricus flavicentrus,A.hanthanaensis,A.parvibicolor,A.sodalis,Cantharellus luteostipitatus,Lactarius atrobrunneus,L.politus,Phylloporia dependens and Russula cortinarioides are also introduced.Epitypifications or reference specimens are designated for Hapalocystis berkeleyi,Meliola tamarindi,Pallidocercospora acaciigena,Phaeosphaeria musae,Plenodomus agnitus,Psiloglonium colihuae,P.sasicola and Zasmidium musae while notes and/or new sequence data are provided for Annulohypoxylon leptascum,A.nitens,A.stygium,Biscogniauxia marginata,Fasciatispora nypae,Hypoxylon fendleri,H.monticulosum,Leptosphaeria doliolum,Microsphaeropsis olivacea,Neomicrothyrium,Paraleptosphaeria nitschkei,Phoma medicaginis and Saccotheciaceae.A full description of each species is provided with light micrographs(or drawings).Molecular data is provided for 90 taxa and used to generate phylogenetic trees to establish a natural classification for species.展开更多
Leptosphaeriaceae is a family in the order Pleosporales comprising economically important plant pathogens.Species may also be endophytes or saprobes on various host plants.In recent classifications Alternariaster,Lept...Leptosphaeriaceae is a family in the order Pleosporales comprising economically important plant pathogens.Species may also be endophytes or saprobes on various host plants.In recent classifications Alternariaster,Leptosphaeria,Neophaeosphaeria,Paraleptosphaeria,Heterospora,Subplenodomus and Plenodomus were included in the family.The taxonomy of genera and species in Leptosphaeriaceae has been problematic due to the lack of understanding of the importance of morphological characters used to distinguish taxa,as well as the lack of reference strains.In order to establish evolutionary relationships and to provide a backbone tree for Leptosphaeria and allied genera,we sequenced the 18S nrDNA,28S nrDNA,ITS,RPB2,TEF and ACT gene regions of Leptosphaeriaceae species and analysed this data.Multi-locus phylogenies together with morphology robustly support the monophyletic nature of Leptosphaeriaceae among the other families in Pleosporales,and the inclusion of the genera Alternariaster,Heterospora,Leptosphaeria,Paraleptosphaeria,Sphaerellopsis,Subplenodomus,Plenodomus and three novel genera Alloleptosphaeria,Neoleptosphaeria and Pseudoleptosphaeria.Five new species,Alternariaster centaureae-diffusae,Leptosphaeria cichorium,Paraleptosphaeria rubi,Plenodomus guttulatus and P.salviae are introduced.An account of sexual morph of Alternariaster centaureae-diffusae is provided,and the sexual morph of Leptosphaeria doliolum is re-described and illustrated using modern concepts from fresh collections.A novel family Neophaeosphaeriaceae is established to accommodate the genus Neophaeosphaeria and its species.展开更多
Xylariomycetidae(Ascomycota)is a highly diversified group with variable stromatic characters.Our research focused on inconspicuous stromatic xylarialean taxa from China,Italy,Russia,Thailand and the United Kingdom.Det...Xylariomycetidae(Ascomycota)is a highly diversified group with variable stromatic characters.Our research focused on inconspicuous stromatic xylarialean taxa from China,Italy,Russia,Thailand and the United Kingdom.Detailed morpho-logical descriptions,illustrations and combined ITS-LSU-rpb2-tub2-tef1 phylogenies revealed 39 taxa from our collections belonging to Amphisphaeriales and Xylariales.A new family(Appendicosporaceae),five new genera(Magnostiolata,Mela-nostictus,Neoamphisphaeria,Nigropunctata and Paravamsapriya),27 new species(Acrocordiella photiniicola,Allocryp-tovalsa sichuanensis,Amphisphaeria parvispora,Anthostomella lamiacearum,Apiospora guiyangensis,A.sichuanensis,Biscogniauxia magna,Eutypa camelliae,Helicogermslita clypeata,Hypocopra zeae,Magnostiolata mucida,Melanostictus longiostiolatus,M.thailandicus,Nemania longipedicellata,N.delonicis,N.paraphysata,N.thailandensis,Neoamphispha-eria hyalinospora,Neoanthostomella bambusicola,Nigropunctata bambusicola,N.nigrocircularis,N.thailandica,Occul-titheca rosae,Paravamsapriya ostiolata,Peroneutypa leucaenae,Seiridium italicum and Vamsapriya mucosa)and seven new host/geographical records are introduced and reported.Divergence time estimates indicate that Delonicicolales diverged from Amphisphaeriales+Xylariales at 161(123-197)MYA.Amphisphaeriales and Xylariales diverged 154(117-190)MYA with a crown age of 127(92-165)MYA and 147(111-184)MYA,respectively.Appendicosporaceae(Amphisphaeriales)has a stem age of 89(65-117)MYA.Ancestral character state reconstruction indicates that astromatic,clypeate ascomata with aseptate,hyaline ascospores that lack germ slits may probably be ancestral Xylariomycetidae having plant-fungal endo-phytic associations.The Amphisphaeriales remained mostly astromatic with common septate,hyaline ascospores.Stromatic variations may have developed mostly during the Cretaceous period.Brown ascospores are common in Xylariales,but they first appeared in Amphisphaeriaceae,Melogrammataceae and Sporocadaceae during the early Cretaceous.The ascospore germ slits appeared only in Xylariales during the Cretaceous after the divergence of Lopadostomataceae.Hyaline,filiform and apiospores may have appeared as separate lineages,providing the basis for Xylariaceae,which may have diverged inde-pendently.The future classification of polyphyletic xylarialean taxa will not be based on stromatic variations,but the type of ring,the colour of the ascospores,and the presence or absence or the type of germ slit.展开更多
The type specimens or representative specimens of the potentially dothidealean genera Bagnisiella,Botryochora,Coccostromella,Columnosphaeria,Delphinel la,Dictyodothis,Discosphaerina,Dothidea,Dothiora,Endodothiora,Jaff...The type specimens or representative specimens of the potentially dothidealean genera Bagnisiella,Botryochora,Coccostromella,Columnosphaeria,Delphinel la,Dictyodothis,Discosphaerina,Dothidea,Dothiora,Endodothiora,Jaffuela,Mycoporis,Omphalospora,Pachysacca,Plowrightia,Saccothecium,Stylodothis,Sydowia and Yoshinagaia were examined while,fresh specimens of Aureobasidium pullulans,Dothidea insculpta,Plowrightia ribesia and Saccothecium sepincola were made from Italy and Thailand.An introduction and the history of these genera,their family placement,morphology,and molecular phylogeny are provided.Morphology plus GenBank data are used to provide a systematic treatment of Dothideales.Phylogenetic analysis of LSU,SSU and ITS gene regions was carried out and in the resulting phylogenetic tree the taxa cluster in two clades with high bootstrap support.Clade A comprises Dothideaceae,the family type of Dothideales.The family Dothioraceae is not recognized as a distinct family and is synonymized under Dothideaceae.Neocylindroseptoria is introduced to accommodate Cylindroseptoria pistaciae as it forms a well-supported distinct clade in Dothideaceae.Clade B comprises Aureobasidium,Kabatiella,Pseudoseptoria,Saccothecium and Selenophoma species and Columnosphaeria fagi,for which we propose a new family,Aureobasidiaceae.The recently introduced Sydowia eucalypti also clustered within Clade B and therefore based on morphology and molecular phylogeny a new genus Pseudosydowia is introduced for Sydowia eucalypti.Celosporium laricicola is separated in a distinct clade,and therefore it is placed in Dothideales,genera,incertae sedis.The genera Bagnisiella,Botryochora,Coccostromella,Jaffuela,Lucidascocarpa,Mycoporis,Omphalospora,Pachysacca and Yoshinagaia are excluded from Dothideales and their placements are discussed.展开更多
This paper is the seventh in the Fungal Diversity Notes series,where 131 taxa accommodated in 28 families are mainly described from Rosa(Rosaceae)and a few other hosts.Novel fungal taxa are described in the present st...This paper is the seventh in the Fungal Diversity Notes series,where 131 taxa accommodated in 28 families are mainly described from Rosa(Rosaceae)and a few other hosts.Novel fungal taxa are described in the present study,including 17 new genera,93 new species,four combinations,a sexual record for a species and new host records for 16 species.Bhatiellae,Cycasicola,Dactylidina,Embarria,Hawksworthiana,Italica,Melanocucurbitaria,Melanodiplodia,Monoseptella,Uzbekistanica,Neoconiothyrium,Neopaucispora,Pararoussoella,Paraxylaria,Marjia,Sporormurispora and Xenomassariosphaeria are introduced as new ascomycete genera.We also introduce the new species Absidia jindoensis,Alternaria doliconidium,A.hampshirensis,Angustimassarina rosarum,Astragalicola vasilyevae,Backusella locustae,Bartalinia rosicola,Bhatiellae rosae,Broomella rosae,Castanediella camelliae,Coelodictyosporium rosarum,Comoclathris rosae,C.rosarum,Comoclathris rosigena,Coniochaeta baysunika,C.rosae,Cycasicola goaensis,Dactylidina shoemakeri,Dematiopleospora donetzica,D.rosicola,D.salsolae,Diaporthe rosae,D.rosicola,Endoconidioma rosaehissaricae,Epicoccum rosae,Hawksworthiana clematidicola,H.lonicerae,Italica achilleae,Keissleriella phragmiticola,K.rosacearum,K.rosae,K.rosarum,Lophiostoma rosae,Marjia tianschanica,M.uzbekistanica,Melanocucurbitaria uzbekistanica,Melanodiplodia tianschanica,Monoseptella rosae,Mucor fluvius,Muriformistrickeria rosae,Murilentithecium rosae,Neoascochyta rosicola,Neoconiothyrium rosae,Neopaucispora rosaecae,Neosetophoma rosarum,N.rosae,N.rosigena,Neostagonospora artemisiae,Ophiobolus artemisiicola,Paraconiothyrium rosae,Paraphaeosphaeria rosae,P.rosicola,Pararoussoella rosarum,Parathyridaria rosae,Paraxylaria rosacearum,Penicillium acidum,P.aquaticum,Phragmocamarosporium rosae,Pleospora rosae,P.rosae-caninae,Poaceicola agrostina,P.arundinicola,P.rosae,Populocrescentia ammophilae,P.rosae,Pseudocamarosporium pteleae,P.ulmi-minoris,Pseudocercospora rosae,Pseudopithomyces rosae,Pseudostrickeria rosae,Sclerostagonospora lathyri,S.rosae,S.rosicola,Seimatosporium rosigenum,S.rosicola,Seiridium rosarum,Setoseptoria arundelensis,S.englandensis,S.lulworthcovensis,Sigarispora agrostidis,S.caryophyllacearum,S.junci,S.medicaginicola,S.rosicola,S.scrophulariae,S.thymi,Sporormurispora atraphaxidis,S.pruni,Suttonomyces rosae,Umbelopsis sinsidoensis,Uzbekistanica rosaehissaricae,U.yakutkhanika,Wojnowicia rosicola,Xenomassariosphaeria rosae.New host records are provided for Amandinea punctata,Angustimassarina quercicola,Diaporthe rhusicola,D.eres,D.foeniculina,D.rudis,Diplodia seriata,Dothiorella iberica,Lasiodiplodia theobromae,Lecidella elaeochroma,Muriformistrickeria rubi,Neofusicoccum australe,Paraphaeosphaeria michotii,Pleurophoma pleurospora,Sigarispora caulium and Teichospora rubriostiolata.The new combinations are Dactylidina dactylidis(=Allophaeosphaeria dactylidis),Embarria clematidis(=Allophaeosphaeria clematidis),Hawksworthiana alliariae(=Dematiopleospora alliariae)and Italica luzulae(=Dematiopleospora luzulae).This study also provides some insights into the diversity of fungi on Rosa species and especially those on Rosa spines that resulted in the characterisation of eight new genera,45 new species,and nine new host records.We also collected taxa from Rosa stems and there was 31%(20/65)overlap with taxa found on stems with that on spines.Because of the limited and non-targeted sampling for comparison with collections from spines and stems of the same host and location,it is not possible to say that the fungi on spines of Rosa differ from those on stems.The study however,does illustrate how spines are interesting substrates with high fungal biodiversity.This may be because of their hard structure resulting in slow decay and hence are suitable substrates leading to fungal colonisation.All data presented herein are based on morphological examination of specimens,coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.展开更多
Dothideomycetes comprise a highly diverse range of fungi characterized mainly by asci with two wall layers(bitunicate asci)and often with fissitunicate dehiscence.Many species are saprobes,with many asexual states com...Dothideomycetes comprise a highly diverse range of fungi characterized mainly by asci with two wall layers(bitunicate asci)and often with fissitunicate dehiscence.Many species are saprobes,with many asexual states comprising important plant pathogens.They are also endophytes,epiphytes,fungicolous,lichenized,or lichenicolous fungi.They occur in terrestrial,freshwater and marine habitats in almost every part of the world.We accept 105 families in Dothideomycetes with the new families Anteagloniaceae,Bambusicolaceae,Biatriosporaceae,Lichenoconiaceae,Muyocopronaceae,Paranectriellaceae,Roussoellaceae,Salsugineaceae,Seynesiopeltidaceae and Thyridariaceae introduced in this paper.Each family is provided with a description and notes,including asexual and asexual states,and if more than one genus is included,the type genus is also characterized.Each family is provided with at least one figure-plate,usually illustrating the type genus,a list of accepted genera,including asexual genera,and a key to these genera.A phylogenetic tree based on four gene combined analysis add support for 64 of the families and 22 orders,including the novel orders,Dyfrolomycetales,Lichenoconiales,Lichenotheliales,Monoblastiales,Natipusillales,Phaeotrichales and Strigulales.The paper is expected to provide a working document on Dothideomycetes which can be modified as new data comes to light.It is hoped that by illustrating types we provide stimulation and interest so that more work is carried out in this remarkable group of fungi.展开更多
The family Melanommataceae is widespread in temperate and subtropical regions and species invariably occur on twigs or bark of various woody plants in terrestrial,marine or freshwater habitats.In this paper,the type s...The family Melanommataceae is widespread in temperate and subtropical regions and species invariably occur on twigs or bark of various woody plants in terrestrial,marine or freshwater habitats.In this paper,the type species of 26 genera of the family are re-described and illustrated.A multi-gene phylogeny based on maximum likelihood and Bayesian analyses of LSU,SSU,RPB2 and EF-1αsequence data of species of Melanommataceae is provided.The new genera,Muriformistrickeria,Pseudostrickeria and Thysanolaenae are introduced.Anomalemma is synonymized under Exosporiella.Acrocordiopsis,Astrosphaeriella,Beverwykella,Caryosporella,Sporidesmiella and Pseudotrichia are excluded from Melanommataceae based on molecular phylogenetic analyses.Presently,20 genera are accepted in Melanommataceae.Based on the phylogenetic data,five new species,Byssosphaeria siamensis,Herpotrichia vaginatispora,Pseudostrickeria muriformis,Pseudostrickeria ononidis and Muriformistrickeria rubi,are introduced.展开更多
The cosmopolitan plant genus Clematis contains many climbing species that can be found worldwide.The genus occurs in the wild and is grown commercially for horticulture.Microfungi on Clematis were collected from Belgi...The cosmopolitan plant genus Clematis contains many climbing species that can be found worldwide.The genus occurs in the wild and is grown commercially for horticulture.Microfungi on Clematis were collected from Belgium,China,Italy,Thailand and the UK.They are characterized by morphology and analyses of gene sequence data using an integrated species concept to validate identifications.The study revealed two new families,12 new genera,50 new species,26 new host records with one dimorphic character report,and ten species are transferred to other genera.The new families revealed by multigene phylogeny are Longiostiolaceae and Pseudomassarinaceae in Pleosporales(Dothideomycetes).New genera are Anthodidymella(Didymellaceae),Anthosulcatispora and Parasulcatispora(Sulcatisporaceae),Fusiformispora(Amniculicolaceae),Longispora(Phaeosphaeriaceae),Neobyssosphaeria(Melanommataceae),Neoleptosporella(Chaetosphaeriales,genera incertae sedis),Neostictis(Stictidaceae),Pseudohelminthosporium(Neomassarinaceae),Pseudomassarina(Pseudomassarinaceae),Sclerenchymomyces(Leptosphaeriaceae)and Xenoplectosphaerella(Plectosphaerellaceae).The newly described species are Alloleptosphaeria clematidis,Anthodidymella ranunculacearum,Anthosulcatispora subglobosa,Aquadictyospora clematidis,Brunneofusispora clematidis,Chaetosphaeronema clematidicola,C.clematidis,Chromolaenicola clematidis,Diaporthe clematidina,Dictyocheirospora clematidis,Distoseptispora clematidis,Floricola clematidis,Fusiformispora clematidis,Hermatomyces clematidis,Leptospora clematidis,Longispora clematidis,Massariosphaeria clematidis,Melomastia clematidis,M.fulvicomae,Neobyssosphaeria clematidis,Neoleptosporella clematidis,Neoroussoella clematidis,N.fulvicomae,Neostictis nigricans,Neovaginatispora clematidis,Parasulcatispora clematidis,Parathyridaria clematidis,P.serratifoliae,P.virginianae,Periconia verrucose,Phomatospora uniseriata,Pleopunctum clematidis,Pseudocapulatispora clematidis,Pseudocoleophoma clematidis,Pseudohelminthosporium clematidis,Pseudolophiostoma chiangraiense,P.clematidis,Pseudomassarina clematidis,Ramusculicola clematidis,Sarocladium clematidis,Sclerenchymomyces clematidis,Sigarispora clematidicola,S.clematidis,S.montanae,Sordaria clematidis,Stemphylium clematidis,Wojnowiciella clematidis,Xenodidymella clematidis,Xenomassariosphaeria clematidis and Xenoplectosphaerella clematidis.The following fungi are recorded on Clematis species for the first time:Angustimassarina rosarum,Dendryphion europaeum,Dermatiopleospora mariae,Diaporthe ravennica,D.rudis,Dichotomopilus ramosissimum,Dictyocheirospora xishuangbannaensis,Didymosphaeria rubi-ulmifolii,Fitzroyomyces cyperacearum,Fusarium celtidicola,Leptospora thailandica,Memnoniella oblongispora,Neodidymelliopsis longicolla,Neoeutypella baoshanensis,Neoroussoella heveae,Nigrograna chromolaenae,N.obliqua,Pestalotiopsis verruculosa,Pseudoberkleasmium chiangmaiense,Pseudoophiobolus rosae,Pseudoroussoella chromolaenae,P.elaeicola,Ramusculicola thailandica,Stemphylium vesicarium and Torula chromolaenae.The new combinations are Anthodidymella clematidis(≡Didymella clematidis),A.vitalbina(≡Didymella vitalbina),Anthosulcatispora brunnea(≡Neobambusicola brunnea),Fuscohypha kunmingensis(≡Plectosphaerella kunmingensis),Magnibotryascoma rubriostiolata(≡Teichospora rubriostiolata),Pararoussoella mangrovei(≡Roussoella mangrovei),Pseudoneoconiothyrium euonymi(≡Roussoella euonymi),Sclerenchymomyces jonesii(≡Neoleptosphaeria jonesii),Stemphylium rosae(≡Pleospora rosae),and S.rosae-caninae(≡Pleospora rosae-caninae).The microfungi on Clematis is distributed in several classes of Ascomycota.The analyses are based on morphological examination of specimens,coupled with phylogenetic sequence data.To the best of our knowledge,the consolidated species concept approach is recommended in validating species.展开更多
文摘A new record of Lopadostoma fagi is described in Italy from Fagus sylvatica based on morphological and molecular data.It has effused-pulvinate stromata immersed in the host tissue,with a narrow,black,carbonized encasement.The ectostromatic disc is visible as a clypeus and surrounded by the reddish brown bark surface.In the combined phylogenetic analysis of ITS,LSU and RPB2 sequence data,the strain derived from the specimen clustered with other L.fagi strains with high bootstrap support.A morphological description with detailed photographs for L.fagi is provided in this study.
基金the Committee for Coordination Science and Technology Development under the Cabinet of Ministers of Uzbekistan for research support(#P3-2014-0830174425).
文摘Sexual morph of didymellaceous taxa are characterized by their ascomata with relatively thin peridium,cylindric-clavate to clavate,short-pedicellate or apedicellate asci,hyaline to brown,1-septate to muriform ascospores.Its asexual morphs are coelomycetous and comprising pycnidial or acervulus conidiomata,phialidic,hyaline conidiogenous cells and hyaline or pale brown,septate or aseptate conidia.The majority of these cosmopolitan species are plant associated fungi which can be pathogens on a wide range of hosts and some species are of particular relevance for quarantine measures.Recent studies have significantly improved the taxonomy and systematics of didymellaceous taxa based on molecular phylogenetics.In contrast to the accurate and detailed studies on the asexual morphs which are common obligate pathogens,information on their usually saprobic sexual morphs is still limited.Among these phenotypically diverse species,spore characteristics are quite unique as most have hyaline spores with 0-1 septum,while only Neomicrosphaeropsis and Didymellocamarosporium are reported as producing pigmented,muriform spores.These dematiaceous muriform spores are characteristic of a considerable number of species that may be quite divergent in other characters.During taxonomic investigations on the diversity of didymellaceous taxa,we have isolated species from Alhagi pseudalhagi,Coronilla emerus,Cytisus sp.,Elaeagnus angustifolia and Spartium junceum in Italy,Russia and Uzbekistan.A comprehensive phylogeny,based on four loci(ITS,LSU,rpb2 and tub2)is used to infer species relationships.Comprehensive morphological descriptions and in-depth phylogenetic investigations of five new species viz.Ascochyta coronillae-emeri,Microsphaeropsis spartii-juncei,Neomicrosphaeropsis alhagi-pseudalhagi,N.cytisicola and N.elaeagni are presented.
基金the Mushroom Research Foundation(MRF),Chiang Rai Province,Thailand for providing a Postgraduate Scholarship and National Science Funding of China(no.31560489)the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.Erio Camporesi appreciates Giancarlo Lombardi for his invaluable help in the collecting programme and identifying host plants.Yong Wang and Yan-Ling An would like to thank the project of Science and Technology Department of Guizhou Province[2012]4012-06Further,we would like to thank Mae Fah Luang University grant for studying Dothideomycetes(no.56101020032).
文摘Two coelomycetous taxa with muriform conidia were collected from Italy,and subjected to morpho-molecular taxonomic analyses.A mega blast search showed that the new taxa had a close relationship with Pseudocamarosporium.Maximum-likelihood and Bayesian analyses of combined LSU,SSU and ITS sequence data also showed that these strains reside in Didymosphaeriaceae and cluster with Pseudocamarosporium sensu stricto.Following detailed morphological and molecular analyses,these are introduced as new species in Pseudocamarosporium.The new taxa are illustrated and compared with other known species in the genus.
基金We would like to thank Mae Fah Luang University and the Mushroom Research Foundation(MRF),Chiang Rai for financial support.We are grateful to Chunfang Liao for sequencing of sample,Dr.Eleni GentakakiDr.Saranyaphat Boonmee for their great assistance.K.D.Hyde expresses his thanks to the Thailand Research Fund(“Impact of climate change on fungal diversity and biogeography in the Greater Mekong Sub-region RDG6130001”).
文摘During a microfungi survey in the Province of Forlì-Cesena,Italy,a diatrypaceous taxon was collected on a dead branch of Quercus cerris(Fagaceae,Fagales).Phylogenetic analyses of combined ITS andβ-tubulin sequence data identified the taxon as Diatrypella macrospora.This represents a new host and geographical record for D.macrospora.This new collection is similar to the holotype,but differs in having shorter perithecial necks and smaller ascospores with marked curvature.In this account,a detailed description,colour photographs and phylogenetic analyses are provided to represent the new record of D.macrospora.
基金The Research of Featured Microbial Resources and Diversity Investigation in Southwest Karst area(Project No.2014FY120100)is thanked for financial supportKevin D.Hyde acknowledges National Research Council of Thailand(NRCT)grant,Total fungal diversity in a given forest area with implications towards species numbers,chemical diversity and biotechnology(Grant No.N42A650547)+1 种基金This work was also supported by the Princess Srinagarindra’s Centenary Celebrations Foundation(Grant No.64316001)Thailand Science Research and Innovation(TSRI)grant Macrofungi diversity research from the Lancang-Mekong Watershed and Surrounding areas(Grant No.DBG6280009).
文摘This paper provides outlines for Bionectriaceae,Calcarisporiaceae,Hypocreaceae,Nectriaceae,Tilachlidiaceae,Ijuhy-aceae,Stromatonectriaceae and Xanthonectriaceae with taxonomic treatments.We provide up-to-date DNA sequence-based phylogenies including combined gene analysis of ITS,LSU,rpb2,tef1 and tub2 for Hypocreales and accept 17 families.Three new families and 12 new species are introduced with descriptions and illustrations,while 13 new records and one new species combination are provided.Here we mainly detail the taxonomy of Bionectriaceae,Hypocreaceae,Nectriaceae and Tilachlidiaceae,Ijuhyaceae fam.nov.,Stromatonectriaceae fam.nov.and Xanthonectriaceae fam.nov.are introduced in this study based on phenotypic and molecular analyses.For each family we provide a list of accepted genera,the taxo-nomic history,morphological descriptions,taxonomic placement based on DNA sequence data and illustrate the type genus.Representatives of each family are illustrated based on the type herbarium material or fresh specimens where available,or provide relevant references.Notes on ecological and economic importance of the families are also given.
基金National Research Council of Thailand(Mae Fah Luang University)for the grant"Biodiversity,phylogeny and role of fungal endophytes of Pandanaceae"(Grant No.592010200112)Thailand Research Fund(TRF)grant no RSA5980068 entitled"Biodiversity,phylogeny and role of fungal endophytes on above parts of Rhizophora apiculata and Nypa fruticans",the National Research Council of Thailand(Mae Fah Luang University)Grant No.60201000201 entitled"Diseases of mangrove trees and maintenance of good forestry practice"+1 种基金the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.Samantha C.Karunarathna thanks CAS President’s International Fellowship Initiative(PIFI)for funding his postdoctoral research(number 2018PC0006)the National Science Foundation of China(NSFC)for funding this work under the project code 31750110478。
文摘Demarcation of family,genus and species boundaries in the Diaporthales has been tentative due to uninformative illus-trations and descriptions,overlapping morphological characteristics,misplacement or poor condition of type specimens and shortage of molecular data from ex-type cultures.In this study,we obtained the type specimens or other authentic specimens of diaporthalean taxa from worldwide fungaria.We examined,described and illustrated them.This study is based on morphological characters from type or authentic specimens,details from protologue and original illustrations and molecular data obtained from GenBank.Combined analyses of nrITS,nrLSU,RPB2 and TEF1-a sequence data were used to construct the molecular phylogeny.Additionally,we provided separate phylogenetic trees for families when necessary to show the generic distribution within these families based on suitable gene markers.Based on morphology and phylogeny,we treat 17 genera previously assigned to Diaporthales genera incertae sedis within several families.For some genera we have designated new generic types as they are lacking type species or type species have affiliations with other families.We exclude Anisomycopsis from Diaporthales and place it in Xylariomycetidae genera incertae sedis.Tirisporellaceae,which was previously placed in Tirisporellales is placed in Diaporthales based on phylogeny and morphology.A new combi-nation,Dendrostoma leiphaemia propose for Amphiporthe leiphaemia(Fr.)Butin.Based on the morphological characters and molecular data we accept 27 families and 138 genera within Diaporthales,24 genera in Diaporthales genera incertae sedis and one genus in Xylariomycetidae genera incertae sedis.We provide notes for genera in Diaporthales genera incertae sedis,and excluded and doubtful genera are listed with notes on their taxonomy and phylogeny.
基金the Mushroom Research Foundation,Chiang Rai,Thailand for fundingthe Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany+4 种基金the Agricultural Science and Technology Foundation Of Guizhou Province(Nos.NY[2013]3042)the International Collaboration Plan of Guizhou Province(No.G[2012]7006)the innovation team construction For Science and Technology Of Guizhou Province(No.[2012]4007)from the Science and Technology Department of Guizhou Province,Chinathe Japan Society for the Promotion of Science(JSPS,25440199)for financial supportsupported by the Distinguished Scientist Fellowship Program(DSFP),King Saud University,Saudi Arabia.
文摘The ascomycetous families,Didymosphaeriaceae and Montagnulaceae,have been treated in Pleosporales,Dothideomycetes,and both include saprobes,endophytes and pathogens associated with a wide variety of substrates worldwide.Didymosphaeriaceae was characterized by 1-septate ascospores and trabeculate pseudoparaphyses,mainly anastomosing above the asci,while species in Montagnulaceae had 1 to multi-septate ascospores and generally cellular pseudoparaphyses.In recent treatments,Bimuria,Didymocrea,Kalmusia,Karstenula,Montagnula,Paraphaeosphaeria,Paraconiothyrium and Letendraea were placed in Montagnulaceae,while only Didymosphaeria has been placed in Didymosphaeriaceae.New morphological and molecular data from recent collections have become available and thus the understanding of the families can be improved.Based on analyses of concatenated internal transcribed spacer(ITS)with LSU,SSU andβ-tubulin gene sequences,the taxonomy of the genera classified in these families are reassessed.Our phylogenetic analyses conclude that,the recently introduced Didymosphaeria rubi-ulmifolii with Paraconiothyrium brasi liense species complex,Alloconiothyrium,Bimuria,Deniquelata,Didymocrea,Kalmusia,Karstenula,Letendraea,Montagnula,Neokalmusia,Paraconiothyrium,Paraphaeosphaeria,Phaeodothis and Tremateia,forms a robust clade named here Didymosphaeriaceae.We therefore synonymize Montagnulaceae under Didymosphaeriaceae which is the oldest name and has priority and provide a update account of the family.Didymosphaeria is represented by Didymosphaeria rubi-ulmifolii.A new genus,Neokalmusia,is introduced in the family to accommodate the bambusi colous taxa Kalmusia brevispora and K.scabrispora.This introduction is based on molecular data coupled with morphology.One new species,Paraconiothyrium magnoliae and the sexual morph of Paraconiothyrium fuckelii,is also introduced.Julella and Barria are assigned to the family based on morphological similarity with Didymosphaeriaceae.Wilmia(previously referred to the Phaeosphaeriaceae)is synonymized under Letendraea(Didymosphaeriaceae).Furthermore,a new species,Letendraea cordylinicola,is introduced and placed in Didymosphaeriaceae based on phylogeny and morphology.The paraphyletic nature of Paraconiothyrium is partially resolved,but further sampling with fresh collections and molecular data of species in this genus are needed to obtain a natural classification.
基金Kevin D.Hyde thanks the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.
文摘The genera Lophiostoma,Misturatosphaeria and several other allied taxa in Lophiostomataceae are revisited.Accounts of these taxa,including their history,morphology,and family placement,based on molecular phylogeny,are provided.Type or representative specimens of Lophiostoma and Misturatosphaeria were examined and fresh specimens were obtained from Germany,Italy,Japan and Thailand.A multi-gene phylogenetic analysis of the lophiostomataceous genera Floricola,Lophiostoma,Misturatosphaeria and related taxa is provided.Sixteen genera including Lophiostoma,Lophiohelichrysum,Dimorphiopsis,Platystomum and Vaginatispora,plus eleven newly introduced genera Biappendiculispora,Alpestrisphaeria,Capulatispora,Coelodictyosporium,Guttulispora,Lophiopoacea,Neotrematosphaeria,Paucispora,Pseudolophiostoma,Pseudoplatystomum and Sigarispora are accepted in Lophiostomataceae based on morphology and phylogeny.Lophiostoma caulium,Lophiostoma arundinis and Lophiostoma caudatum are accommodated in Sigarispora.Lophiostoma winteri and Lophiostoma fuckelii are placed in the genera Lophiopoacea and Vaginatispora respectively.Three Curreya species and Misturatosphaeria claviformis are transferred to a new genus,Neocurreya.All other Misturatosphaeria species except Misturatosphaeria aurantiinotata and M.uniseptata are separated in the new genera Asymmetrispora,Aurantiascoma,Magnibotryascoma,Pseudoaurantiascoma and Pseudomisturatosphaeria based on their morphological and phylogenetic affinities.Another new genus,Ramusculicola is introduced for a new collection from Thailand.These seven new genera are accommodated in a new family Floricolaceae,together with Floricola and Misturatosphaeria.Several massarina-like species clustered as a sister clade to Amorosia littoralis and are accommodated in a new genus Angustimassarina.A new family Amorosiaceae is proposed to accommodate the genera Amorosia and Angustimassarina.The putatively named species Decaisnella formosa and Thyridaria macrostomoides form a separate clade together with a new genus Lignosphaeria which is placed in Dothideomycetes,genera incertae sedis.
基金The authors appreciate the financial support and postgraduate scholarship provided by State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing and the Mushroom Research Foundation,Chiang Mai,Thailand.The authors gratefully thank Dr Shaun Pennycook from Landcare Research University of Auckland,New Zealand for nomenclature advice on the proposed names.
文摘Anthostomella has long been regarded as a large,but polyphyletic genus in the family Xylariaceae,but species in this group generally lack phylogenetic data.In this study,14 anthostomella-like taxa collected from Italy,were studied using both morphology and molecular data.Single ascospore isolates were obtained and the asexual morphs of five taxa established.The phylogenetic relationships of the xylariaceous taxa were inferred using combined ITS,RPB2,βtubulin and LSU gene regions.We introduce new sequence data for 24 with included the 14 new anthostomella-like taxa.The subfamilies Hypoxyloideae and Xylarioideae within Xylariaceae were recognized as the two major clades with high bootstrap support.Within the two clades 21 subclades were resolved and the anthostomella-like taxa clustered in five of these subclades indicating that the genus is polyphyletic.Anthostomella sensu stricto comprised A.forlicesenica,A.formosa,A.helicofissa,A.rubicola and A.obesa.The A.formosa and A.rubicola collections morphologically closely resemble the type specimens and therefore we designate reference specimens.Three new species Anthostomella helicofissa,A.forlicesenica and A.obesa are also introduced.Four distinct lineages of anthostomella-like taxa correspondent to four new genera,Anthocanalis,Brunneiperidium,Lunatiannulus and Pyriformiascoma,which are also introduced,while one clustered in Astrocystis and is introduced as a new species.Keys to the new anthostomella-like genera and species examined in this study are provided.
基金Rungtiwa Phookamsak thanks to the CAS President’s International Fellowship for Postdoctoral Researchers(Project No.2017PB0072)the Research Fund from China Postdoctoral Science Foundation(grant no.Y71B283261)+2 种基金Chiang Mai University for financial support.Kevin D.Hyde would like to thank the Thailand Research Fund grant no RSA5980068 entitled Biodiversity,phylogeny and role of fungal endophytes on above parts of Rhizophora apiculata and Nypa fruticans and the Chinese Academy of Sciences,Project No.2013T2S0030for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.Financial support by the German Academic Exchange Service(DAAD)and the Thai Royal Golden Ph.D.Jubilee-Industry program(RGJ)for a joint TRF-DAAD PPP(2012-2014)academic exchange grant to K.D.Hyde and M.Stadler.Peter E Mortimer thanks to the National Science Foundation of China(NSFC)for funding this work under the project codes 41761144055 and 41771063Chayanard Phukhamsakda(PHD/0020/2557)acknowledges the Royal Golden Jubilee Ph.D.Program under the Thailand Research Fund.Shi-ke Huang is particularly grateful to Dr.Qi Zhao for his support and great help.Qiu Ju Shang,Jun-Fu Li and Nimali I.de Silva and other colleagues from Centre of Excellence in Fungal Research,Mae Fah Luang University,Chiang Rai,Thailand are thanked for their assistance in phylogenetic analyses.Shaun Pennycook is thanked for suggestion on fungal names.
文摘Ophiobolus is a large genus of Phaeosphaeriaceae comprising more than 350 possible species,most of which are saprobes on herbaceous plants in Europe and North America.Ophiobolus species are polyphyletic and the type of Ophiobolus is not represented in GenBank.Therefore,an increased taxon sampling of ophiobolus-like taxa and epitypification of the type species,O.disseminans is reported.Multigene phylogenetic analyses of combined LSU,SSU,TEF1-a and ITS sequence data position O.disseminans in a sister clade with O.ponticus and several Entodesmium species in Phaeosphaeriaceae with high support.Therefore,Entodesmium is synonymized under Ophiobolus.Premilcurensis with it type species,P.senecionis also clusters within the Ophiobolus clade and is synonymized under Ophiobolus.Ophiobolus rossicus sp.nov.is introduced and a reference specimen is designated for O.ponticus.Other ophiobolus-like taxa(Ophiobolus sensu lato)can be distinguished as three main groups,which are introduced as new genera.Ophiobolopsis is introduced to accommodate the new species,Ophiobolopsis italica.The new genus Paraophiobolus is introduced to accommodate P.arundinis sp.nov.and P.plantaginis comb.nov.This genus is characterized by hyaline to pale yellowish ascospores,some green-yellowish at maturity,with a swollen cell,terminal appendages and ascospores not separating into part spores.Pseudoophiobolus gen.nov.is introduced to accommodate six new species and two new combinations,viz.Ps.achilleae,Ps.erythrosporus,Ps.galii,Ps.italicus,Ps.mathieui,Ps.rosae,Ps.subhyalinisporus and Ps.urticicola.Pseudoophiobolus is characterized by subhyaline to pale yellowish or yellowish ascospores,with a swollen cell,lack of terminal appendages and ascospores that do not separate into part spores and is related to Nodulosphaeria.An updated tree for Phaeosphaeriaceae based on multigene analysis is also provided.
基金MFLU grant number 56101020032 is thanked for supporting studies on Dothideomycetes.We are grateful to the Mushroom Research Foundation,Chiang Rai,Thailand for supporting studies on Dothideomycetes.Kevin D.Hyde thanks the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.Jian-Chu Xu and Peter E Mortimer would like to thank Humidtropics,a CGIAR Research Program that aims to develop new opportunities for improved livelihoods in a sustainable environment,for partially funding this work.H.A Ariyawansa and J.C.Kang are grateful to the agricultural science and technology foundation of Guizhou province(Nos.NY[2013]3042),the international collaboration plan of Guizhou province(No.G[2012]7006)and the innovation team construction for science and technology of Guizhou province(No.[2012]4007)from the Science and Technology Department of Guizhou province,China.Hiran Ariyawansa is grateful to A.D Ariyawansa,D.M.K Ariyawansa and Dhanuska Udayanga for their valuable suggestions.E.B.Gareth Jones is supported by the Distinguished Scientist Fellowship Program(DSFP),King Saud University,Saudi Arabia.
文摘The family Pleosporaceae includes numerous saprobic,opportunistic human,and plant pathogenic taxa.The classification of genera and species Pleosporaceae has been a major challenge due to the lack of a clear understanding of the importance of the morphological characters used to distinguish taxa as well as the lack of reference strains.Recent treatments concluded that Pleospora and some other genera in Pleosporaceae are likely polyphyletic.In order to establish the evolutionary relationships and to resolve the polyphyletic nature of Pleospora and allied genera,we sequenced the 18S nrDNA,28S nrDNA,ITS,GAPDH,RPB2 and TEF1-alpha gene regions of Pleosporaceae species and phylogenetically analysed this data.Multigene phylogenies strongly support the monophyletic nature of Pleosporaceae among the other families in Pleosporales,and the acceptance of the genera Alternaria,Bipolaris,Clathrospora,Comoclathris,Curvularia,Dactuliophora,Decorospora,Diademosa,Exserohilum,Extrawettsteinina,Gibbago,Neocamarosporium,Paradendryphiella,Platysporoides,Pleospora,Porocercospora,Pseudoyuconia and Pyrenophora.Austropleospora,Dendryphion,Edenia and Macrospora are excluded from the family based on morphology coupled with molecular data.Two novel species,Alternaria murispora in this paper and Comoclathris sedi are introduced.The sexual morph of Alternaria alternata is re-described and illustrated using modern concepts from fresh collections.The paraphyletic nature of Pleospora is resolved based on the available morpho-molecular data,but further sampling with fresh collections,reference or ex-type strains and molecular data are needed to obtain a natural classification of genera and the family.
基金S.Maharachchikumbura thanks the Featured microbial resources and diversity investigation in Southwest Karst area(2014FY120100)for funding.The authors extend their sincere appreciations to the Deanship of Scientific Research at King Saud University for its funding this Prolific Research Group(PRG-1436-09).Kevin D.Hyde thanks the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.Y.P.Xiao and T.C.Wen are grateful to The National Natural Science Foundation of China(No.31460012&No.31200016).Shi-Ke Huang and J.C.Kang are grateful to the Agricultural Science and Technology Foundation of Guizhou Province Nos.NY[2013]3042),the International Collaboration Plan of Guizhou Province(No.G[2012]7006)and the Innovation Team Construction for Science And Technology of Guizhou Province(No.[2012]4007)from the Science and Technology Department of Guizhou Province,China.
文摘The classification of subclass Xylariomycetidae is revisited with additional collections and phylogeny based on novel rDNA sequence data.Phylogenetic inferences are provided and are based on analysis of 115 sequence data,including new data for 27 strains.An updated outline to the subclass is presented based on the phylogenies and comprises two orders,18 families and 222 genera.An account of each order,family and genus in the subclass is given.We accept the orders Amphisphaeriales and Xylariales based on morphological and phylogenetic evidence.Amphisphaeriales comprises Amphisphaeriaceae,Bartaliniaceae fam.nov.,Clypeosphaeriaceae,Discosiaceae fam.nov.,Pestalotiopsidaceae fam.nov.and Phlogicylindriaceae fam.nov.Xylariales comprises Apiosporaceae,Cainiaceae,Coniocessiaceae,Diatrypaceae,Graphostromataceae(doubtful),Hyponectriaceae,Iodosphaeriaceae,Lopadostomaceae fam.nov.,Melogrammataceae,Pseudomassariaceae fam.nov.,Vialaeaceae and Xylariaceae.The new genera and species introduced are Arthrinium hyphopodii,A.subglobosa,Cainia anthoxanthis,Ciferriascosea gen.nov.,C.fluctamurum,C.rectamurum,Discosia neofraxinea,D.pseudopleurochaeta,Hyalotiella rubi,Seimatosporium cornii,S.ficeae,S.vitis and Truncatella spartii.
基金The research was supported by 1)Chinese Academy of Sciences(Project No.KSCX2-EW-Z-9).K.Tanaka would like to thank the Japan Society for the Promotion of Science(JSPS,25440199)for financial support.This project was supported by the Distinguished Scientist Fellowship Program(DSFP),King Saud University,Saudi Arabia.This project was also supported by NSTIP strategic technologies program,number(12-BIO2840-02)in the Kingdom of Saudi Arabia.B.Buyck and V.Hofstetter acknowledge the ATM“Emergences”(Dirs.P.Janvier&S.Peigné)from the National museum of natural history,Paris,for financial support related to sequencing as well as travel expenses in Madagascar.Overseas travel of B.Buyck was funded by the ATM research program“Etat et structure phylogénétique de la biodiversitéactuelle et fossile”of the Paris’Museum.(director Ph.Janvier)S.Adamčík acknowledges funding by his national Slovak Project VEGA 02/0075/14.B.D.Shenoy acknowledges funding to visit Mae Fah Luang University,Chiang Rai under Indo-Thailand bilateral programme(THAI-1205).The Lewis family is warmly thanked for their generous hospitality,offering excellent and very agreeable working and field condition.K.Wisitrassameewong and A.Verbeken were financially supported by the joint doctorate program of the“Bijzonder Onderzoeksfonds Gent University”(BOF),Gent University.This research was also financial supported by The Biodiversity and Training Program(BRT R 149009)and Interaction Laboratory(BMIT),National Center for Genetic Engineering and Biotechnology(BIOTEC)Khlong Luang,Pathum Thani 12120,Thailand.Thanks are extended to the Thailand Research Fund grant(BRG 5580009)under the research grant entitled“Taxonomy,Phylogeny and Biochemistry of Thai Basidiomycetes”for financial support.J.Chen and R.L.Zhao would like to thank the National Natural Science Foundation of China(Project ID:31000013,31360014 and 31470152).Uwe Braun,Walter Jaklitsch,Tom May,Marc Stadler and Jo Taylor,are thanks for commenting on some parts of the manuscript.Shaun Pennycook is thanked for checking and suggesting corrections to most of the Latin names.
文摘This paper is a compilation of notes on 110 fungal taxa,including one new family,10 new genera,and 76 new species,representing a wide taxonomic and geographic range.The new family,Paradictyoarthriniaceae is introduced based on its distinct lineage in Dothideomycetes and its unique morphology.The family is sister to Biatriosporaceae and Roussoellaceae.The new genera are Allophaeosphaeria(Phaeosphaeriaceae),Amphibambusa(Amphisphaeriaceae),Brunneomycosphaerella(Capnodiales genera incertae cedis),Chaetocapnodium(Capnodiaceae),Flammeascoma(Anteagloniaceae),Multiseptospora(Pleosporales genera incertae cedis),Neogaeumannomyces(Magnaporthaceae),Palmiascoma(Bambusicolaceae),Paralecia(Squamarinaceae)and Sarimanas(Melanommataceae).The newly described species are the Ascomycota Aliquandostipite manochii,Allophaeosphaeria dactylidis,A.muriformia,Alternaria cesenica,Amphibambusa bambusicola,Amphisphaeria sorbi,Annulohypoxylon thailandicum,Atrotorquata spartii,Brunneomycosphaerella laburni,Byssosphaeria musae,Camarosporium aborescentis,C.aureum,C.frutexensis,Chaetocapnodium siamensis,Chaetothyrium agathis,Colletotrichum sedi,Conicomyces pseudotransvaalensis,Cytospora berberidis,C.sibiraeae,Diaporthe thunbergiicola,Diatrype palmicola,Dictyosporium aquaticum,D.meiosporum,D.thailandicum,Didymella cirsii,Dinemasporium nelloi,Flammeascoma bambusae,Kalmusia italica,K.spartii,Keissleriella sparticola,Lauriomyces synnematicus,Leptosphaeria ebuli,Lophiostoma pseudodictyosporium,L.ravennicum,Lophiotrema eburnoides,Montagnula graminicola,Multiseptospora thailandica,Myrothecium macrosporum,Natantispora unipolaris,Neogaeumannomyces bambusicola,Neosetophoma clematidis,N.italica,Oxydothis atypica,Palmiascoma gregariascomum,Paraconiothyrium nelloi,P.thysanolaenae,Paradictyoarthrinium tectonicola,Paralecia pratorum,Paraphaeosphaeria spartii,Pestalotiopsis digitalis,P.dracontomelon,P.italiana,Phaeoisaria pseudoclematidis,Phragmocapnias philippinensis,Pseudocamarosporium cotinae,Pseudocercospora tamarindi,Pseudotrichia rubriostiolata,P.thailandica,Psiloglonium multiseptatum,Saagaromyces mangrovei,Sarimanas pseudofluviatile,S.shirakamiense,Tothia spartii,Trichomerium siamensis,Wojnowicia dactylidicola,W.dactylidis and W.lonicerae.The Basidiomycota Agaricus flavicentrus,A.hanthanaensis,A.parvibicolor,A.sodalis,Cantharellus luteostipitatus,Lactarius atrobrunneus,L.politus,Phylloporia dependens and Russula cortinarioides are also introduced.Epitypifications or reference specimens are designated for Hapalocystis berkeleyi,Meliola tamarindi,Pallidocercospora acaciigena,Phaeosphaeria musae,Plenodomus agnitus,Psiloglonium colihuae,P.sasicola and Zasmidium musae while notes and/or new sequence data are provided for Annulohypoxylon leptascum,A.nitens,A.stygium,Biscogniauxia marginata,Fasciatispora nypae,Hypoxylon fendleri,H.monticulosum,Leptosphaeria doliolum,Microsphaeropsis olivacea,Neomicrothyrium,Paraleptosphaeria nitschkei,Phoma medicaginis and Saccotheciaceae.A full description of each species is provided with light micrographs(or drawings).Molecular data is provided for 90 taxa and used to generate phylogenetic trees to establish a natural classification for species.
文摘Leptosphaeriaceae is a family in the order Pleosporales comprising economically important plant pathogens.Species may also be endophytes or saprobes on various host plants.In recent classifications Alternariaster,Leptosphaeria,Neophaeosphaeria,Paraleptosphaeria,Heterospora,Subplenodomus and Plenodomus were included in the family.The taxonomy of genera and species in Leptosphaeriaceae has been problematic due to the lack of understanding of the importance of morphological characters used to distinguish taxa,as well as the lack of reference strains.In order to establish evolutionary relationships and to provide a backbone tree for Leptosphaeria and allied genera,we sequenced the 18S nrDNA,28S nrDNA,ITS,RPB2,TEF and ACT gene regions of Leptosphaeriaceae species and analysed this data.Multi-locus phylogenies together with morphology robustly support the monophyletic nature of Leptosphaeriaceae among the other families in Pleosporales,and the inclusion of the genera Alternariaster,Heterospora,Leptosphaeria,Paraleptosphaeria,Sphaerellopsis,Subplenodomus,Plenodomus and three novel genera Alloleptosphaeria,Neoleptosphaeria and Pseudoleptosphaeria.Five new species,Alternariaster centaureae-diffusae,Leptosphaeria cichorium,Paraleptosphaeria rubi,Plenodomus guttulatus and P.salviae are introduced.An account of sexual morph of Alternariaster centaureae-diffusae is provided,and the sexual morph of Leptosphaeria doliolum is re-described and illustrated using modern concepts from fresh collections.A novel family Neophaeosphaeriaceae is established to accommodate the genus Neophaeosphaeria and its species.
基金Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou province,U1812401,Jian-Kui Jack LiuThailand Research,RDG6130001,Kevin D.Hyde+1 种基金Thailand Science Research and Innovation(TSRI)grant,DBG6280009,Kevin D.Hydethe State Research Task of the Subtropical Scientific Centre of the Russian Academy of Sciences(Theme No.0492-2021-0007).
文摘Xylariomycetidae(Ascomycota)is a highly diversified group with variable stromatic characters.Our research focused on inconspicuous stromatic xylarialean taxa from China,Italy,Russia,Thailand and the United Kingdom.Detailed morpho-logical descriptions,illustrations and combined ITS-LSU-rpb2-tub2-tef1 phylogenies revealed 39 taxa from our collections belonging to Amphisphaeriales and Xylariales.A new family(Appendicosporaceae),five new genera(Magnostiolata,Mela-nostictus,Neoamphisphaeria,Nigropunctata and Paravamsapriya),27 new species(Acrocordiella photiniicola,Allocryp-tovalsa sichuanensis,Amphisphaeria parvispora,Anthostomella lamiacearum,Apiospora guiyangensis,A.sichuanensis,Biscogniauxia magna,Eutypa camelliae,Helicogermslita clypeata,Hypocopra zeae,Magnostiolata mucida,Melanostictus longiostiolatus,M.thailandicus,Nemania longipedicellata,N.delonicis,N.paraphysata,N.thailandensis,Neoamphispha-eria hyalinospora,Neoanthostomella bambusicola,Nigropunctata bambusicola,N.nigrocircularis,N.thailandica,Occul-titheca rosae,Paravamsapriya ostiolata,Peroneutypa leucaenae,Seiridium italicum and Vamsapriya mucosa)and seven new host/geographical records are introduced and reported.Divergence time estimates indicate that Delonicicolales diverged from Amphisphaeriales+Xylariales at 161(123-197)MYA.Amphisphaeriales and Xylariales diverged 154(117-190)MYA with a crown age of 127(92-165)MYA and 147(111-184)MYA,respectively.Appendicosporaceae(Amphisphaeriales)has a stem age of 89(65-117)MYA.Ancestral character state reconstruction indicates that astromatic,clypeate ascomata with aseptate,hyaline ascospores that lack germ slits may probably be ancestral Xylariomycetidae having plant-fungal endo-phytic associations.The Amphisphaeriales remained mostly astromatic with common septate,hyaline ascospores.Stromatic variations may have developed mostly during the Cretaceous period.Brown ascospores are common in Xylariales,but they first appeared in Amphisphaeriaceae,Melogrammataceae and Sporocadaceae during the early Cretaceous.The ascospore germ slits appeared only in Xylariales during the Cretaceous after the divergence of Lopadostomataceae.Hyaline,filiform and apiospores may have appeared as separate lineages,providing the basis for Xylariaceae,which may have diverged inde-pendently.The future classification of polyphyletic xylarialean taxa will not be based on stromatic variations,but the type of ring,the colour of the ascospores,and the presence or absence or the type of germ slit.
文摘The type specimens or representative specimens of the potentially dothidealean genera Bagnisiella,Botryochora,Coccostromella,Columnosphaeria,Delphinel la,Dictyodothis,Discosphaerina,Dothidea,Dothiora,Endodothiora,Jaffuela,Mycoporis,Omphalospora,Pachysacca,Plowrightia,Saccothecium,Stylodothis,Sydowia and Yoshinagaia were examined while,fresh specimens of Aureobasidium pullulans,Dothidea insculpta,Plowrightia ribesia and Saccothecium sepincola were made from Italy and Thailand.An introduction and the history of these genera,their family placement,morphology,and molecular phylogeny are provided.Morphology plus GenBank data are used to provide a systematic treatment of Dothideales.Phylogenetic analysis of LSU,SSU and ITS gene regions was carried out and in the resulting phylogenetic tree the taxa cluster in two clades with high bootstrap support.Clade A comprises Dothideaceae,the family type of Dothideales.The family Dothioraceae is not recognized as a distinct family and is synonymized under Dothideaceae.Neocylindroseptoria is introduced to accommodate Cylindroseptoria pistaciae as it forms a well-supported distinct clade in Dothideaceae.Clade B comprises Aureobasidium,Kabatiella,Pseudoseptoria,Saccothecium and Selenophoma species and Columnosphaeria fagi,for which we propose a new family,Aureobasidiaceae.The recently introduced Sydowia eucalypti also clustered within Clade B and therefore based on morphology and molecular phylogeny a new genus Pseudosydowia is introduced for Sydowia eucalypti.Celosporium laricicola is separated in a distinct clade,and therefore it is placed in Dothideales,genera,incertae sedis.The genera Bagnisiella,Botryochora,Coccostromella,Jaffuela,Lucidascocarpa,Mycoporis,Omphalospora,Pachysacca and Yoshinagaia are excluded from Dothideales and their placements are discussed.
基金The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for funding this research work through ISPP#0089.We thank the technical staff of Center of Excellence in Fungal Research,Sornram Sukpisit and Wilawan Punyaboon for their invaluable assistance.Dhanushka Wanasinghe would like to thank the Molecular Biology Experimental Center at Kunming Institute of Botany for facilities for molecular work.We are also grateful to Anuruddha Karunarathna,Binu Samarakoon and Digvijayini Bundhun for their valuable assistance.Dhanushka Wanasinghe is also thankful to Hiran Ariyawansa for his valuable suggestions.Hyang Burm Lee was supported by the Graduate Program for the Undiscovered Taxa of Korea,and the Project on Survey and Discovery of Indigenous Fungal Species of Korea funded by NIBR,and the Project on Discovery of Fungi from Freshwater and Collection of Fungarium funded by NNIBR of the Ministry of Environment(MOE),and the Cooperative Research Program for Agriculture Science and Technology Development(PJ012957)Rural Development Administration,Republic of Korea.Chayanard Phukhamsakda would like to thank Royal Golden Jubilee Ph.D.Program under Thailand Research Fund,for the award of a scholarship no.PHD/0020/2557+6 种基金Ausana Mapook is grateful to Research and Researchers for Industries(RRI)PHD57I0012R.Jeewon is grateful to University of Mauritius&Mae Fah Luang University for enabling research collaboration.K.D.Hyde thanks to National Research Council of Thailand(Mae Fah Luang University)for grants‘‘Biodiversity,phylogeny and role of fungal endophytes of Pandanaceae’’(Grant No:592010200112)and Thailand Research Fund(TRF)Grant No RSA5980068 entitled‘‘Biodiversity,phylogeny and role of fungal endophytes on above parts of Rhizophora apiculata and Nypa fruticans’’.National Research Council of Thailand(Mae Fah Luang University)grant no 60201000201 entitled‘‘Diseases of mangrove trees and maintenance of good forestry practice’’.K.D.Hyde is an Adjunct Professor at Chiang Mai University.Samantha C.Karunarathna thanks Yunnan Provincial Department of Human Resources and Social Security funded postdoctoral project(number 179122)and National Science Foundation of China(NSFC)project code 31750110478Kevin D.Hyde also thanks to the Chinese Academy of Sciences,project number 2013T2S0030for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.Y.S.Gafforov acknowledges the support from Committee for coordination science and technology development under the Cabinet of Ministers of Uzbekistan(Project No.P3-2014-0830174425)Timur Bulgakov appreciates the Alexander Fateryga and T.I.Vyazemsky Karadag Scientific Station(Karadag State Reserve)for the help in expeditions in Crimean Peninsula.Jianchu Xu thanks Key Research Program of Frontier Sciences of the Chinese Academy of Sciences(Grant No.QYZDYSSW-SMC014),Key Research Program of the Ministry of Sciences and Technology(Grant No.2017YFC0505101)CGIAR Research Program 6:Forest,Trees and Agroforestry,the Kunming Institute of Botany,Chinese Academy of Science(CAS)and the Chinese Ministry of Science and Technology,under the 12th 5-year National Key Technology Support Program(NKTSP)2013BAB07B06 integration and comprehensive demonstration of key technologies on Green Phosphate-mountain construction.Peter E.Mortimer thanks the National Science Foundation of China(NSFC)Project Codes 41761144055 and 41771063S.Tibell would like to acknowledge support from the grant‘dha 2016-264.3’from‘The Swedish Taxonomy Initiative’.
文摘This paper is the seventh in the Fungal Diversity Notes series,where 131 taxa accommodated in 28 families are mainly described from Rosa(Rosaceae)and a few other hosts.Novel fungal taxa are described in the present study,including 17 new genera,93 new species,four combinations,a sexual record for a species and new host records for 16 species.Bhatiellae,Cycasicola,Dactylidina,Embarria,Hawksworthiana,Italica,Melanocucurbitaria,Melanodiplodia,Monoseptella,Uzbekistanica,Neoconiothyrium,Neopaucispora,Pararoussoella,Paraxylaria,Marjia,Sporormurispora and Xenomassariosphaeria are introduced as new ascomycete genera.We also introduce the new species Absidia jindoensis,Alternaria doliconidium,A.hampshirensis,Angustimassarina rosarum,Astragalicola vasilyevae,Backusella locustae,Bartalinia rosicola,Bhatiellae rosae,Broomella rosae,Castanediella camelliae,Coelodictyosporium rosarum,Comoclathris rosae,C.rosarum,Comoclathris rosigena,Coniochaeta baysunika,C.rosae,Cycasicola goaensis,Dactylidina shoemakeri,Dematiopleospora donetzica,D.rosicola,D.salsolae,Diaporthe rosae,D.rosicola,Endoconidioma rosaehissaricae,Epicoccum rosae,Hawksworthiana clematidicola,H.lonicerae,Italica achilleae,Keissleriella phragmiticola,K.rosacearum,K.rosae,K.rosarum,Lophiostoma rosae,Marjia tianschanica,M.uzbekistanica,Melanocucurbitaria uzbekistanica,Melanodiplodia tianschanica,Monoseptella rosae,Mucor fluvius,Muriformistrickeria rosae,Murilentithecium rosae,Neoascochyta rosicola,Neoconiothyrium rosae,Neopaucispora rosaecae,Neosetophoma rosarum,N.rosae,N.rosigena,Neostagonospora artemisiae,Ophiobolus artemisiicola,Paraconiothyrium rosae,Paraphaeosphaeria rosae,P.rosicola,Pararoussoella rosarum,Parathyridaria rosae,Paraxylaria rosacearum,Penicillium acidum,P.aquaticum,Phragmocamarosporium rosae,Pleospora rosae,P.rosae-caninae,Poaceicola agrostina,P.arundinicola,P.rosae,Populocrescentia ammophilae,P.rosae,Pseudocamarosporium pteleae,P.ulmi-minoris,Pseudocercospora rosae,Pseudopithomyces rosae,Pseudostrickeria rosae,Sclerostagonospora lathyri,S.rosae,S.rosicola,Seimatosporium rosigenum,S.rosicola,Seiridium rosarum,Setoseptoria arundelensis,S.englandensis,S.lulworthcovensis,Sigarispora agrostidis,S.caryophyllacearum,S.junci,S.medicaginicola,S.rosicola,S.scrophulariae,S.thymi,Sporormurispora atraphaxidis,S.pruni,Suttonomyces rosae,Umbelopsis sinsidoensis,Uzbekistanica rosaehissaricae,U.yakutkhanika,Wojnowicia rosicola,Xenomassariosphaeria rosae.New host records are provided for Amandinea punctata,Angustimassarina quercicola,Diaporthe rhusicola,D.eres,D.foeniculina,D.rudis,Diplodia seriata,Dothiorella iberica,Lasiodiplodia theobromae,Lecidella elaeochroma,Muriformistrickeria rubi,Neofusicoccum australe,Paraphaeosphaeria michotii,Pleurophoma pleurospora,Sigarispora caulium and Teichospora rubriostiolata.The new combinations are Dactylidina dactylidis(=Allophaeosphaeria dactylidis),Embarria clematidis(=Allophaeosphaeria clematidis),Hawksworthiana alliariae(=Dematiopleospora alliariae)and Italica luzulae(=Dematiopleospora luzulae).This study also provides some insights into the diversity of fungi on Rosa species and especially those on Rosa spines that resulted in the characterisation of eight new genera,45 new species,and nine new host records.We also collected taxa from Rosa stems and there was 31%(20/65)overlap with taxa found on stems with that on spines.Because of the limited and non-targeted sampling for comparison with collections from spines and stems of the same host and location,it is not possible to say that the fungi on spines of Rosa differ from those on stems.The study however,does illustrate how spines are interesting substrates with high fungal biodiversity.This may be because of their hard structure resulting in slow decay and hence are suitable substrates leading to fungal colonisation.All data presented herein are based on morphological examination of specimens,coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.
基金We also thank Siriporn Luesuwan for arranging the loan of specimens from various herbaria.A.Ariyawansa and J.C Kang are grateful to the International collaboration plan of Science and Technology at Guizhou Province(contract No.[2012]7006)the construction of innovation talent team of Science and Technology at Guizhou Province(contract No.[2012]4007)+19 种基金China.D.J.Bhat is thankful to MFU for a Visiting Professorship during the tenure of which this paper was finalized.D.L.Hawksworth contributed to this work while in receipt of support from the Spanish Ministerio de Ciencia e Innovación(CGL2011-25003)Haixia Wu would like to thank the Grant for Essential Scientific Research of National Non-profit Institute to funds for research(No.CAFYBB2007002)thanks Xiaoming Chen,Ying Feng and Chen Hang(The Research Institute of Resource Insects,Chinese Academy of Forestry,China)for their valuable help.Jian-Kui Liu would like to thank Manfred Binder for providing valuable suggestions and kind assistance on phylogenetic analysisWe would like to thank MFU grant No.56101020032 for funding to study taxonomy and phylogeny of selected families of DothideomycetesJiye Yan and Xinghong Li would like to thank CARS-30 for funds.K.Tanaka would like to thank the Japan Society for the Promotion of Science(JSPS,25440199)for financial supportK.L.Pang would like to thank National Science Council of Taiwan for financial support(NSC101-2621-B-019-001-MY3).L.Muggia is grateful to the Austrian Science Foundation for financial support(FWF,P24114-B16 and Herta-Firnberg Project T481-B20)M.Doilom would like to thank the Thailand Research Fund through the Royal Golden Jubilee(RGJ)Ph.D.Program grant No.Ph.D./0072/2553 in 4.S.M.F./53/A.2MP Nelsen and R Lücking are grateful to the NSF(DEB 0715660“Neotropical Epiphytic Microlichens-An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms”DEB 0717476“Systematics of Dothideomycetes”)MP Nelsen also acknowledges a Brown Family Fellowship through the Field Museum,a William Harper Rainey Fellowship through the University of Chicago,and support through the Committee on Evolutionary Biology at the University of Chicago.R.Phookamsak would like to thank the Royal Golden Jubilee Ph.D.Program(PHD/0090/2551)under the Thailand Research Fund for scholarship supportS.A.Alias would like to thank Program Rakan University Malaya(PRPUM)-Phylogeny,Taxonomy,Relationships and Biotechnological Potential of Sooty Moulds.S.Boonmee also thanks Amy Y.Rossman and the U.S.Department of Agriculture Agricultural Research Service,Systematic Mycology and Microbiology Lab(SMML)USA for laboratory,funding support and advice on her work.S.Boonmee and P.Chomnunti would like to thank TRF/BIOTEC program Biodiversity Research and Training Grant BRT R_251181,BRT R_253012the Mushroom Research Foundation,Chiang Rai Province for funding support.S.Wikee would like to thank the Thailand Research Fund through the Royal Golden Jubilee Ph.D.Program agreement No PhD/0198/2552S.Wikee and JK Liu would like to thank The National Research Council of Thailand(NRCT)for the award of grant No 55201020002 to study the genus Phyllosticta in ThailandS.Suetrong acknowledges the financial support by TRF/BIOTEC program Biodiversity Research and Training Grant BRT R_351004 and BRT R_325015 to study marine fungi of ThailandSuetrong also thanks Morakot Tanticharoen,Kanyawim Kirtikara and Lily Eurwilaichitr,BIOTEC,Bangkok for their continued interest and support.Supalak Yacharoen,J.Monkai and K.D.Hyde would like to thank the Thailand Research Fund(BRG5280002)for financial supportGareth Jones is supported by the Distinguished Scientist Fellowship Program(DSFP),King Saud University,Saudi Arabia.Y.Wang would like to thank The International Scientific Cooperated Project of Guizhou Province(No[2013]7004)Yongxiang Liu would like to thank the Guizhou Research Fund(QKHZYZ[2010]5031 and QNKYYZX[2012]010)for financial supportHarrie Sipman is thanked for comments on part of the manuscript.
文摘Dothideomycetes comprise a highly diverse range of fungi characterized mainly by asci with two wall layers(bitunicate asci)and often with fissitunicate dehiscence.Many species are saprobes,with many asexual states comprising important plant pathogens.They are also endophytes,epiphytes,fungicolous,lichenized,or lichenicolous fungi.They occur in terrestrial,freshwater and marine habitats in almost every part of the world.We accept 105 families in Dothideomycetes with the new families Anteagloniaceae,Bambusicolaceae,Biatriosporaceae,Lichenoconiaceae,Muyocopronaceae,Paranectriellaceae,Roussoellaceae,Salsugineaceae,Seynesiopeltidaceae and Thyridariaceae introduced in this paper.Each family is provided with a description and notes,including asexual and asexual states,and if more than one genus is included,the type genus is also characterized.Each family is provided with at least one figure-plate,usually illustrating the type genus,a list of accepted genera,including asexual genera,and a key to these genera.A phylogenetic tree based on four gene combined analysis add support for 64 of the families and 22 orders,including the novel orders,Dyfrolomycetales,Lichenoconiales,Lichenotheliales,Monoblastiales,Natipusillales,Phaeotrichales and Strigulales.The paper is expected to provide a working document on Dothideomycetes which can be modified as new data comes to light.It is hoped that by illustrating types we provide stimulation and interest so that more work is carried out in this remarkable group of fungi.
文摘The family Melanommataceae is widespread in temperate and subtropical regions and species invariably occur on twigs or bark of various woody plants in terrestrial,marine or freshwater habitats.In this paper,the type species of 26 genera of the family are re-described and illustrated.A multi-gene phylogeny based on maximum likelihood and Bayesian analyses of LSU,SSU,RPB2 and EF-1αsequence data of species of Melanommataceae is provided.The new genera,Muriformistrickeria,Pseudostrickeria and Thysanolaenae are introduced.Anomalemma is synonymized under Exosporiella.Acrocordiopsis,Astrosphaeriella,Beverwykella,Caryosporella,Sporidesmiella and Pseudotrichia are excluded from Melanommataceae based on molecular phylogenetic analyses.Presently,20 genera are accepted in Melanommataceae.Based on the phylogenetic data,five new species,Byssosphaeria siamensis,Herpotrichia vaginatispora,Pseudostrickeria muriformis,Pseudostrickeria ononidis and Muriformistrickeria rubi,are introduced.
基金Chayanard Phukhamsakda would like to thank the Royal Golden Jubilee PhD Program under Thailand Research Fund(RGJ)and the German Academic Exchange Service(DAAD)for a joint TRF-DAAD(PPP 2017-2018)academic exchange Grant to K.D.Hyde and M.Stadler and the RGJ for a personal grant to C.Phukhamsakda(The scholarship no.PHD/0020/2557 to study towards a PhD).Dr.Shaun Pennycook is thanked for checking and suggesting Latin names of the new taxa.Dr.Olivier Raspé,Dr.Rajesh Jeewon,Sirinapa Konta,Milan Samarakoon,Indunil C.Senanayake,Dr.Sinang Hongsanan,Chuan-Gen Lin,Qiu-Ju Shang and Pranami D.Abeywickrama are thanked for their valuable suggestions on the phylogenetic analysis and for sequencing.Mr.Martin van de Bult,Cyrille Gerstmans,Prof.Hong-Yan Su,Tian Qing,Dr.Zong-Long Luo are gratefully acknowledged for sample collection guidance in China and Thailand.Kevin D.Hyde thanks the future of specialist fungi in a changing climate:baseline data for generalist and specialist fungi associated with ants,Rhododendron species and Dracaena species(Grant No:DBG6080013)Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion(Grant No:RDG6130001)+6 种基金Alan J.L.Phillips acknowledges the support from UID/MULTI/04046/2019 Research Unit grant from FCT,Portugal to BioISI.E.B.Gareth Jones is supported under the Distinguished Scientist Fellowship Program(DSFP),King Saud University,Kingdom of Saudi Arabia.D.N.Wanasinghe would like to thank the CAS President’s International Fellowship Initiative(PIFI)for funding his postdoctoral research(Number 2019PC0008)the National Science Foundation of China and the Chinese Academy of Sciences for financial support under the following Grants:41761144055,41771063 and Y4ZK111B01Wanasinghe also thanks the 64th batch of China Postdoctoral Science Foundation(Grant No.:Y913083271)Saowaluck Tibpromma would like to thanks the International Postdoctoral Exchange Fellowship Program(Number Y9180822S1)CAS President’s International Fellowship Initiative(PIFI)(Number 2020PC0009)China Postdoctoral Science Foundation and the Yunnan Human Resources,and Social Security Department Foundation for funding her postdoctoral research.Mingkwan Doilom would like to thank the 5th batch of Postdoctoral Orientation Training Personnel in Yunnan Province(Grant No.:Y934283261)the 64th batch of China Postdoctoral Science Foundation(Grant No.:Y913082271).
文摘The cosmopolitan plant genus Clematis contains many climbing species that can be found worldwide.The genus occurs in the wild and is grown commercially for horticulture.Microfungi on Clematis were collected from Belgium,China,Italy,Thailand and the UK.They are characterized by morphology and analyses of gene sequence data using an integrated species concept to validate identifications.The study revealed two new families,12 new genera,50 new species,26 new host records with one dimorphic character report,and ten species are transferred to other genera.The new families revealed by multigene phylogeny are Longiostiolaceae and Pseudomassarinaceae in Pleosporales(Dothideomycetes).New genera are Anthodidymella(Didymellaceae),Anthosulcatispora and Parasulcatispora(Sulcatisporaceae),Fusiformispora(Amniculicolaceae),Longispora(Phaeosphaeriaceae),Neobyssosphaeria(Melanommataceae),Neoleptosporella(Chaetosphaeriales,genera incertae sedis),Neostictis(Stictidaceae),Pseudohelminthosporium(Neomassarinaceae),Pseudomassarina(Pseudomassarinaceae),Sclerenchymomyces(Leptosphaeriaceae)and Xenoplectosphaerella(Plectosphaerellaceae).The newly described species are Alloleptosphaeria clematidis,Anthodidymella ranunculacearum,Anthosulcatispora subglobosa,Aquadictyospora clematidis,Brunneofusispora clematidis,Chaetosphaeronema clematidicola,C.clematidis,Chromolaenicola clematidis,Diaporthe clematidina,Dictyocheirospora clematidis,Distoseptispora clematidis,Floricola clematidis,Fusiformispora clematidis,Hermatomyces clematidis,Leptospora clematidis,Longispora clematidis,Massariosphaeria clematidis,Melomastia clematidis,M.fulvicomae,Neobyssosphaeria clematidis,Neoleptosporella clematidis,Neoroussoella clematidis,N.fulvicomae,Neostictis nigricans,Neovaginatispora clematidis,Parasulcatispora clematidis,Parathyridaria clematidis,P.serratifoliae,P.virginianae,Periconia verrucose,Phomatospora uniseriata,Pleopunctum clematidis,Pseudocapulatispora clematidis,Pseudocoleophoma clematidis,Pseudohelminthosporium clematidis,Pseudolophiostoma chiangraiense,P.clematidis,Pseudomassarina clematidis,Ramusculicola clematidis,Sarocladium clematidis,Sclerenchymomyces clematidis,Sigarispora clematidicola,S.clematidis,S.montanae,Sordaria clematidis,Stemphylium clematidis,Wojnowiciella clematidis,Xenodidymella clematidis,Xenomassariosphaeria clematidis and Xenoplectosphaerella clematidis.The following fungi are recorded on Clematis species for the first time:Angustimassarina rosarum,Dendryphion europaeum,Dermatiopleospora mariae,Diaporthe ravennica,D.rudis,Dichotomopilus ramosissimum,Dictyocheirospora xishuangbannaensis,Didymosphaeria rubi-ulmifolii,Fitzroyomyces cyperacearum,Fusarium celtidicola,Leptospora thailandica,Memnoniella oblongispora,Neodidymelliopsis longicolla,Neoeutypella baoshanensis,Neoroussoella heveae,Nigrograna chromolaenae,N.obliqua,Pestalotiopsis verruculosa,Pseudoberkleasmium chiangmaiense,Pseudoophiobolus rosae,Pseudoroussoella chromolaenae,P.elaeicola,Ramusculicola thailandica,Stemphylium vesicarium and Torula chromolaenae.The new combinations are Anthodidymella clematidis(≡Didymella clematidis),A.vitalbina(≡Didymella vitalbina),Anthosulcatispora brunnea(≡Neobambusicola brunnea),Fuscohypha kunmingensis(≡Plectosphaerella kunmingensis),Magnibotryascoma rubriostiolata(≡Teichospora rubriostiolata),Pararoussoella mangrovei(≡Roussoella mangrovei),Pseudoneoconiothyrium euonymi(≡Roussoella euonymi),Sclerenchymomyces jonesii(≡Neoleptosphaeria jonesii),Stemphylium rosae(≡Pleospora rosae),and S.rosae-caninae(≡Pleospora rosae-caninae).The microfungi on Clematis is distributed in several classes of Ascomycota.The analyses are based on morphological examination of specimens,coupled with phylogenetic sequence data.To the best of our knowledge,the consolidated species concept approach is recommended in validating species.