AIM: To determine the role of inflammatory cytokines and reactive oxygen species (ROS) in childhood reflux esophagitis. METHODS: A total of 59 subjects who had complaints suggesting GERD underwent esophagogastroduoden...AIM: To determine the role of inflammatory cytokines and reactive oxygen species (ROS) in childhood reflux esophagitis. METHODS: A total of 59 subjects who had complaints suggesting GERD underwent esophagogastroduoden oscopy. Endoscopic and histopathologic diagnosis of reflux esophagitis was established by Savary-Miller and Vandenplas grading systems, respectively. Esophageal biopsy specimens were taken from the esophagus 20% proximal above the esophagogastric junction for conventional histopathological examination and the measurements of ROS and cytokine levels. ROS were measured by chemiluminescence, whereas IL-8 and MCP-1 levels were determined with quantitative immunometric ELISA on esophageal tissue. Esophagealtissue ROS, IL-8 and MCP-1 levels were compared among groups with and without endoscopic/histo- pathologic esophagitis. RESULTS: Of 59 patients 28 (47.5%) had normal esophagus whereas 31 (52.5%) had endoscopic esophagitis. In histopathological evaluation, almost 73% of the cases had mild and 6.8% had moderate degree of esophagitis. When ROS and chemokine levels were compared among groups with and without endoscopic esophagitis, statistical difference could not be found between patients with and without esophagitis. Although the levels of ROS, IL-8 and MCP-1 were found to be higher in the group with histopathological reflux esophagitis, this difference was not statistically significant. CONCLUSION: These results suggest that the grade of esophagitis is usually mild or moderate during childhood and factors apart from ROS, IL-8 and MCP-1 may be involved in the pathogenesis of reflux esophagitis in children.展开更多
Plant-derived compounds have been recognized by the feed industry as important supplements for livestock welfare and health. In this context, <i>Citrus aurantium</i> L. extract and <i>Origanum vulgar...Plant-derived compounds have been recognized by the feed industry as important supplements for livestock welfare and health. In this context, <i>Citrus aurantium</i> L. extract and <i>Origanum vulgare</i> L. essential oil have been demonstrated to have strong anti-inflammatory and antioxidant effects on animals. Being the composition of plant-derived extracts extremely influenced by the environmental and growing conditions of the plants, quality control is necessary in terms of the concentration of the active compounds to assure the reproducibility of natural feed additives. The present work aimed at the validation of the extraction procedure from feed additives of Hesperidin (HES) and Carvacrol (CAR), the main active compounds of <i>Citrus aurantium</i> and <i>Origanum vulgare</i> extracts. Then, the quantification method of both the analytes was developed and validated by reversed high-performance liquid chromatography coupled with a UV detector. The validated method was tested on premixtures and final feed additives supplied by a local feed factory to supervise the production chain. The extraction method with methanol resulted to be efficient and highly reproducible, with recovery higher than 90% for both the analytes. The chromatographic method has been demonstrated to be accurate, precise (relative standard deviation percent lower than 2.06%), and linear in the tested range concentrations, with regression coefficients equal to 0.995 and 0.999 for HES and CAR respectively. The method demonstrated that the feed additives prepared by the factory by diluting the premixtures were less concentrated than what was declared on the label.展开更多
Somalia Mogadishu-Turkey Training and Research Hospital is only powered by diesel generator currently.In this paper,the energy demand of this hospital is supplied by determining the optimum hybrid power renewable gene...Somalia Mogadishu-Turkey Training and Research Hospital is only powered by diesel generator currently.In this paper,the energy demand of this hospital is supplied by determining the optimum hybrid power renewable generating system.Therefore,numerous hybrid renewable power generating systems including the components like diesel generator,wind turbine,photovoltaic(PV)and battery are considered in different configurations.Eventually,they are technically,environmentally and economically analyzed by using the well-known HOMER software.Furthermore,a sensitivity analysis is also performed considering variations in three important parameters,namely average wind speed,current diesel price and also solar radiation.According to the results,the optimal system is the standalone Wind/Diesel/Battery hybrid renewable energy system(HRES)with the configuration of 1,000 kW wind turbine,350 kW diesel generator,250 kW power converters and 750 batteries.Additionally,this system has the net present cost of$5,056,700 as well as the cost of energy as$0.191/kWh.Lastly,it is clearly occurred that the Wind/Diesel/Battery HRES is eco-friendlier than other HRESs.展开更多
We report a case of a 7- year- old girl with hyperimmunoglobulin- E syndrome presenting with widespread oral papillomas which were tested for human papilloma virus DNA and had shown to be at high/intermediate risk gro...We report a case of a 7- year- old girl with hyperimmunoglobulin- E syndrome presenting with widespread oral papillomas which were tested for human papilloma virus DNA and had shown to be at high/intermediate risk group formalignancy. She had elevated levels of IgE, recurrent sinopulmonary infections, atopic- like dermatitis, peripheral eosinophilia and defective neutrophil chemotaxis. Interferon alfa 2b therapy and chemoprophylaxis with sulfamethoxazole- trimethoprim was given. Although the papillomas partially improved with the treatment, sinopulmonary infections continue to occur.展开更多
Plasma nitrotyrosine and nitrite/nitrate levels as markers of nitrosative stress and plasma malondialdehyde (MDA) and protein carbonyl as markers of oxidative stress were determined in patients with Behc.et disease (B...Plasma nitrotyrosine and nitrite/nitrate levels as markers of nitrosative stress and plasma malondialdehyde (MDA) and protein carbonyl as markers of oxidative stress were determined in patients with Behc.et disease (BD). To evaluate the balance between oxidant and antioxidant systems in these patients, we measurederythrocytelysateCuZnsuperoxidedismutase(CuZn SOD) activity, plasma sulfhydryl (SH) values and total antioxidant activity. We also determined levels of plasma C- reactive protein (CRP), a key marker of inflammation, and compared them with those of healthy subjects. We found plasma nitro- tyrosine levels of BD patients to be increased, indicating that nitrosative stress may occur in these patients. Plasma MDA and CRP levels in BD patients were found to be significantly higher than those in control group. However, plasma SH levels were decreased. No changes were observed in the other measured parameters of the patient group compared with the controls. These data suggest the possible involvement of nitric oxide (NO) together with reactive oxygen substances (ROS) in the pathogenesis of BD.展开更多
Yields of rice (<em>Oryza sativa</em> L.) respond to complex interactions between the genotype and the environment;rice has the particularity of being a semi-aquatic crop, and as a result of this, it prese...Yields of rice (<em>Oryza sativa</em> L.) respond to complex interactions between the genotype and the environment;rice has the particularity of being a semi-aquatic crop, and as a result of this, it presents lower adaptation to the limiting water content of soil and is extremely sensitive to stress from drought;therefore, it is the most important limiting factor in rice production. The objective of this study was to research the physiological response of rice genotypes to water stress. The grain yield and its components, leaf area and transpiration efficiency under irrigation (I) and drought (D) were evaluated in eight advanced lines from the nursery of the Latin American Fund for Irrigated Rice (Fondo Latinoamericano para Arroz de Riego, FLAR) and a control variety of rice. The experiment was established in Campeche, in the 2015 autumn-winter cycle. The grain yield and its components, as well as the leaf area were greater under irrigation conditions than under drought. The transpiration from the water stress was reduced and the plants under drought increased their transpiration efficiency. The genotypes P-V 2006 and P-V 2009 were identified, with genealogies FL05392-3P-12-2P-2P-M and FL08224-3P-2-1P-3P-M, respectively, as the most outstanding compared to the rest of the genotypes in the grain yield and its components, leaf area, total root biomass, and transpiration efficiency.展开更多
AIM: Nigella sativa L.(Ranunculaceae) is considered as a therapeutic plant-based medicine for liver damage. In this study, the aim was to study the effect of Nigella sativa oil(NSO) pretreatment on ethanol-induced hep...AIM: Nigella sativa L.(Ranunculaceae) is considered as a therapeutic plant-based medicine for liver damage. In this study, the aim was to study the effect of Nigella sativa oil(NSO) pretreatment on ethanol-induced hepatotoxicity in rats. METHOD: Rats were given Nigella sativa oil at doses of 2.5 and 5.0 mL·kg-1, orally for 3 weeks, followed by oral ethanol(EtOH) administration(5 g·kg-1) every 12 h three times(binge model). RESULTS: Binge ethanol application caused significant increases in plasma transaminase activities and hepatic triglyceride and malondialdehyde(MDA) levels. It decreased hepatic glutathione(GSH) levels, but did not change vitamins E and vitamin C levels and antioxidant enzyme activities. NSO(5.0 mL·kg-1) pretreatment significantly decreased plasma transaminase activities, hepatic MDA, and triglyceride levels together with amelioration in hepatic histopathological findings. CONCLUSION: NSO pretreatment may be effective in protecting oxidative stress-induced hepatotoxicity after ethanol administration.展开更多
基金A Grant Obtained from Marmara University School of Medicine, SAG-TUS-300505-0214
文摘AIM: To determine the role of inflammatory cytokines and reactive oxygen species (ROS) in childhood reflux esophagitis. METHODS: A total of 59 subjects who had complaints suggesting GERD underwent esophagogastroduoden oscopy. Endoscopic and histopathologic diagnosis of reflux esophagitis was established by Savary-Miller and Vandenplas grading systems, respectively. Esophageal biopsy specimens were taken from the esophagus 20% proximal above the esophagogastric junction for conventional histopathological examination and the measurements of ROS and cytokine levels. ROS were measured by chemiluminescence, whereas IL-8 and MCP-1 levels were determined with quantitative immunometric ELISA on esophageal tissue. Esophagealtissue ROS, IL-8 and MCP-1 levels were compared among groups with and without endoscopic/histo- pathologic esophagitis. RESULTS: Of 59 patients 28 (47.5%) had normal esophagus whereas 31 (52.5%) had endoscopic esophagitis. In histopathological evaluation, almost 73% of the cases had mild and 6.8% had moderate degree of esophagitis. When ROS and chemokine levels were compared among groups with and without endoscopic esophagitis, statistical difference could not be found between patients with and without esophagitis. Although the levels of ROS, IL-8 and MCP-1 were found to be higher in the group with histopathological reflux esophagitis, this difference was not statistically significant. CONCLUSION: These results suggest that the grade of esophagitis is usually mild or moderate during childhood and factors apart from ROS, IL-8 and MCP-1 may be involved in the pathogenesis of reflux esophagitis in children.
文摘Plant-derived compounds have been recognized by the feed industry as important supplements for livestock welfare and health. In this context, <i>Citrus aurantium</i> L. extract and <i>Origanum vulgare</i> L. essential oil have been demonstrated to have strong anti-inflammatory and antioxidant effects on animals. Being the composition of plant-derived extracts extremely influenced by the environmental and growing conditions of the plants, quality control is necessary in terms of the concentration of the active compounds to assure the reproducibility of natural feed additives. The present work aimed at the validation of the extraction procedure from feed additives of Hesperidin (HES) and Carvacrol (CAR), the main active compounds of <i>Citrus aurantium</i> and <i>Origanum vulgare</i> extracts. Then, the quantification method of both the analytes was developed and validated by reversed high-performance liquid chromatography coupled with a UV detector. The validated method was tested on premixtures and final feed additives supplied by a local feed factory to supervise the production chain. The extraction method with methanol resulted to be efficient and highly reproducible, with recovery higher than 90% for both the analytes. The chromatographic method has been demonstrated to be accurate, precise (relative standard deviation percent lower than 2.06%), and linear in the tested range concentrations, with regression coefficients equal to 0.995 and 0.999 for HES and CAR respectively. The method demonstrated that the feed additives prepared by the factory by diluting the premixtures were less concentrated than what was declared on the label.
文摘Somalia Mogadishu-Turkey Training and Research Hospital is only powered by diesel generator currently.In this paper,the energy demand of this hospital is supplied by determining the optimum hybrid power renewable generating system.Therefore,numerous hybrid renewable power generating systems including the components like diesel generator,wind turbine,photovoltaic(PV)and battery are considered in different configurations.Eventually,they are technically,environmentally and economically analyzed by using the well-known HOMER software.Furthermore,a sensitivity analysis is also performed considering variations in three important parameters,namely average wind speed,current diesel price and also solar radiation.According to the results,the optimal system is the standalone Wind/Diesel/Battery hybrid renewable energy system(HRES)with the configuration of 1,000 kW wind turbine,350 kW diesel generator,250 kW power converters and 750 batteries.Additionally,this system has the net present cost of$5,056,700 as well as the cost of energy as$0.191/kWh.Lastly,it is clearly occurred that the Wind/Diesel/Battery HRES is eco-friendlier than other HRESs.
文摘We report a case of a 7- year- old girl with hyperimmunoglobulin- E syndrome presenting with widespread oral papillomas which were tested for human papilloma virus DNA and had shown to be at high/intermediate risk group formalignancy. She had elevated levels of IgE, recurrent sinopulmonary infections, atopic- like dermatitis, peripheral eosinophilia and defective neutrophil chemotaxis. Interferon alfa 2b therapy and chemoprophylaxis with sulfamethoxazole- trimethoprim was given. Although the papillomas partially improved with the treatment, sinopulmonary infections continue to occur.
文摘Plasma nitrotyrosine and nitrite/nitrate levels as markers of nitrosative stress and plasma malondialdehyde (MDA) and protein carbonyl as markers of oxidative stress were determined in patients with Behc.et disease (BD). To evaluate the balance between oxidant and antioxidant systems in these patients, we measurederythrocytelysateCuZnsuperoxidedismutase(CuZn SOD) activity, plasma sulfhydryl (SH) values and total antioxidant activity. We also determined levels of plasma C- reactive protein (CRP), a key marker of inflammation, and compared them with those of healthy subjects. We found plasma nitro- tyrosine levels of BD patients to be increased, indicating that nitrosative stress may occur in these patients. Plasma MDA and CRP levels in BD patients were found to be significantly higher than those in control group. However, plasma SH levels were decreased. No changes were observed in the other measured parameters of the patient group compared with the controls. These data suggest the possible involvement of nitric oxide (NO) together with reactive oxygen substances (ROS) in the pathogenesis of BD.
文摘Yields of rice (<em>Oryza sativa</em> L.) respond to complex interactions between the genotype and the environment;rice has the particularity of being a semi-aquatic crop, and as a result of this, it presents lower adaptation to the limiting water content of soil and is extremely sensitive to stress from drought;therefore, it is the most important limiting factor in rice production. The objective of this study was to research the physiological response of rice genotypes to water stress. The grain yield and its components, leaf area and transpiration efficiency under irrigation (I) and drought (D) were evaluated in eight advanced lines from the nursery of the Latin American Fund for Irrigated Rice (Fondo Latinoamericano para Arroz de Riego, FLAR) and a control variety of rice. The experiment was established in Campeche, in the 2015 autumn-winter cycle. The grain yield and its components, as well as the leaf area were greater under irrigation conditions than under drought. The transpiration from the water stress was reduced and the plants under drought increased their transpiration efficiency. The genotypes P-V 2006 and P-V 2009 were identified, with genealogies FL05392-3P-12-2P-2P-M and FL08224-3P-2-1P-3P-M, respectively, as the most outstanding compared to the rest of the genotypes in the grain yield and its components, leaf area, total root biomass, and transpiration efficiency.
基金supported by the Research Fund of Istanbul University(No.6101)
文摘AIM: Nigella sativa L.(Ranunculaceae) is considered as a therapeutic plant-based medicine for liver damage. In this study, the aim was to study the effect of Nigella sativa oil(NSO) pretreatment on ethanol-induced hepatotoxicity in rats. METHOD: Rats were given Nigella sativa oil at doses of 2.5 and 5.0 mL·kg-1, orally for 3 weeks, followed by oral ethanol(EtOH) administration(5 g·kg-1) every 12 h three times(binge model). RESULTS: Binge ethanol application caused significant increases in plasma transaminase activities and hepatic triglyceride and malondialdehyde(MDA) levels. It decreased hepatic glutathione(GSH) levels, but did not change vitamins E and vitamin C levels and antioxidant enzyme activities. NSO(5.0 mL·kg-1) pretreatment significantly decreased plasma transaminase activities, hepatic MDA, and triglyceride levels together with amelioration in hepatic histopathological findings. CONCLUSION: NSO pretreatment may be effective in protecting oxidative stress-induced hepatotoxicity after ethanol administration.
