Soil science is an inherently diverse and multidisciplinary subject that cannot develop further without the continuous introduction and promotion of emerging technologies.One such technology that is widely used in bio...Soil science is an inherently diverse and multidisciplinary subject that cannot develop further without the continuous introduction and promotion of emerging technologies.One such technology that is widely used in biomedicine and similar research fields,microfluidics,poses significant benefits for soil research;however,this technology is still underutilized in the field.Microfluidics offers unparalleled opportunities in soil bacterial cultivation,observation,and manipulation when compared to conventional approaches to these tasks.This review focuses on the use of microfluidics for bacteria research and,where possible,pulls from examples in the literature where the technologies were used for soil related research.The review also provides commentary on the use of microfluidics for soil bacteria research and discusses the key challenges researchers face when implementing this technology.We believe that microfluidic chips and their associated auxiliary technologies provide a prime inroad into the future of soil science research.展开更多
Soil biogeochemical cycles and their interconnections play a critical role in regulating functions and services of environmental systems.However,the coupling of soil biogeochemical processes with their mediating micro...Soil biogeochemical cycles and their interconnections play a critical role in regulating functions and services of environmental systems.However,the coupling of soil biogeochemical processes with their mediating microbes remains poorly understood.Here,we identified key microbial taxa regulating soil biogeochemical processes by exploring biomarker genes and taxa of contigs assembled from metagenomes of forest soils collected along a latitudinal transect(18°N to 48°N)in eastern China.Among environmental and soil factors,soil pH was a sensitive indicator for functional gene composition and diversity.A function-taxon bipartite network inferred from metagenomic contigs identified the microbial taxa regulating coupled biogeochemical cycles between carbon and phosphorus,nitrogen and sulfur,and nitrogen and iron.Our results provide novel evidence for the coupling of soil biogeochemical cycles,identify key regulating microbes,and demonstrate the efficacy of a new approach to investigate the processes and microbial taxa regulating soil ecosystem functions.展开更多
基金supported by the Zhejiang Provincial Natural Science Foundation of China(LD19D060001)the National Natural Science Foundation of China(42090060).
文摘Soil science is an inherently diverse and multidisciplinary subject that cannot develop further without the continuous introduction and promotion of emerging technologies.One such technology that is widely used in biomedicine and similar research fields,microfluidics,poses significant benefits for soil research;however,this technology is still underutilized in the field.Microfluidics offers unparalleled opportunities in soil bacterial cultivation,observation,and manipulation when compared to conventional approaches to these tasks.This review focuses on the use of microfluidics for bacteria research and,where possible,pulls from examples in the literature where the technologies were used for soil related research.The review also provides commentary on the use of microfluidics for soil bacteria research and discusses the key challenges researchers face when implementing this technology.We believe that microfluidic chips and their associated auxiliary technologies provide a prime inroad into the future of soil science research.
基金supported by the National Natural Science Foundation of China(41721001,41991334)111 Project(B17039)Zhejiang Natural Science Foundation(LD19D060001).
文摘Soil biogeochemical cycles and their interconnections play a critical role in regulating functions and services of environmental systems.However,the coupling of soil biogeochemical processes with their mediating microbes remains poorly understood.Here,we identified key microbial taxa regulating soil biogeochemical processes by exploring biomarker genes and taxa of contigs assembled from metagenomes of forest soils collected along a latitudinal transect(18°N to 48°N)in eastern China.Among environmental and soil factors,soil pH was a sensitive indicator for functional gene composition and diversity.A function-taxon bipartite network inferred from metagenomic contigs identified the microbial taxa regulating coupled biogeochemical cycles between carbon and phosphorus,nitrogen and sulfur,and nitrogen and iron.Our results provide novel evidence for the coupling of soil biogeochemical cycles,identify key regulating microbes,and demonstrate the efficacy of a new approach to investigate the processes and microbial taxa regulating soil ecosystem functions.
