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Tonglian Decoction(通莲汤)Arrests the Cell Cycle in S-phase by Targeting the Nuclear Factor-Kappa B Signal Pathway in Esophageal Carcinoma Eca109 Cells 被引量:3
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作者 贾永森 胡雪琴 +3 位作者 李继安 Andras Szasz Gabriella Hegyi 韩炳生 《Chinese Journal of Integrative Medicine》 SCIE CAS CSCD 2016年第5期384-389,共6页
Objective: To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction (通莲汤, TLD) on esophageal carcinoma Eca109 cells. Methods: Ecal09 cells were treated with TLD and its separated for... Objective: To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction (通莲汤, TLD) on esophageal carcinoma Eca109 cells. Methods: Ecal09 cells were treated with TLD and its separated formulae, including the clearing-heat and detoxification formula (Q), activating-blood and promoting-qi formula (H) and nourishing-yin and blood formula (Z). Cell proliferation was measured using the 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, cell morphology was observed using a microscope, the cell cycle was measured using flow cytometry and the activity of the nuclear factor-kappa B (NF- K B) signal pathway was detected by Western blot. Results: The half maximal inhibitory concentrations of TLD, Q and H were 386, 771 and 729 rag/L, respectively. TLD, Q and H significantly inhibited cell proliferation, with 69.43%, 60.84% and 61.90% of treated cells in the G1 phase of the cell cycle. The percentage of cells in S phase increased significantly after treatment with TLD, Q, and H compared with the control group (P〈0.05), and TLD showed the strongest effect. Z had no influence on the cell cycle compared with the control group (P〉0.05). Western blot detection indicated slight differences in the inhibition of the NF- K B pathway by the different formulae. TLD formula strongly inhibited IKK β, NF- K B, interleukin-6 and tumor necrosis factor-α expression compared with the control group. Conclusions: TLD inhibited Eca109 cell proliferation by arresting cells in S phase. The possible mechanism might be related to inhibiting the NF- K B transduction cascade. The combination of the herbs found in the three separate formulae, H, Q and Z, work synergistically in TLD to produce the inhibitory effects of TLD treatment on Eca109 proliferation. 展开更多
关键词 esophageal carcinoma Eca109 cells Tonglian Decoction proliferation cell cycle nuclear factor-kappa B Chinese medicine
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