Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects...Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects of Daji （Cirsiumjaponicum） polysaccharide extracts （CJP） against hydrogen peroxide （H2O2） shock in rat H9c2 myocardial cells. First,CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquidchromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cellinjury model. The 3- （4,5- dimethylthiazol- 2-yl）-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatmentsignificantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. Inaddition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP onmyocardial cells. Therefore, we conclude that polysaccharide extracts of Daji （Cirsium japonicum） protect rat H9c2myocardial cells from oxidative stress induced by H2O2.展开更多
Adolescent idiopathic scoliosis （AIS） is one of the most prevalent diseases among teenagers, with an incidence rate of 1-3% worldwide, and at least 0.02% of patients require surgical treatment. The ＂gold standard＂...Adolescent idiopathic scoliosis （AIS） is one of the most prevalent diseases among teenagers, with an incidence rate of 1-3% worldwide, and at least 0.02% of patients require surgical treatment. The ＂gold standard＂ procedure is instrumentation and fusion of 10 or more vertebrae with forceful correction of the deformity.Although autogenous bone grafts are the ＂gold standard＂ for spine fusion, significant progress has been made in discovering bone graft alternatives, including freeze-dried allograft, demineralized bone matrix （DBM）, triosite ceramics, and bone marrow aspirate （BMA）, which have been used as substitutes for iliac crest in AIS surgery.展开更多
文摘Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects of Daji （Cirsiumjaponicum） polysaccharide extracts （CJP） against hydrogen peroxide （H2O2） shock in rat H9c2 myocardial cells. First,CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquidchromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cellinjury model. The 3- （4,5- dimethylthiazol- 2-yl）-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatmentsignificantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. Inaddition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP onmyocardial cells. Therefore, we conclude that polysaccharide extracts of Daji （Cirsium japonicum） protect rat H9c2myocardial cells from oxidative stress induced by H2O2.
基金grants from the National Natural Science Foundation of China，Shanghai Natural Science Foundation，Youth Project of Shanghai Municipal Health and Family Planning Commission
文摘Adolescent idiopathic scoliosis （AIS） is one of the most prevalent diseases among teenagers, with an incidence rate of 1-3% worldwide, and at least 0.02% of patients require surgical treatment. The ＂gold standard＂ procedure is instrumentation and fusion of 10 or more vertebrae with forceful correction of the deformity.Although autogenous bone grafts are the ＂gold standard＂ for spine fusion, significant progress has been made in discovering bone graft alternatives, including freeze-dried allograft, demineralized bone matrix （DBM）, triosite ceramics, and bone marrow aspirate （BMA）, which have been used as substitutes for iliac crest in AIS surgery.
