Ischemic stroke causes long-term disability and results in motor impairments.Such impairments are associated with structural changes in the neuromuscular junction(NMJ),including detailed morphology and three-dimension...Ischemic stroke causes long-term disability and results in motor impairments.Such impairments are associated with structural changes in the neuromuscular junction(NMJ),including detailed morphology and three-dimensional(3D)distribution.However,previous studies only explored morphological changes of individual NMJs after stroke,which limits the understanding of their role in post-stroke motor impairment.Here,we examine 3D distributions and detailed morphology of NMJs in entire mouse muscles after unilateral and bilateral strokes induced by photothrombosis.The results show that 3D distributions and numbers of NMJs do not change after stroke,and severe unilateral stroke causes similar levels of NMJ fragmentation and area enlargement to bilateral stroke.This research provides structural data,deepening the understanding of neuromuscular pathophysiology after stroke.展开更多
Modern optical imaging techniques provide powerful tools for observing cortical structure and functions at high resolutions.Various skull windows have been established for different applications of cortical imaging,an...Modern optical imaging techniques provide powerful tools for observing cortical structure and functions at high resolutions.Various skull windows have been established for different applications of cortical imaging,and each has its advantages and limitations.Most critical of the limitations,none of the current skull windows is suitable for observing the responses to some acute craniocerebral injuries on a large scale and at high resolution.Here,we developed a“Through-Intact-Skull(TIS)window”that enables the observation of an immune response on a bilateral cortical scale and at single-cell resolution after traumatic brain injury without affecting the pathological environment of the brain.The TIS window also has the advantages of craniotomy-freeness,centimeter-field of view,synaptic resolution,large imaging depth,long-term observation capability,and suitability for awake mice.Therefore,the TIS window is a promising new approach for intravital cortical microscopy in basic research in neuroscience.展开更多
基金supported by the National Natural Science Foundation of China(No.61860206009)the Key Research and Development Project of Hubei Province(No.2022BCA023)the Innovation Fund of WNLO.
文摘Ischemic stroke causes long-term disability and results in motor impairments.Such impairments are associated with structural changes in the neuromuscular junction(NMJ),including detailed morphology and three-dimensional(3D)distribution.However,previous studies only explored morphological changes of individual NMJs after stroke,which limits the understanding of their role in post-stroke motor impairment.Here,we examine 3D distributions and detailed morphology of NMJs in entire mouse muscles after unilateral and bilateral strokes induced by photothrombosis.The results show that 3D distributions and numbers of NMJs do not change after stroke,and severe unilateral stroke causes similar levels of NMJ fragmentation and area enlargement to bilateral stroke.This research provides structural data,deepening the understanding of neuromuscular pathophysiology after stroke.
基金National Natural Science Foundation of China(NSFC)(Grant Nos.61860206009,81870934,82001877,61975172,61735016,91632105,81961128029,81961138015)National Key Research and Development Program of China(2017YFA0700501)+2 种基金China Postdoctoral Science Foundation-funded project(Nos.BX20190131,2019M662633)Innovation Project of Optics Valley Laboratory(Grant No.OVL2021BG011)Funding from the Innovation Fund of WNLO,and Fundamental Research Funds for the Central Universities(Nos.2020-KYY-511108-0007,2019QNA5001).
文摘Modern optical imaging techniques provide powerful tools for observing cortical structure and functions at high resolutions.Various skull windows have been established for different applications of cortical imaging,and each has its advantages and limitations.Most critical of the limitations,none of the current skull windows is suitable for observing the responses to some acute craniocerebral injuries on a large scale and at high resolution.Here,we developed a“Through-Intact-Skull(TIS)window”that enables the observation of an immune response on a bilateral cortical scale and at single-cell resolution after traumatic brain injury without affecting the pathological environment of the brain.The TIS window also has the advantages of craniotomy-freeness,centimeter-field of view,synaptic resolution,large imaging depth,long-term observation capability,and suitability for awake mice.Therefore,the TIS window is a promising new approach for intravital cortical microscopy in basic research in neuroscience.
