Human umbilical mesenchymal stem cells from Wharton＇s jelly of the umbilical cord were induced to differentiate into oligodendrocyte precursor-like cells in vitro. Oligodendrocyte precursor cells were transplanted in...Human umbilical mesenchymal stem cells from Wharton＇s jelly of the umbilical cord were induced to differentiate into oligodendrocyte precursor-like cells in vitro. Oligodendrocyte precursor cells were transplanted into contused rat spinal cords. Immunofluorescence double staining indicated that transplanted cells survived in injured spinal cord, and differentiated into mature and immature oligodendrocyte precursor cells. Biotinylated dextran amine tracing results showed that cell transplantation promoted a higher density of the corticospinal tract in the central and caudal parts of the injured spinal cord. Luxol fast blue and toluidine blue staining showed that the volume of residual myelin was significantly increased at 1 and 2 mm rostral and caudal to the lesion epicenter after cell transplantation. Furthermore, immunofluorescence staining verified that the newly regenerated myelin sheath was derived from the central nervous system. Basso, Beattie and Bresnahan testing showed an evident behavioral recovery. These results suggest that human umbilical mesenchymal stem cell-derived oligodendrocyte precursor cells promote the regeneration of spinal axons and myelin sheaths.展开更多
Background： Most patients with epilepsy want to learn as much as possible about the disease, and many have turned to the internet for information. Patients are likely to use information obtained from the internet to ...Background： Most patients with epilepsy want to learn as much as possible about the disease, and many have turned to the internet for information. Patients are likely to use information obtained from the internet to control their epilepsy, but little is known about the accuracy of this information. In this survey, we have assessed the feasibility and usability ofinternet-based interventions for the treatment of epilepsy. Methods： Data were collected from an internet search. Different search terms were used to obtain general information on epilepsy together with information about medication, types of epilepsy, treatment, women＇s health, and other information. The accuracy of the information was evaluated by a group of experts. Results： A total of 1320 web pages were assessed. The majority were websites related to health. A large number （80.2%） of web pages contained content related to the search term. A significant number of web pages 450/1058 （42.5%） claimed to provide information from a credible source： however, only 206/1058 （19.5%） of the information was accurate and complete; 326/1058 （30.8%） was accurate but incomplete; 328/1058 （31.0%） was correct but nonstandard, and 198/1058 （18.8%） was inaccurate. The authenticity of the information was not significantly different between the two search engines （x^2 = 0.009, P - 0.924）. No significant difference was observed in the information obtained from a specialist or nonspecialist source （x^2 = 7.538, P- 0.057）. There was also no correlation between the quality of the information and the priority （x^2 = 6.880, P = 0,076）. Conclusions： Searching for information about epilepsy on the internet is convenient, but the information provided is not reliable. Too much information is inaccurate or for advertisement purposes, and it is difficult for patients to find the useful information. Turning to the internet for medical knowledge may be harmful. Physicians should be aware that their patients may search for information on the internet and guide them to safe, reputable websites.展开更多
EPilepsy is a common neurological disorder among all age groups and socioeconomic classes. Epilepsy surgery has been demonstrated to be a safe and effective treatment for drug-refractory epilepsy.Despite this success...EPilepsy is a common neurological disorder among all age groups and socioeconomic classes. Epilepsy surgery has been demonstrated to be a safe and effective treatment for drug-refractory epilepsy.Despite this success, many potential surgical candidates refuse surgery. On average, there is a 20-year delay between the diagnosis of epilepsy and surgical treatment,1 suggesting that epilepsy surgery is underutilized. The prevalence of active epilepsy is even higher in developing countries due to the limited availability and high cost of medication. To date, however, few studies have addressed the reasons for delayed surgical treatment in developing countries such as China. To address these issues, we conducted a cross- sectional survey to document potential barriers limiting patients access to the surgical treatment of epilepsy.展开更多
基金supported by the National Natural Science Foundation of China, No. 81100916, 30400464,81271316the Postdoctoral Science Foundation of China,No. 201104901907
文摘Human umbilical mesenchymal stem cells from Wharton＇s jelly of the umbilical cord were induced to differentiate into oligodendrocyte precursor-like cells in vitro. Oligodendrocyte precursor cells were transplanted into contused rat spinal cords. Immunofluorescence double staining indicated that transplanted cells survived in injured spinal cord, and differentiated into mature and immature oligodendrocyte precursor cells. Biotinylated dextran amine tracing results showed that cell transplantation promoted a higher density of the corticospinal tract in the central and caudal parts of the injured spinal cord. Luxol fast blue and toluidine blue staining showed that the volume of residual myelin was significantly increased at 1 and 2 mm rostral and caudal to the lesion epicenter after cell transplantation. Furthermore, immunofluorescence staining verified that the newly regenerated myelin sheath was derived from the central nervous system. Basso, Beattie and Bresnahan testing showed an evident behavioral recovery. These results suggest that human umbilical mesenchymal stem cell-derived oligodendrocyte precursor cells promote the regeneration of spinal axons and myelin sheaths.
文摘Background： Most patients with epilepsy want to learn as much as possible about the disease, and many have turned to the internet for information. Patients are likely to use information obtained from the internet to control their epilepsy, but little is known about the accuracy of this information. In this survey, we have assessed the feasibility and usability ofinternet-based interventions for the treatment of epilepsy. Methods： Data were collected from an internet search. Different search terms were used to obtain general information on epilepsy together with information about medication, types of epilepsy, treatment, women＇s health, and other information. The accuracy of the information was evaluated by a group of experts. Results： A total of 1320 web pages were assessed. The majority were websites related to health. A large number （80.2%） of web pages contained content related to the search term. A significant number of web pages 450/1058 （42.5%） claimed to provide information from a credible source： however, only 206/1058 （19.5%） of the information was accurate and complete; 326/1058 （30.8%） was accurate but incomplete; 328/1058 （31.0%） was correct but nonstandard, and 198/1058 （18.8%） was inaccurate. The authenticity of the information was not significantly different between the two search engines （x^2 = 0.009, P - 0.924）. No significant difference was observed in the information obtained from a specialist or nonspecialist source （x^2 = 7.538, P- 0.057）. There was also no correlation between the quality of the information and the priority （x^2 = 6.880, P = 0,076）. Conclusions： Searching for information about epilepsy on the internet is convenient, but the information provided is not reliable. Too much information is inaccurate or for advertisement purposes, and it is difficult for patients to find the useful information. Turning to the internet for medical knowledge may be harmful. Physicians should be aware that their patients may search for information on the internet and guide them to safe, reputable websites.
文摘EPilepsy is a common neurological disorder among all age groups and socioeconomic classes. Epilepsy surgery has been demonstrated to be a safe and effective treatment for drug-refractory epilepsy.Despite this success, many potential surgical candidates refuse surgery. On average, there is a 20-year delay between the diagnosis of epilepsy and surgical treatment,1 suggesting that epilepsy surgery is underutilized. The prevalence of active epilepsy is even higher in developing countries due to the limited availability and high cost of medication. To date, however, few studies have addressed the reasons for delayed surgical treatment in developing countries such as China. To address these issues, we conducted a cross- sectional survey to document potential barriers limiting patients access to the surgical treatment of epilepsy.
