One patient of hepatic veno-occlusive disease with an increased cancer antigen-125 expression caused by misuse of Sedum aizoon

Nowadays hepatic veno-occlusive disease is mainly caused by Sedum aizoon in China,and its prognosis dependents on the dosage and courses of the Sedum aizoon treatment but lacks other objective indicators.There are a lot relationships between CA125 level and liver cirrhosis,this case had a obvious increased CA125 level in the serum,hydrothorax and ascites,following by the liver cirrhosis in a short time,and then died of upper gastrointestinal bleeding.By now we guess that CA125 level could forecast the liver cirrhosis following by hepatic veno-occlusive,which will become the prognosis of the hepatic veno-occlusive.

Paying Attention to Special Populations:1 Case of COVID-19 Asymptomatic Infection with Positive Nucleic Acid and Negative Antibodies in Puerpera

As a newly emerged disease,COVID-19's in-depth understanding is still being explored.And also as the epidemic changes,various new situations will be encountered.Atypical patients have brought difficulties to our prevention and control of this disease.Among them,asymptomatic patients are a special category,and puerperae are a special population.What happens when a puerpera is infected with asymptomatic COVID-19?This study describes 1 case of COVID-19 asymptomatic infection with positive nucleic acid and negative antibodies in puerpera.The aim is to remind clinicians to take special cases seriously,such as puerperae and pregnant women,AIDS,and other immunocompromised patients.It may not be enough to rely solely on antibody detection,but more than two repeated nucleic acid tests are necessary,so that no suspicious patient can be easily let off.

Identification and Validation of an Immune-related Prognostic Signature for Hepatocellular Carcinoma

Background and Aims:The immune system plays vital roles in hepatocellular carcinoma(HCC)initiation and progression.The present study aimed to construct an immune-gene related prognostic signature(IRPS)for predicting the prognosis of HCC patients.Methods:Gene expression data were retrieved from The Cancer Genome Atlas database.The IRPS was established via least absolute shrinkage and selection operator(LASSO)and multivariate Cox regression analysis.The prognostic values of the IRPS were further validated using the International Cancer Genome Consortium(ICGC)dataset.Results:A total of 62 genes were identified as candidate immune-related prognostic genes.According to the results of Lasso and multivariate Cox regression analysis,we established an IRPS and confirmed its stability and reliability in the ICGC dataset.The IRPS was significantly associated with advanced clinicopathological characteristics.Both Cox regression analyses revealed that the IRPS could be independent risk factors influencing prognosis of HCC patients.The relationships between the IRPS and infiltration of immune cells demonstrated that the IRPS was associated with immune cell infiltration.Furthermore,a nomogram was constructed to estimate the survival probability of HCC patients.Conclusions:The IRPS was effective for predicting prognosis of HCC patients,which might serve as novel prognostic and therapeutic biomarkers for HCC.

Sijunzi Decoction Inhibits Stemness by Suppressingβ-Catenin Transcriptional Activity in Gastric Cancer Cells

Objective:To investigate a previously uncharacterized function of Sijunzi Decoction(SJZD)in inhibition of gastric cancer stem cells(GCSCs).Methods:MKN74 and MKN45,two CD44 positive gastric cancer cell lines with stem cell properties were used.The cells were divided into 2 groups.Treatment group was treated with SJZD(1-5 mg/mL)for indicated time(48 h-14 days).The control group was treated with equal volume of phosphate buffered saline.Cell Counting Assay Kit-8 were used to measure cell viability.Spheroid colony formation and GCSCs marker expression were performed to determine GCSCs stemness.Cell fractionation and chromatin immunoprecipitation assays were used to assess the distribution and DNA-binding activity of β-catenin after SJZD treatment,respectively.Results:SJZD treatment repressed cell growth and induced apoptosis in MKN74 and MKN45 cell lines(P<0.05).Moreover,SJZD dramatically inhibited formation of spheroid colony and expression of GCSC markers in GC cells(P<0.05).Mechanistically,SJZD reduced nuclear accumulation and DNA binding activity ofβ-catenin(P<0.05),the key regulator for maintaining CSC stemness.Conclusion:SJZD inhibits GCSCs by attenuating the transcriptional activity of β-catenin.