Radix Glehniae extract inhibits the migration and invasion abilities of lung cancer cells

Objective: To investigate the effect of Radix Glehniae on the migration and invasion abilities of lung cancer cells, and to explore the underlying mechanism. Method: Normal human bronchial cell line 16 HBE and lung cancer cell line SK-MES-1 were cultured in vitro. Radix Glehniae extract was prepared to treat the two kinds of cells. The proliferation ability of 16 HBE cells was determined by CCK-8 assay. The migration and invasion abilities of SK-MES-1 cells were determined by Transwell and Matrigel assays, respectively. The expression of TIMP2 m RNA in SK-MES-1 cells was detected by fluorescence quantitative PCR(q-PCR). The secretion level of TIMP2 protein by SK-MES-1 cells was measured by ELISA experiment. Results: Radix Glehniae extract caused no toxic effects on 16 HBE cells at the concentrations of 1 mg/ml to 15 mg/ml. Radix Glehniae extract(from 5 mg/ml to 15 mg/ml) significantly inhibited SK-MES-1 cell migration and invasion abilities, as well as upregulated TIMP2 m RNA expression and enhanced TIMP2 secretion by SK-MES-1 cells. Conclusion: Radix Glehniae can inhibit the migration and invasion behaviors of lung cancer cells by upregulating TIMP2 expression and secretion.

Characteristics of cancer susceptibility genes mutations in 282 patients with gastric adenocarcinoma

Objective:To reveal the distribution signature of cancer susceptibility genes in patients with gastric adenocarcinoma,offering a diagnostic and prognostic surrogate for disease risk management and therapeutic decisions.Methods:A total of 282 patients with gastric adenocarcinoma(182 males and 100 females)were enrolled in this study,with peripheral blood genomic DNA extracted.Mutations of 69 canonical cancer susceptibility genes or presumably tumor-related genes were analyzed by targeted capture-based high-throughput sequencing.Candidate mutations were particularly selected for discussion on tumor pathogenesis according to the American College of Medical Genetics and Genomics(ACMG)guidelines.Results:In this study,7.1%(20/282)of patients with gastric adenocarcinoma were found to harbor mutations of canonical or presumable cancer susceptibility genes.The detection rate in male patients(3.8%,7/182)was significantly lower than that in female patients(13%,13/100)(P=0.004).The most recurrent mutations were in AT mutated(ATM)(1.1%,3/282),followed by BRCA1,BRIP1 and RAD51D,all showed a detection rate of 0.7%(2/282).Mutations in three genes associated with hereditary gastric cancer syndromes were detected,namely,PMS2 and EP CAM associated with Lynch syndrome and CDH1 associated with hereditary di ffuse gastric cancer.The detection frequencies were all 0.4%(1/282).Notwithstanding no significant difference observed,the age of patients with pathogenic mutations or likely pathogenic mutations is slightly younger than that of non-carriers(median age:58.5 vs.60.5 years old),while the age of patients with ATM mutations was the youngest overall(median age:49.3 years old).Conclusions:Our study shed more light on the distribution signature and pathogenesis of mutations in gastric cancer susceptibility genes,and found the detection rate of pathogenic and likely pathogenic mutations in male patients was significandy lower than that in female patients.Some known and unidentified mutations were found in gastric cancer,which allowed us to gain more insight into the hereditary gastric cancer syndromes from the molecular perspective.

Xanthii Fructus Inhibits Malignant Behaviors of Lung Cancer Cells by Changing the Expression of Small RNAs

Objective: To investigate the influence of Xanthii Fructuson the expression of small non-coding RNAs(snc RNA) and the malignant behaviors of lung cancer cells. Method: A549 human lung cancer cells were cultured and treated with different concentrations(5-15 mg/ml) of the ethanol extract of Xanthii Fructus. The expression of mi R-21 and pi RNA55490 was analyzed by Realtime PCR. The proliferation capacity was detected by CCK-8 assay. The anchorage-independent growth capacity was measured by soft agar colony formation experiment. The cell invasion capacity was determined by Matrigel assay. Results: The Xanthii Fructus alcohol extract down-regulated the expression of the oncogenic snc RNAmi R-21, and up-regulated the expression of the tumor suppressive snc RNA pi RNA55490. At the functional level, proliferation, anchorage-independent growth, and invasion capacities of the lung cancer cells were strongly inhibited by the extract treatment. Conclusion: The Chinese herb Xanthii Fructus inhibits the malignant behaviors of lung cancer cells, and the inhibition is largely associated with its ability to change the expression of snc RNAs.

A New Technique for The Therapy of Complicated Deep Tissue Infection(with 4 Cases)

Objective: The objective of this study was to discuss the effect of double perfusion cannula accompanied with low negative pressure drainage in the treatment of complexity of abdominopelvic and perineal infections. Methods: The technology of the double perfusion cannula accompanied with low negative pressure drainage was used to treat complexity of abdominopelvic and perineal infections. Results: Double perfusion cannula accompanied with low negative pressure drainage can be applied to the treatment of complexity of abdominopelvic and perineal infections. It has an obvious effect on infection control and reduces recovery time. Conclusion: Double perfusion cannula accompanied with low negative pressure drainage has a good effect on complexity of abdominopelvic and perineal infections; it can be used in wider surgical fields to prevent infections.