Molecular imprinting technology has a great potential to be used in protein separation and purification. In this work, lysozyme imprinted polyacrylamide gel was prepared with silica particles as a sacrificial template...Molecular imprinting technology has a great potential to be used in protein separation and purification. In this work, lysozyme imprinted polyacrylamide gel was prepared with silica particles as a sacrificial template to generate macro-porosity for fast adsorption. The adsorption equilibrium time and adsorption capacity were 9 h and 56 mg/g respectively, which was 2 h less and 2.3-fold more than polymers without the sacrificial template. In order to test molecular imprinting polymers (MIPs) ' selectivity, bovine serum albumin (BSA) was chosen as interferent for binary adsorption tests. In addition, the adsorption selectivity was further investigated using different molar ratios of lysozyme to BSA with fixed total concentration of proteins, as well as using various total concentra- tions of proteins with an equimolar ratio of lysozyme to BSA. It has been proven that the total con- centration of proteins should be larger than 1.5 × 10^-7 mol/mL, when the molar ratio of BSA to Lyz is 1: 1, in order to effectively separate Lyz from the binary protein mixture. The macro-porous lyso- zyme molecularly imprinted polymers have less adsorption time, larger adsorption capacity, and bet- ter imprinting effect.展开更多
Acanthopanax trifoliatus( L.) Merr is a common traditional Chinese herbal medicine in central and southern regions of China. It has high medicinal and edible value and mainly contains flavonoids,polysaccharides,saponi...Acanthopanax trifoliatus( L.) Merr is a common traditional Chinese herbal medicine in central and southern regions of China. It has high medicinal and edible value and mainly contains flavonoids,polysaccharides,saponins and volatile components. Its active extracts have antioxidant,anti-inflammatory,anti-fatigue,and analgesic effects. From the biological characteristics,effective chemical components,extraction methods,cultivation techniques,pharmacological effects,toxicological activities,and product development status,this paper reviewed in detail the research status of A. trifoliatus in China and abroad,to provide a scientific basis for in-depth research and development,and utilization of A. trifoliatus.展开更多
The widespread use of pesticides induces heavy adverse effects on human health,especially for the pregnant women and the newborns.In this study,a screening method has been developed for the determination of multi-pest...The widespread use of pesticides induces heavy adverse effects on human health,especially for the pregnant women and the newborns.In this study,a screening method has been developed for the determination of multi-pesticides in maternal and umbilical cord sera.All pesticides in sera were collected using solid phase extraction(SPE),and analyzed by gas chromatography-quadrupole time of flight mass spectrometry(GC-QTOF MS).To set up the quality criteria,a database of 50 pesticides was created and the accurate masses of 3 up to 5 representative ions with their intensity ratios were included for each pesticide.In addition,a novel"identification points"(IPs)system relying on the accurate MS1 and MS2 spectra was used to interpret the data for each suspected pesticide.The methodology was then applied to a pair of maternal and umbilical cord sera.A total of six pesticide residues were screened out successfully.In conclusion,GC-QTOF MS combined with an accurate mass database seemed to be one of the most efficient tools for systematic pesticide analysis.展开更多
In this study, ion mobility separation coupled with tandem mass spectrometry (IM-tandem MS) was utilized to investigate the ionization behaviors of two amino acids including leucine and isoleucine. Under the electro...In this study, ion mobility separation coupled with tandem mass spectrometry (IM-tandem MS) was utilized to investigate the ionization behaviors of two amino acids including leucine and isoleucine. Under the electrospray ionization (ESI) mode, two protonation sites in each molecular sturcture caused two forms of protomer. One arose from the amino being protonated (amino-protomer) and the other from the carboxyl being protonated (car- boxyl-protomer). In the two-dimensional (drift time, m/z) spectrum, the protomers had the same mass, but the dis- tinguishable drift times and fragmentation patterns. For the characterization purpose, the theoretical collision cross section (CCS) values of the protomers were calculated and proven to be consistent with the experimental. Moreover, the quantified relationship between the amino acids and their protomers was evaluated. It showed that the abun- dance of the carboxyl-protomer was proportional to the concentration of the amino acid, whereas that of the amino- protomer did not have the same trend. Under the atmospheric pressure chemical ionization (APCI) mode, only the carboxyl-protomer was observed. In addition, the amino-protomer and the cluster ions observed under ESI were absent completely. The results demonstrate that the ionization mode impacts heavily on the ionization behaviors of leucine and isoleucine not only on the form of therir protomers but also On the quantified relationship.展开更多
Traditional Chinese Medicine(TCM) has been popular for thousand years in prevention and treatment of chronic diseases synergistically with Western medicine while producing mild healing effects and lower side effects. ...Traditional Chinese Medicine(TCM) has been popular for thousand years in prevention and treatment of chronic diseases synergistically with Western medicine while producing mild healing effects and lower side effects. Although many TCMs have been proven effective by modern pharmacological studies and clinical trials, their bioactive constituents and the remedial mechanisms are still not well understood. Researchers have made great efforts to explore the real theory of TCM for many years with different strategies. Development of high performance liquid chromatography(HPLC) and mass spectrometry within recent decade can provide scientists with robust technologies for disclosing the mysterious mask of TCM. In this paper, important innovations of HPLC and mass spectrometry are reviewed in the application of TCM analysis from single compound identification to metabolomic strategy.展开更多
文摘Molecular imprinting technology has a great potential to be used in protein separation and purification. In this work, lysozyme imprinted polyacrylamide gel was prepared with silica particles as a sacrificial template to generate macro-porosity for fast adsorption. The adsorption equilibrium time and adsorption capacity were 9 h and 56 mg/g respectively, which was 2 h less and 2.3-fold more than polymers without the sacrificial template. In order to test molecular imprinting polymers (MIPs) ' selectivity, bovine serum albumin (BSA) was chosen as interferent for binary adsorption tests. In addition, the adsorption selectivity was further investigated using different molar ratios of lysozyme to BSA with fixed total concentration of proteins, as well as using various total concentra- tions of proteins with an equimolar ratio of lysozyme to BSA. It has been proven that the total con- centration of proteins should be larger than 1.5 × 10^-7 mol/mL, when the molar ratio of BSA to Lyz is 1: 1, in order to effectively separate Lyz from the binary protein mixture. The macro-porous lyso- zyme molecularly imprinted polymers have less adsorption time, larger adsorption capacity, and bet- ter imprinting effect.
基金Supported by Joint Fund Project of Guizhou Provincial Department of Science and Technology(Qian Ke He LH Zi[2015]7716)Open Fund Project for Development Engineering Research Center of National Medicinal Plant Resources of Colleges and Universities in Guizhou Province(Qian Jiao He KY Zi[2014]227)
文摘Acanthopanax trifoliatus( L.) Merr is a common traditional Chinese herbal medicine in central and southern regions of China. It has high medicinal and edible value and mainly contains flavonoids,polysaccharides,saponins and volatile components. Its active extracts have antioxidant,anti-inflammatory,anti-fatigue,and analgesic effects. From the biological characteristics,effective chemical components,extraction methods,cultivation techniques,pharmacological effects,toxicological activities,and product development status,this paper reviewed in detail the research status of A. trifoliatus in China and abroad,to provide a scientific basis for in-depth research and development,and utilization of A. trifoliatus.
基金supported by the Science and Technology Commission of Shanghai Municipality(13ZR1448100)
文摘The widespread use of pesticides induces heavy adverse effects on human health,especially for the pregnant women and the newborns.In this study,a screening method has been developed for the determination of multi-pesticides in maternal and umbilical cord sera.All pesticides in sera were collected using solid phase extraction(SPE),and analyzed by gas chromatography-quadrupole time of flight mass spectrometry(GC-QTOF MS).To set up the quality criteria,a database of 50 pesticides was created and the accurate masses of 3 up to 5 representative ions with their intensity ratios were included for each pesticide.In addition,a novel"identification points"(IPs)system relying on the accurate MS1 and MS2 spectra was used to interpret the data for each suspected pesticide.The methodology was then applied to a pair of maternal and umbilical cord sera.A total of six pesticide residues were screened out successfully.In conclusion,GC-QTOF MS combined with an accurate mass database seemed to be one of the most efficient tools for systematic pesticide analysis.
文摘In this study, ion mobility separation coupled with tandem mass spectrometry (IM-tandem MS) was utilized to investigate the ionization behaviors of two amino acids including leucine and isoleucine. Under the electrospray ionization (ESI) mode, two protonation sites in each molecular sturcture caused two forms of protomer. One arose from the amino being protonated (amino-protomer) and the other from the carboxyl being protonated (car- boxyl-protomer). In the two-dimensional (drift time, m/z) spectrum, the protomers had the same mass, but the dis- tinguishable drift times and fragmentation patterns. For the characterization purpose, the theoretical collision cross section (CCS) values of the protomers were calculated and proven to be consistent with the experimental. Moreover, the quantified relationship between the amino acids and their protomers was evaluated. It showed that the abun- dance of the carboxyl-protomer was proportional to the concentration of the amino acid, whereas that of the amino- protomer did not have the same trend. Under the atmospheric pressure chemical ionization (APCI) mode, only the carboxyl-protomer was observed. In addition, the amino-protomer and the cluster ions observed under ESI were absent completely. The results demonstrate that the ionization mode impacts heavily on the ionization behaviors of leucine and isoleucine not only on the form of therir protomers but also On the quantified relationship.
文摘Traditional Chinese Medicine(TCM) has been popular for thousand years in prevention and treatment of chronic diseases synergistically with Western medicine while producing mild healing effects and lower side effects. Although many TCMs have been proven effective by modern pharmacological studies and clinical trials, their bioactive constituents and the remedial mechanisms are still not well understood. Researchers have made great efforts to explore the real theory of TCM for many years with different strategies. Development of high performance liquid chromatography(HPLC) and mass spectrometry within recent decade can provide scientists with robust technologies for disclosing the mysterious mask of TCM. In this paper, important innovations of HPLC and mass spectrometry are reviewed in the application of TCM analysis from single compound identification to metabolomic strategy.