Acid Lime (Citrus aurantifolia Swingle) is one of the important commercial fruits cultivated from terai to high hill of Nepal. It is an important source of vitamin “C” (ascorbic acid) for human nutrition. Amount of ...Acid Lime (Citrus aurantifolia Swingle) is one of the important commercial fruits cultivated from terai to high hill of Nepal. It is an important source of vitamin “C” (ascorbic acid) for human nutrition. Amount of juice, TSS (Total soluble solids), TA (Titrable acid) and vitamin C are the determining factors of quality of acid lime fruits which may vary according to bearing sides of the trees. The main objective of this study is, to determine the variation of fruit quality at different sides of the tree. Total of 15 bearing trees were selected randomly (5 trees per site) from three different agro ecological domain representing terai (1200 m asl) and samples were collected from the selected trees. Randomly ten fruits (from east, west, centre, north and south sides) were collected from each tree and analyzed for amount of vitamin C, TSS, TA and juice. Highest ascorbic acid 79.6 mg and 69.9 mg was observed in south side fruits whereas lowest 62.8 mg and 55.1 mg was observed in centre fruits in the high and mid hills zone respectively, but in terai, highest ascorbic acid 58.7 mg was observed in north side and lowest 41.8 mg was observed in centre. Highest amount of juice 43.9% was observed in south side fruits and lowest 36.6% in centre fruits, but amount of TSS 8.2% and TA 7.2% was observed in south side fruits and lowest 7.3% TSS and 7.0% TA was observed in centre fruits in high hills. In terai highest TSS 8.3% and TA 7.4% was observed in north side fruits and lowest TSS 7.3% and TA 6.7% was observed in centre fruits. Variation of TSS, TA percent and ascorbic acids was observed according to the agro ecological zone.展开更多
Acid lime (Citrus aurantifolia) fruits are cultivated in terai, mid hill and high hill districts of Nepal. It is cross-pollinated crop and has a high variation in fruit characters within the species. Fruit character i...Acid lime (Citrus aurantifolia) fruits are cultivated in terai, mid hill and high hill districts of Nepal. It is cross-pollinated crop and has a high variation in fruit characters within the species. Fruit character is the main basis of genotype selection. Study of fruit diversity is of utmost importance to select the elite genotypes for breeding and variety development program. Therefore, total of 620 fruit samples were collected and evaluated in the main production season of the year 2010 and 2011, from 62 bearing trees. Samples were collected from three agro-ecological domains, representing terai, mid hill and high hill areas of eastern Nepal. Elite landraces were selected on the basis of scoring fruit characters. High variation in vitamin C was observed between the terai (57.4 mg) and high hill (72.5 mg) samples. Amount of juice was 36.8% in mid hill and 44.1% in terai samples. Higher percent of juice and total soluble solids (TSS) observed in terai samples but tritable acidity (TA) and vitamin C was low as compare to high hills. There was significantly negative correlation found in fruit weight, fruit diameter, juice percent and seed number, whereas significantly positive correlation was in pulp and vitamin C, with altitude range. Total landraces were separated in five major cluster group. Variation in the level of similarity was observed among the cluster groups. The variability distance of fruit characters was highest in cluster group II (–17.45%) and lowest in cluster group V (58.5%). On the basis of scoring number of fruit characters, total four elite genotypes i.e. LD-49 from mid hills, LD-23 and LT-17 from higher hills and LM-44 from terai were selected for conservation, breeding and variety development purpose.展开更多
Acid lime (Citrus aurantifolia) is an important commercial fruits crop, cultivated in terai to high hills of Nepal. High variation of acid lime fruits are observed in existing landraces due to crossing within the othe...Acid lime (Citrus aurantifolia) is an important commercial fruits crop, cultivated in terai to high hills of Nepal. High variation of acid lime fruits are observed in existing landraces due to crossing within the other citrus species. Determination of genetic variation is important to the plant breeders for development of high yielding variety and hybrids. Therefore, an attempt has been made to study the genetic diversity of 62 acid lime landraces, collected from different altitudinal range in the eastern part of Nepal, using SSR markers. Twelve Simple Sequence Repeat (SSR) primer pairs were used to assess the genetic diversity of acid lime. The average genetic similarity level among the 62 accessions was 0.77, ranging from 0.54 to 1.0 and separated five major cluster groups. Total of 33 alleles were detected by eleven primer pairs and size of alleles ranged from 50 to 225. Average polymorphic information content (PIC) value was 0.50, whereas highest 0.75 and lowest 0.18 was observed in CAT01 and GT03 loci respectively. The results of the study clearly indicated that, SSR markers are highly polymorphic and more informative for the assessment of genetic diversity of acid lime landraces.展开更多
文摘Acid Lime (Citrus aurantifolia Swingle) is one of the important commercial fruits cultivated from terai to high hill of Nepal. It is an important source of vitamin “C” (ascorbic acid) for human nutrition. Amount of juice, TSS (Total soluble solids), TA (Titrable acid) and vitamin C are the determining factors of quality of acid lime fruits which may vary according to bearing sides of the trees. The main objective of this study is, to determine the variation of fruit quality at different sides of the tree. Total of 15 bearing trees were selected randomly (5 trees per site) from three different agro ecological domain representing terai (1200 m asl) and samples were collected from the selected trees. Randomly ten fruits (from east, west, centre, north and south sides) were collected from each tree and analyzed for amount of vitamin C, TSS, TA and juice. Highest ascorbic acid 79.6 mg and 69.9 mg was observed in south side fruits whereas lowest 62.8 mg and 55.1 mg was observed in centre fruits in the high and mid hills zone respectively, but in terai, highest ascorbic acid 58.7 mg was observed in north side and lowest 41.8 mg was observed in centre. Highest amount of juice 43.9% was observed in south side fruits and lowest 36.6% in centre fruits, but amount of TSS 8.2% and TA 7.2% was observed in south side fruits and lowest 7.3% TSS and 7.0% TA was observed in centre fruits in high hills. In terai highest TSS 8.3% and TA 7.4% was observed in north side fruits and lowest TSS 7.3% and TA 6.7% was observed in centre fruits. Variation of TSS, TA percent and ascorbic acids was observed according to the agro ecological zone.
文摘Acid lime (Citrus aurantifolia) fruits are cultivated in terai, mid hill and high hill districts of Nepal. It is cross-pollinated crop and has a high variation in fruit characters within the species. Fruit character is the main basis of genotype selection. Study of fruit diversity is of utmost importance to select the elite genotypes for breeding and variety development program. Therefore, total of 620 fruit samples were collected and evaluated in the main production season of the year 2010 and 2011, from 62 bearing trees. Samples were collected from three agro-ecological domains, representing terai, mid hill and high hill areas of eastern Nepal. Elite landraces were selected on the basis of scoring fruit characters. High variation in vitamin C was observed between the terai (57.4 mg) and high hill (72.5 mg) samples. Amount of juice was 36.8% in mid hill and 44.1% in terai samples. Higher percent of juice and total soluble solids (TSS) observed in terai samples but tritable acidity (TA) and vitamin C was low as compare to high hills. There was significantly negative correlation found in fruit weight, fruit diameter, juice percent and seed number, whereas significantly positive correlation was in pulp and vitamin C, with altitude range. Total landraces were separated in five major cluster group. Variation in the level of similarity was observed among the cluster groups. The variability distance of fruit characters was highest in cluster group II (–17.45%) and lowest in cluster group V (58.5%). On the basis of scoring number of fruit characters, total four elite genotypes i.e. LD-49 from mid hills, LD-23 and LT-17 from higher hills and LM-44 from terai were selected for conservation, breeding and variety development purpose.
文摘Acid lime (Citrus aurantifolia) is an important commercial fruits crop, cultivated in terai to high hills of Nepal. High variation of acid lime fruits are observed in existing landraces due to crossing within the other citrus species. Determination of genetic variation is important to the plant breeders for development of high yielding variety and hybrids. Therefore, an attempt has been made to study the genetic diversity of 62 acid lime landraces, collected from different altitudinal range in the eastern part of Nepal, using SSR markers. Twelve Simple Sequence Repeat (SSR) primer pairs were used to assess the genetic diversity of acid lime. The average genetic similarity level among the 62 accessions was 0.77, ranging from 0.54 to 1.0 and separated five major cluster groups. Total of 33 alleles were detected by eleven primer pairs and size of alleles ranged from 50 to 225. Average polymorphic information content (PIC) value was 0.50, whereas highest 0.75 and lowest 0.18 was observed in CAT01 and GT03 loci respectively. The results of the study clearly indicated that, SSR markers are highly polymorphic and more informative for the assessment of genetic diversity of acid lime landraces.
