Varietal Difference in Leaf Nitrogen Content and Leaf Area and Their Effects to Ripening Rate During Mature Period of japonica Rice

Employing the pot experiment of the complete random block design with 6 replications,four varieties of japonica rice (Fujisaka 5,Honenwase,Akitakomachi and Taichung 65) were used to study the varietal differences in leaf nitrogen content(LNC) and leaf area during mature period,their relation and effects to the ripening rate.The results showed that(1) thee were varietal differences in LNC at the heading stage and the LNC decrease rate during the matue period,the high LNC at the heading stage was related to the rapid LNC decrease.(2) There were two phases of the leaf area changing process during the mature period,first was the stable,and second was the decreased phase.There was varietal difference in the critical time of phase 1 and phase 2.The hign leaf area in the phase 1 was in relation to the rapid leaf area decrease in the phase 2.It was not found that there was relation between the leaf quality and quantity.(3)It wa unfavorable to the ripening rate for the high leaf area at the heading stage and the rapid decrease of the leaf area during the mature period.(4)It was put forward that the super high yield rice variety should possess the not very high leaf area and high LNC at the heading stage,slow senescence in the leaf area during the mature period.

Effects of Plant Density on Forage Nutritive Value of Whole Plant Corn

In the field experiment, the effects of plant densities (75 000, 112 500 and 150 000 plants ha-1) on forage nutritive value of whole plant corn (WPC) were studied from 1999 to 2001. The results demonstrated that with the increasing of plant density, the forage matter yield per plant corn decreased significantly, while the fresh matter and dry matter per hectare corn increased significantly, and a higher grains yield was gotten at higher plant densities. Forage nutritive quality of whole plant corn was changed as plant density increased, the crude protein (CP), ether extract (EE), crude fiber (CF), nitrogen free extract (NFE) and general energy (GE) yields increased obviously. Increas- ing plant density reasonably with the application of plant growth regulators could improve plant properties, harvest more forage matter, and enhance forage nutritive value of WPC.

Identification of Co-Segregating RAPD Marker Linked to Powdery Mildew Resistance Gene Pm18 in Wheat

The Pm18 gene of wheat confers resistance to the powdery mildew which is one of the mostserious diseases in many regions of the world. In this study, bulked segregant analysis(BSA) was used to develop randomly amplified polymorphic DNA (RAPD) markers linked toPm18 gene. Three hundred and twenty decamer primers were screened and one of them wasidentified as RAPD marker (S411600) linked to Pm18. Using the F2 mapping population fromthe cross Pm18Chancellor, the marker S411600 was shown to co-segregate with the genePm18. This marker can be conveniently used for marker-assisted selection in wheatbreeding programs for the identification or pyramiding of Pm18 with other resistancegenes.

Comparison of Different Dough Rheological Measurement and Path Coefficient Analysis of Bread Quality

Farinograph, extensograph and mixograph are the special instruments used to determinedough rheological characteristics. In this study, twenty-seven wheat cultivars ofdifferent gluten strength were used to study the correlations among each rheologicalparameter determined by above instruments. Multiple linear regression analysis and pathcoefficient analysis were used to study the direct and indirect effects of 11 doughrheological characteristics on bread quality. The results showed significant correlationsamong the principal parameters. There were significantly or extremely significantlypositive correlations among development time (DT), stability time (ST), farinographquality number (FQN) of farinograph, area, maximum resistance (Rmax), viscoelastic ratio(Rmax/E)of extensograph and mixing time (MT), 8-minute-curve-tail (8MCT) of mixograph.These indexes affected bread-making quality either directly or indirectly. Of all theindexes, ST, maximum Rmax, MT and FQN were the most important ones.

Effect of Adjuvants on Activity and Persistence of Soil-applied Herbicide Anwei

The paper researched effect of adjuvants on activity and persistence of soil-applied herbicide Anwei.There were five adjuvants (fertility, Q7, oil, anionic and nonionic surfactants) in the experiment. We explored the influence of adjuvants on activity and persistence of Anwei by field trial. The results indicated that Q7, oil, anionic surfactant can increased activity of Anwei, and can lengthened its persistence for about five days.

Identification of a Long Rice Spikelet Mutant

A spontaneously occurring rice (Oryza sativa L.) mutant, characterized by homeotic conversionin glumes and stamens, was found in the progeny of a cross. The mutant showed long glumes andglumaceous lodicules and morphological transformation of stamens into pistils. Mutant floretsconsisted of 1 to 3 completely developed pistils, some pistilloid stamens with filaments, buttipped by bulged tissue and 0 to 3 stigmas. It seems that the mutant phenotype of the homeoticconversions in glumes and stamens is similar to that of the B loss-of-function mutants inArabidopsis and Antirrhinum. The mutant is controlled by a single recessive gene as asegregation ratio of 3:1 (wild type to mutant plants) was observed in the F2 generation.

Cloning of a novel phosphateserine aminotransferase gene from a Triticum aestivum-Elytrigia elongatum alien substitution line with resistance to powdery mildew

Shannong 551, a T. aestivum-E. elongatum alien substitution line with resistance to powdery mildew, was in- oculated with pathogenic spores of powdery mildew. The leaf samples were prepared 48 h after inoculation for scanning electron microscopy. The result showed that germination of spores and growth of young mycelia on leaves of Shannong 551 were suppressed at the early stage of infection. At the same time, RNAs were prepared from the leaves for the cloning of WRP1 and RPW2 by cDNA RDA and RACE tech- nology. BLAST analysis of the sequences indicated that both WRP1 and RPW2 were novel genes. WRP1 contains no com- plete ORF. RPW2 contains the conserved structure domain of aminotransferase, and its DNA sequence shares high homol- ogy with genes of phosphateserine aminotransferase in many organisms. Therefore, it is speculated as a novel phosphate- serine aminotransferase gene. The results of Northern blot suggested that expression of RPW2 occurred at the early stage of infection by powdery mildew. Southern blot using the probe of RPW2, in which there was strong hybridizing sig- nals in both genome of Shannong 551 and E. elongatum, but not in those of Jinan 13 and Lumai No.5, indicated that RPW2 derived from the genome of E. elongatum.