Objective:To study the effect of high glucose environment on oxidative stress injury of human retinal pigment epithelium (RPE) as well as pithelial-mesenchymal transition.Methods:Human PRE cells were cultivated and di...Objective:To study the effect of high glucose environment on oxidative stress injury of human retinal pigment epithelium (RPE) as well as pithelial-mesenchymal transition.Methods:Human PRE cells were cultivated and divided into low glucose control group, hyperosmosis control group and high glucose intervention group that were treated with DMEM medium with glucose concentration 5.5 mmol/L, DMEM medium with osmotic concentration 60.0 mmol/L and glucose concentration 5.5 mmol/L as well as the DMEM medium with glucose concentration 60.0 mmol/L respectively, and after 24 h, the levels of oxidative stress molecules, cell apoptosis molecules and mesenchymal cell marker molecules in cells were determined. Results:ROS, MDA, 3-NT, Nrf2, ARE, Caspase-3, Bax, JNK, c-JUN,α-SMA, Vimentin and N-cadherin levels in high glucose intervention group were significantly higher than those of low glucose control group and hyperosmosis control group while GST and HO-1 levels were significantly lower than those of low glucose control group and hyperosmosis control group;ROS, MDA, 3-NT, Nrf2, ARE, Caspase-3, Bax, JNK, c-JUN,α-SMA, Vimentin, N-cadherin, GST and HO-1 levels were not significantly different between low glucose control group and hyperosmosis control group.Conclusion:High glucose environment can enhance the oxidative stress response of RPE cells to start the cell apoptosis and epithelial-mesenchymal transition process.展开更多
文摘Objective:To study the effect of high glucose environment on oxidative stress injury of human retinal pigment epithelium (RPE) as well as pithelial-mesenchymal transition.Methods:Human PRE cells were cultivated and divided into low glucose control group, hyperosmosis control group and high glucose intervention group that were treated with DMEM medium with glucose concentration 5.5 mmol/L, DMEM medium with osmotic concentration 60.0 mmol/L and glucose concentration 5.5 mmol/L as well as the DMEM medium with glucose concentration 60.0 mmol/L respectively, and after 24 h, the levels of oxidative stress molecules, cell apoptosis molecules and mesenchymal cell marker molecules in cells were determined. Results:ROS, MDA, 3-NT, Nrf2, ARE, Caspase-3, Bax, JNK, c-JUN,α-SMA, Vimentin and N-cadherin levels in high glucose intervention group were significantly higher than those of low glucose control group and hyperosmosis control group while GST and HO-1 levels were significantly lower than those of low glucose control group and hyperosmosis control group;ROS, MDA, 3-NT, Nrf2, ARE, Caspase-3, Bax, JNK, c-JUN,α-SMA, Vimentin, N-cadherin, GST and HO-1 levels were not significantly different between low glucose control group and hyperosmosis control group.Conclusion:High glucose environment can enhance the oxidative stress response of RPE cells to start the cell apoptosis and epithelial-mesenchymal transition process.
