Response of wild Avena species to fungal infection of grain

Sixty-six accessions belonging to Avena species(A. atlantica, A. canariensis, A. clauda, A.damascena, A. hirtula, A. longiglumis, A. wiestii, A. agadiriana, A. barbata, A. vaviloviana, A.insularis, A. magna, A. murphyi, A.fatua, A. ludoviciana, A. occidentalis, A. sterilis, A. sativa, and A. byzantina) obtained from the N. I. Vavilov Institute of Plant Genetic Resources(VIR) were tested for the infection of grain by Alternaria and Cladosporium at the genus level and by a group of trichothecene-producing Fusarium fungi. Real-time PCR was performed to quantify fungal abundance and ELISA was performed for mycotoxin analysis in grain. The average amounts of all tested fungi in tetraploid Avena species were higher than those in diploid and hexaploid species. Low amounts of deoxynivalenol(DON) were detected in seven hexaploid genotypes(A. sterilis, A. byzantina, A. sativa, and A. fatua) and one diploid genotype(A.lwiestii). The relationship between some morphological traits(1000-grain weight, percent of husk, trichome density, and plant height) of Avena species and grain infection by fungi and mycotoxins was investigated. Alternaria and Cladosporium fungi could not penetrate the husk. A highly negative correlation of the amounts of their DNA with the proportions of husk in grain as well as with trichome density was found. In contrast, a significant positive correlation between the amount of Fusarium DNA and these traits was found. A strong negative correlation bctween plant height and DNA of the analyzed fungi was found. The oats least infected by Alternaria, Cladosporium, and Fusarium fungi and containing low amounts of DON were accessions of the hexaploids A. byzantina, A.fatua, A. sativa, A. sterilis,and the diploid A. lwiestii.

Notes for genera:basal clades of Fungi(including Aphelidiomycota,Basidiobolomycota,Blastocladiomycota,Calcarisporiellomycota,Caulochytriomycota,Chytridiomycota,Entomophthoromycota,Glomeromycota,Kickxellomycota,Monoblepharomycota,Mortierellomycota,Mucoromycota,Neocallimastigomycota,Olpidiomycota,Rozellomycota and Zoopagomycota)

Compared to the higher fungi(Dikarya),taxonomic and evolutionary studies on the basal clades of fungi are fewer in number.Thus,the generic boundaries and higher ranks in the basal clades of fungi are poorly known.Recent DNA based taxonomic studies have provided reliable and accurate information.It is therefore necessary to compile all available information since basal clades genera lack updated checklists or outlines.Recently,Tedersoo et al.(MycoKeys 13:1–20,2016)accepted Aphelidiomycota and Rozellomycota in Fungal clade.Thus,we regard both these phyla as members in Kingdom Fungi.We accept 16 phyla in basal clades viz.Aphelidiomycota,Basidiobolomycota,Blastocladiomycota,Calcarisporiellomycota,Caulochytriomycota,Chytridiomycota,Entomophthoromycota,Glomeromycota,Kickxellomy-cota,Monoblepharomycota,Mortierellomycota,Mucoromycota,Neocallimastigomycota,Olpidiomycota,Rozellomycota and Zoopagomycota.Thus,611 genera in 153 families,43 orders and 18 classes are provided with details of classification,synonyms,life modes,distribution,recent literature and genomic data.Moreover,Catenariaceae Couch is proposed to be conserved,Cladochytriales Mozl.-Standr.is emended and the family Nephridiophagaceae is introduced.

FungalTraits:a user-friendly traits database of fungi and fungus-like stramenopiles

The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental studies.Over the past decades,rapid development and affordability of molecular tools have tremendously improved insights of the fungal diversity in all ecosystems and habitats.Yet,in spite of the progress of molecular methods,knowledge about functional properties of the fungal taxa is vague and interpretation of environmental studies in an ecologically meaningful manner remains challenging.In order to facilitate functional assignments and ecological interpretation of environmental studies we introduce a user friendly traits and character database FungalTraits operating at genus and species hypothesis levels.Combining the information from previous efforts such as FUNGuild and FunFun together with involvement of expert knowledge,we reannotated 10,210 and 151 fungal and Stramenopila genera,respectively.This resulted in a stand-alone spreadsheet dataset covering 17 lifestyle related traits of fungal and Stramenopila genera,designed for rapid functional assignments of environmental stud-ies.In order to assign the trait states to fungal species hypotheses,the scientific community of experts manually categorised and assigned available trait information to 697,413 fungal ITS sequences.On the basis of those sequences we were able to summarise trait and host information into 92,623 fungal species hypotheses at 1%dissimilarity threshold.

Correction to:FungalTraits:a user friendly traits database of fungi and fungus-like stramenopiles

Correction to:Fungal Diversity(2020)105:116 https://doi.org/10.1007/s13225-020-00466-2 There were errors in the name of author LászlóG.Nagy and in affiliation no.31 in the original publication.The original article has been corrected.