Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive dysfunction. Currently there is no effective treatment. Given the depth of current stem cell research,...Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive dysfunction. Currently there is no effective treatment. Given the depth of current stem cell research, researchers have attempted to compensate for neuronal loss in the brains of patients with Alzheimer's disease using stem cell technology. This article will review the pathogenesis of AD, the latest advances in stem cell therapy, and the challenges and prospects of its development.展开更多
Objective:To construct a recombinant lentiviral vector that co-express ZsGreen and BDNF and establish an Adipose derived stem cell (ADSC) line with stable BDNF expression down- regulation.Methods: BDNF were designed a...Objective:To construct a recombinant lentiviral vector that co-express ZsGreen and BDNF and establish an Adipose derived stem cell (ADSC) line with stable BDNF expression down- regulation.Methods: BDNF were designed and inserted into the lentiviral vector p HBLV-CMVIE-Zs Green-Puro. After identification by DNA sequencing, the lentiviral vectors carrying BDNF were packaged in 293 cells. The transfection efficiency was observed under fluorescence microscope;the lentiviral particles were collected to infect mouse ADSCs, and BDNF level were assessed using real-time PCR and Western blotting.Results: DNA sequencing demonstrated successful construction of the BDNF lentivirus vectors. Real- time PCR and Western blotting showed a high level BDNF mRNA and protein.Conclusion: We have successfully established an ADSC model with stable BDNF expression which establish the basis for the potential application of ADSCs-BDNF in the treatment of AD.展开更多
基金National Natural Science Foundation of China (81660224,31760310)
文摘Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive dysfunction. Currently there is no effective treatment. Given the depth of current stem cell research, researchers have attempted to compensate for neuronal loss in the brains of patients with Alzheimer's disease using stem cell technology. This article will review the pathogenesis of AD, the latest advances in stem cell therapy, and the challenges and prospects of its development.
基金The National Natural Science Foundation of China.Project No:(81660224,81100246)Hainan Provincial Key Research and Development Project.Project No:(ZDYF2017120,ZDYF2016110)+2 种基金National College’s Students’Innovation and Entrepreneurship Training Program Project.Project No:(201711810010,HYCX2016010,HYCX2018032)Hainan Provincial Health and Family Planning Industry Research Project.Project No:(18A200115)Haikou People’s Hospital Project.Project No:(2018-SNP-01-003).
文摘Objective:To construct a recombinant lentiviral vector that co-express ZsGreen and BDNF and establish an Adipose derived stem cell (ADSC) line with stable BDNF expression down- regulation.Methods: BDNF were designed and inserted into the lentiviral vector p HBLV-CMVIE-Zs Green-Puro. After identification by DNA sequencing, the lentiviral vectors carrying BDNF were packaged in 293 cells. The transfection efficiency was observed under fluorescence microscope;the lentiviral particles were collected to infect mouse ADSCs, and BDNF level were assessed using real-time PCR and Western blotting.Results: DNA sequencing demonstrated successful construction of the BDNF lentivirus vectors. Real- time PCR and Western blotting showed a high level BDNF mRNA and protein.Conclusion: We have successfully established an ADSC model with stable BDNF expression which establish the basis for the potential application of ADSCs-BDNF in the treatment of AD.
