[ Objective ] This study was to investigate the creditability of applying loop-mediated isothermal amplification method （LAMP） to detect Staphylococcus aureus in dairy products. [ Methods] The primers for heat resis...[ Objective ] This study was to investigate the creditability of applying loop-mediated isothermal amplification method （LAMP） to detect Staphylococcus aureus in dairy products. [ Methods] The primers for heat resistant nuclease gene （nuc） of Staphylococcus aureus were designed for establishing the LAMP method for rapidly detecting Staphylococcus aureus. [ Results ] The results of LAMP detection on Staphylococcus aureus in various dairy products were completely identical with that by bacterial isolation test; meanwhile it has a high specificity and a 10-fold sensitivity over the hemi-nested PCR. [ Conclusion] LAMP can be used for the detection of Staphylococcus aureus in dairy products.展开更多
文摘[ Objective ] This study was to investigate the creditability of applying loop-mediated isothermal amplification method （LAMP） to detect Staphylococcus aureus in dairy products. [ Methods] The primers for heat resistant nuclease gene （nuc） of Staphylococcus aureus were designed for establishing the LAMP method for rapidly detecting Staphylococcus aureus. [ Results ] The results of LAMP detection on Staphylococcus aureus in various dairy products were completely identical with that by bacterial isolation test; meanwhile it has a high specificity and a 10-fold sensitivity over the hemi-nested PCR. [ Conclusion] LAMP can be used for the detection of Staphylococcus aureus in dairy products.
