Study on the correlation between TCM syndrome differentiation and immune inflammation index of Sjogren's syndrome

Objective:To observe the relationship between the distribution of TCM Syndromes of primary Sjogren's syndrome(pSS)and the changes of erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),immunoglobulin IgA,IgG and IgM.Methods:157 diagnosed PSS patients were divided into dampness heat obstructing collaterals syndrome,dryness evil invading lung syndrome,Qi blood stasis syndrome,liver kidney yin deficiency syndrome and Qi Yin deficiency syndrome according to TCM syndrome differentiation standard.The age,course of disease,general health score in international universal quality of life scale,self rating Anxiety scale(SAS),self rating Depression Scale(SDS)and erythrocyte sedimentation rate were observed and detected(ESR,C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),immunoglobulin IgA,IgG and IgM and other immune inflammatory indexes were detected.The relationship between different TCM Syndromes of PSS and molecular level changes of immune inflammatory indexes was analyzed and compared,as well as the impact on quality of life and mental emotion.Results:The overall health score of patients with Qi Yin deficiency syndrome was significantly lower than that of patients with dampness heat obstructing collaterals and dampness evil invading lung syndrome(P<0.01),while the SAS and SDS scores of patients with Qi Yin deficiency syndrome were significantly higher than those of patients with dampness heat invading lung syndrome and dampness heat obstructing collaterals syndrome(P<0.05,P<0.01);ESR,CRP,RF,TNF-α,IL-6,IgA,IgG and IgM related laboratory indexes of patients with dampness heat obstructing collaterals syndrome were significantly higher than those of patients with dry evil invading lung syndrome,liver kidney yin deficiency syndrome and liver kidney yin deficiency syndrome Qi Yin deficiency syndrome and Qi blood stasis syndrome(P<0.05,P<0.01).Correlation test found that the above five syndrome types were positively correlated with hs-CRP,IL-6,TNF-ɑindicators(P<0.05).Conclusion:The overall health,SAS,SDS and related hs-CRP,IL-6,TNF-ɑand some others indexes of SS patients are associated with TCM syndromes.

System evaluation and Meta analysis on clinical effi cacy of heat-sensitive moxibustion in treatment of cervical spondylotic radiculopathy

Objective To systematically evaluate the efficacy of heat-sensitive moxibustion in treatment of cervical spondylotic radiculopathy（CSR）. Methods Chinese National Knowledge Infrastructure（CNKI）, China Biology Medical（CBM） database, Chinese Science and Technology Periodical Database（VIP）, Wan Fang Data, Pubmed and Cochrane Library were retrieved to search the randomized controlled trials（RCT） on heat-sensitive moxibustion in treatment of CSR, and Meta analysis was conducted by applying Rev Man 5.3 software. Results Eventually, 10 papers were included in this study, including 1008 subjects. Meta analysis result showed： the total effective rate of treatment group was higher than that of control group, and the difference was statistically significant [RR=1.13, 95%CI（1.06, 1.21）, Z=3.54, P=0.000 4]; the cure rate of treatment group was higher than that of control group, and the difference was statistically significant [RR=1.80, 95%CI（1.52, 2.13）, Z=6.82, P〈0.000 01]; the improvement of short-form Mc Gill pain questionnaire（SF-MPQ） of treatment group was superior to that of control group, and the difference was statistically significant [MD=–4.44, 95%CI（–6.38,-2.50）, Z=4.49, P〈0.000 01]; visual analogue scale（VAS） of treatment group was lower than that of control group, and the difference was statistically significant [MD=-0.36, 95%CI（-0.50, –0.23）, Z=5.42, P〈0.00001]; and the improvement of interleukin-6（IL-6） of treatment group was superior to that of control group, and the difference was statistically significant [MD=–7.32, 95%CI（-11.49, –3.14）, Z=3.44, P=0.000 6]. Conclusion It is indicated from the Meta analysis result that the clinical efficacy of heat-sensitive moxibustion or acupuncture combined with heat-sensitive moxibustion in treatment of CSR is superior to that of simple acupuncture or traditional suspended moxibustion, providing a new therapeutic method for treatment of CSR. However, the abovementioned conclusion still needs to be confirmed through randomized blind controlled trials with high quality and large sample size since the sample size of included studies was small, and the quality was low.

Regulating effect of moxibustion on MCP-1 and NF-κB in the colonic mucosa tissue of rats with Crohn's disease

Objective To observe the effect of moxibustion on the expression levels of MCP-1 and NF-κB proteins in the colonic mucosa tissue of rats with Crohn＇s disease（CD）. Methods Morris method, which is internationally recognized, was adopted to establish CD rat models. The rats were randomly divided into blank control group, CD model group and moxa stick moxibustion group. In the moxa stick moxibustion group, the cigarette-like moxa sticks were used for suspended moxibustion at the locations 2 cm from Píshū（脾俞 BL 20） and Zhōngwǎn（中脘 CV 12）. The treatment was conducted once a day, lasted for 20 min at each time, and 7 times were needed; one acupoint was used for each time, and the two acupoints were used alternately. Under light microscope, HE staining was used to observe the pathological changes of colon, and immunohistochemical method was adopted to observe the expression levels of MCP-1, NF-κB p 65 and NF-κB p 50 proteins in colonic mucosa tissue of rats. Results Compared with the blank control group, in CD model group, mucosa and glands defect or disappearance, villus damage, submucosal congestion and edema, and elcosis were found; the average optical density of the expression of MCP-1, NF-κB p 50 and NF-κB p 65 proteins in the colonic mucosa tissue of rats significantly increased（P〈0.01）. Compared with the CD model group, in the moxa stick moxibustion group, the inflammatory response of colonic mucosa of rats improved significantly, mainly manifesting as relieved submucosal edema, a small amount of inflammatory cells infiltration, and regular intestinal glands distribution; the average optical density of the expression of MCP-1, NF-κB p 50 and NF-κB p 65 proteins in the colonic mucosa tissue of rats significantly decreased（P〈0.05）. Conclusions Moxibustion can down-regulate the expression levels of MCP-1 and NF-κB proteins in the colonic mucosa of CD rats.