Trypanosomosis is major drawback to profitable livestock production in sub-Sahara African, including Nigeria. Knowledge of the cytokines production in the phase of natural infection may help to better diagnose, treat ...Trypanosomosis is major drawback to profitable livestock production in sub-Sahara African, including Nigeria. Knowledge of the cytokines production in the phase of natural infection may help to better diagnose, treat and prevent bovine trypanosomosis. The purpose of the this study was to determine the levels of interferon-gamma (IFN-γ), interleukin-10 (IL-10) and cardiac troponin–I (cTnI) in the sera of cattle naturally infected with T. brucei, T. congolense and T. vivax and correlate these levels with parasitaemia and PCV of the infected animals. Five milliliter of blood samples were collected via the jugular vein from 411 randomly selected cattle into EDTA and non-citrated bottle. PCV was determined manually using HCT. Trypansomes were detected and characterized by microscopy and PCR, respectively. Serum levels of IFN-γ, IL-10 and cTnI were determined using commercial ELISA kit. Data were summarized using descriptive statistic and significance of differences determined by ANOVA. Of the 62 samples positive for trypanosomes by microscopy, 50 samples were confirmed to species level by PCR. The sera levels of IFN-γ, IL-10 and cTnI of infected cattle were higher than non-infected cattle. The differences were not significant (p γ, IL-10 and cTnI in cattle with natural trypanosomosis. Further investigation is required to understand the specific effect of trypanosomes on myocardiac integrity and interaction between the two cytokines in natural trypanosomosis in cattle.展开更多
Genomic imprinting is an epigenetic process that regulates gene expression in the mammalian genome. Although there are specific imprinting differences between the mammalian species, cattle present unique opportunity f...Genomic imprinting is an epigenetic process that regulates gene expression in the mammalian genome. Although there are specific imprinting differences between the mammalian species, cattle present unique opportunity for characterizing imprinted genes because of its sub-species classification. Five putative imprinted genes (TSSC4, CDKN1C, KCNQ1, PHLDA2 and NAP1L4) on bovine chromosome 29 (Bta 29) which had been characterized to have promoter CGI were quantitatively assayed for their relative expression across eight tissues (muscle, brain, liver, kidney, spinal cord, heart, lymph and skin) sampled in Angus cattle. The differential abundance of these genes in muscle and skin tissues of Angus, White Fulani and N’Dama cattle breeds was comparatively analyzed. These three breeds are representative of the Bos taurus and Bos indicus cattle sub-species while the two tissues are selected based on their strategic economic importance in cattle production. All the genes, except TSSC4, were relatively expressed across all the tissues. It was observed that Angus had the highest differential abundance in muscle tissues for TSSC4, PHLDA2 and NAP1L4 while N’Dama and White Fulani were the most abundant for KCNQ1 and NAP1L4 in skin tissues. The study identified marked differences in the expression profiles of the genes in both muscle and skin tissues of the three breeds that were characteristics of their genetics, environment and nutrition.展开更多
文摘Trypanosomosis is major drawback to profitable livestock production in sub-Sahara African, including Nigeria. Knowledge of the cytokines production in the phase of natural infection may help to better diagnose, treat and prevent bovine trypanosomosis. The purpose of the this study was to determine the levels of interferon-gamma (IFN-γ), interleukin-10 (IL-10) and cardiac troponin–I (cTnI) in the sera of cattle naturally infected with T. brucei, T. congolense and T. vivax and correlate these levels with parasitaemia and PCV of the infected animals. Five milliliter of blood samples were collected via the jugular vein from 411 randomly selected cattle into EDTA and non-citrated bottle. PCV was determined manually using HCT. Trypansomes were detected and characterized by microscopy and PCR, respectively. Serum levels of IFN-γ, IL-10 and cTnI were determined using commercial ELISA kit. Data were summarized using descriptive statistic and significance of differences determined by ANOVA. Of the 62 samples positive for trypanosomes by microscopy, 50 samples were confirmed to species level by PCR. The sera levels of IFN-γ, IL-10 and cTnI of infected cattle were higher than non-infected cattle. The differences were not significant (p γ, IL-10 and cTnI in cattle with natural trypanosomosis. Further investigation is required to understand the specific effect of trypanosomes on myocardiac integrity and interaction between the two cytokines in natural trypanosomosis in cattle.
文摘Genomic imprinting is an epigenetic process that regulates gene expression in the mammalian genome. Although there are specific imprinting differences between the mammalian species, cattle present unique opportunity for characterizing imprinted genes because of its sub-species classification. Five putative imprinted genes (TSSC4, CDKN1C, KCNQ1, PHLDA2 and NAP1L4) on bovine chromosome 29 (Bta 29) which had been characterized to have promoter CGI were quantitatively assayed for their relative expression across eight tissues (muscle, brain, liver, kidney, spinal cord, heart, lymph and skin) sampled in Angus cattle. The differential abundance of these genes in muscle and skin tissues of Angus, White Fulani and N’Dama cattle breeds was comparatively analyzed. These three breeds are representative of the Bos taurus and Bos indicus cattle sub-species while the two tissues are selected based on their strategic economic importance in cattle production. All the genes, except TSSC4, were relatively expressed across all the tissues. It was observed that Angus had the highest differential abundance in muscle tissues for TSSC4, PHLDA2 and NAP1L4 while N’Dama and White Fulani were the most abundant for KCNQ1 and NAP1L4 in skin tissues. The study identified marked differences in the expression profiles of the genes in both muscle and skin tissues of the three breeds that were characteristics of their genetics, environment and nutrition.
