Experiments were performed to evaluate the efficiency of inhibition of HSP70 gene expressionby antisense oligonucleotides complementary to the mRNA of HSP70 and to test the effects ofinhibition of HSP70 gene expressio...Experiments were performed to evaluate the efficiency of inhibition of HSP70 gene expressionby antisense oligonucleotides complementary to the mRNA of HSP70 and to test the effects ofinhibition of HSP70 gene expression on subsequent embryonic sensitivity to heat shock. Theresults showed that transfection of pre-implantation embryos at 4-cell stage with 5 Mantisense oligo had no effect on in vitro blastocyst development. However, transfection with10 to 40 M antisense oligo had reduced in vitro blastocyst development to 15, 10% and 0; Forthe embryos which exposed to 40 M As arrested at the 16-cell stage, there was no blastocystformation within the heat shock groups. In contrast, transfection had no effect on embryonicsensitivity to heat shock, above 25% of embryos developed to blastocyst stage in controlgroups.展开更多
Arpijaza,Lekezhi and Zhangmu cattle discovered in cooled region of Tibet are Bos populations which have close blood relationship with zebu. Besides studying their distribution, nature environment and ecological condit...Arpijaza,Lekezhi and Zhangmu cattle discovered in cooled region of Tibet are Bos populations which have close blood relationship with zebu. Besides studying their distribution, nature environment and ecological conditions, the multivariate statistics analysis of three sides (i. e. , body measurement, morphology and ecology characters) were carried out on three zebu populations from cooled region of Tibet and other three reference populations. The results indicated that there were various ecology types within Tibet zebu populations. Fore two character values were selected as the principal components (occupied 88.15% of total information amount). The cluster analysis according to two principal component values of each sample displayed that three zebu populations from Tibet and two zebu populations from Yunnan emerged in clustering figure alternately, Lhasa yellow cattle was independent from the five zebu populations. The study has proved that there were zebu populations in Tibet cooled region and there was a possibility of blood relationship between the zebu populations from Tibet and Yunnan.展开更多
基金supported financially by National(30270957)Shandong(Y2003D03)Natural Science Foundation of China
文摘Experiments were performed to evaluate the efficiency of inhibition of HSP70 gene expressionby antisense oligonucleotides complementary to the mRNA of HSP70 and to test the effects ofinhibition of HSP70 gene expression on subsequent embryonic sensitivity to heat shock. Theresults showed that transfection of pre-implantation embryos at 4-cell stage with 5 Mantisense oligo had no effect on in vitro blastocyst development. However, transfection with10 to 40 M antisense oligo had reduced in vitro blastocyst development to 15, 10% and 0; Forthe embryos which exposed to 40 M As arrested at the 16-cell stage, there was no blastocystformation within the heat shock groups. In contrast, transfection had no effect on embryonicsensitivity to heat shock, above 25% of embryos developed to blastocyst stage in controlgroups.
基金This study was supported by the National Natural Science Foundation of China(39670530)the Key Program of Agricultural Department of China.
文摘Arpijaza,Lekezhi and Zhangmu cattle discovered in cooled region of Tibet are Bos populations which have close blood relationship with zebu. Besides studying their distribution, nature environment and ecological conditions, the multivariate statistics analysis of three sides (i. e. , body measurement, morphology and ecology characters) were carried out on three zebu populations from cooled region of Tibet and other three reference populations. The results indicated that there were various ecology types within Tibet zebu populations. Fore two character values were selected as the principal components (occupied 88.15% of total information amount). The cluster analysis according to two principal component values of each sample displayed that three zebu populations from Tibet and two zebu populations from Yunnan emerged in clustering figure alternately, Lhasa yellow cattle was independent from the five zebu populations. The study has proved that there were zebu populations in Tibet cooled region and there was a possibility of blood relationship between the zebu populations from Tibet and Yunnan.
