基于高脂血症斑马鱼模型和网络药理学探讨黄鳝肽抗高脂血症的作用机制

采用高血脂症斑马鱼模型和网络药理学的方法探讨黄鳝肽抗高脂血症的物质基础及作用机制。构建高血脂斑马鱼模型及测试甘油三酯染色信号强度、胆固醇荧光强度指标,分析黄鳝肽对高血脂症的改善作用;利用BIOPEP-UWM、SwissTargetPrediction、GeneCards、STRING等数据库筛选黄鳝肽及疾病靶点,使用DAVID软件进行靶点GO功能富集分析和KEGG通路富集分析,并构建黄鳝肽-潜在靶点-信号通路网络和蛋白互作(PPI)网络。结果显示,质量浓度为62.5、125.0μg/mL的黄鳝肽可显著降低高脂血症斑马鱼血管中的甘油三酯染色信号强度(p<0.01)、胆固醇荧光强度(p<0.001)。网络药理学结果显示黄鳝肽中有35个潜在活性肽段序列,通过蛋白互作分析得到21个核心靶点,GO和KEGG富集分析表明黄鳝肽主要涉及脂质代谢过程的调节等生物过程,通过调节炎症、胰岛素抵抗及脂质和动脉粥样硬化等通路发挥抗高脂血症的作用。研究结果初步证实了黄鳝肽具有抗高血脂症功效,揭示了黄鳝肽降血脂生物过程中多活性肽段、多靶点、多通路的作用特点,为进一步深入研究黄鳝肽物质基础及抗高脂血症作用与应用提供理论依据及参考。

Recovery approaches in mental health: A qualitative evaluation of the Whole Life Therapy programme for persons with schizophrenia

The recovery approach within mental health services has in recent years been influential in promoting more active participation from service users concerning their treatment and progress, within a move towards models of interventions based on social models and ideas of service user empowerment. Although mental health recovery models are often heralded as ideological goals, comparatively little has been documented about the means of achieving these. This article sets out the nature and content of the Whole Life Programme, used within the Hertfordshire NHS Partnership Foundation Trust, and the results of qualitative research into the programme that set out to analyse the impact of its delivery from the perspectives of service users. The research examined the experiences and views of participants receiving treatment several months after the completion of the programme, and also of those who withdrew prematurely, in order to learn from these experiences, adding to our understanding of how one recovery based approach, the Whole Life Manual, can be applied in practice.

Erratum to “Recovery Approaches in Mental Health: A Qualitative Evaluation of the Whole Life Therapy Programme for Persons with Schizophrenia” [Health 5 (2013) 582-587]

The original online version of this article (Littlechild, B., Smith, A. H., Meredith-Windle, G., Gale, T., Lloyd, M. and Hawley, C. (2013) Recovery Approaches in Mental Health: A Qualitative Evaluation of the Whole Life Therapy Programme for Persons with Schizophrenia. Health, 5, 582-587. http://dx.doi.org/10.4236/health.2013.53A077) was published mistakenly with a co-author (who also developed the Whole Life Programme) missing. To reflect the contribution and responsibility of the co-author as well as the affiliations of the authors at the time of the study, we revised authorship and author affiliations of the article.

基于斑马鱼模型的黄鳝肽减肥作用研究

目的:以模式生物斑马鱼为实验对象,研究黄鳝肽的减肥作用。方法:以白藜芦醇为阳性对照药物,分别给予不同浓度的黄鳝肽,分析斑马鱼卵黄囊脂肪荧光强度,评价黄鳝肽促进脂肪分解功效;采用高脂饲料饲喂斑马鱼建立脂肪吸收模型,以奥利司他为阳性对照药物,分别给予不同浓度的黄鳝肽,分析斑马鱼肠道和尾部的血管脂肪染色强度,评价黄鳝肽抑制脂肪吸收功效。结果:与正常对照组相比,黄鳝肽中剂量组(62.5μg·mL^(-1))、高剂量组(125.0μg·m L^(-1))卵黄囊脂肪荧光信号强度明显降低(P<0.01);与模型对照组相比,黄鳝肽高剂量组(125.0μg·mL^(-1))肠道和尾部血管脂肪染色信号强度明显降低(P<0.05)。结论:黄鳝肽具有抑制脂肪吸收、促进脂肪分解的功效,可作为预防肥胖的功效原料应用于功能食品、保健食品的开发。

复合坚果蔓越莓蛋白棒的饱腹感评价

采用视觉模拟评分法(Visual Analog Scales,VAS),以市售花生巧克力棒为对标样品、白馒头为参比样品,对复合坚果蔓越莓蛋白棒的饱腹感进行评价。结果表明,复合坚果蔓越莓蛋白棒的饱腹感指数为273.63±52.40,显著高于花生巧克力棒的饱腹感指数98.40±20.84(P<0.01)。复合坚果蔓越莓蛋白棒在240 min时的饱腹感数值高于空腹(0 min)饱腹感水平,表明空腹食用300 kcal的复合坚果蔓越莓蛋白棒,可持续4 h的饱腹感。