Objective:To analyze the effect of Cadmium(Cd)on the metabolism of reduced glutathione(GSH)in roots and leaves of Glycine max L.Methods:A capillary electrophoresis methodology was optimized to determinate simultaneous...Objective:To analyze the effect of Cadmium(Cd)on the metabolism of reduced glutathione(GSH)in roots and leaves of Glycine max L.Methods:A capillary electrophoresis methodology was optimized to determinate simultaneously GSH and reduced and oxidized GSH in a precise and accurate way.The functional role of the genes involved in GSH cellular metabolism(γ-glutamylcysteine synthetase),GSH synthetase and glutathione reductase(GR)was evaluated.Finally,the activities of antioxidant enzymes as GR and superoxide dismutases were determinate.Results:The studies ofγ-glutamylcysteine synthetase and GSH synthetase gene expression showed an increase and GR showed a decrease in Cd-treated plants.GR and superoxide dismutases activities increased at 24 h and 6 h respectively in roots under Cd exposure.GSH content was higher and showed a significant increase in roots and leaves at 6 h and 24 h of treatment.Conclusions:The results of the present study showed a better understanding in signaling pathway by alterations in antioxidant mechanisms by Cd in soybean seedlings.展开更多
基金Supported by National Scientific and Technical Research Council(CONICET)the National Agency of Scientific and Technologic Promotion(FONCYT)(PICT-BID)and National University of San Luis(Argentina)PROICO N°2/1112,PROICO N°2/0403 and PROICO N°2/1512.
文摘Objective:To analyze the effect of Cadmium(Cd)on the metabolism of reduced glutathione(GSH)in roots and leaves of Glycine max L.Methods:A capillary electrophoresis methodology was optimized to determinate simultaneously GSH and reduced and oxidized GSH in a precise and accurate way.The functional role of the genes involved in GSH cellular metabolism(γ-glutamylcysteine synthetase),GSH synthetase and glutathione reductase(GR)was evaluated.Finally,the activities of antioxidant enzymes as GR and superoxide dismutases were determinate.Results:The studies ofγ-glutamylcysteine synthetase and GSH synthetase gene expression showed an increase and GR showed a decrease in Cd-treated plants.GR and superoxide dismutases activities increased at 24 h and 6 h respectively in roots under Cd exposure.GSH content was higher and showed a significant increase in roots and leaves at 6 h and 24 h of treatment.Conclusions:The results of the present study showed a better understanding in signaling pathway by alterations in antioxidant mechanisms by Cd in soybean seedlings.
