Biofilm-related biomaterial infections are notoriously challenging to treat and can lead to chronic infection and persisting inflammation.To date,a large body of research can be reviewed for coatings which potentially...Biofilm-related biomaterial infections are notoriously challenging to treat and can lead to chronic infection and persisting inflammation.To date,a large body of research can be reviewed for coatings which potentially prevent bacterial infection while promoting implant integration.Yet only a very small number has been translated from bench to bedside.This study provides an in-depth analysis of the stability,antibacterial mechanism,and biocompatibility of medical grade polycaprolactone(mPCL),coated with human serum albumin(HSA),the most abundant protein in blood plasma,and tannic acid(TA),a natural polyphenol with antibacterial properties.Molecular docking studies demonstrated that HSA and TA interact mainly through hydrogen-bonding,ionic and hydrophobic interactions,leading to smooth and regular assemblies.In vitro bacteria adhesion testing showed that coated scaffolds maintained their antimicrobial properties over 3 days by significantly reducing S.aureus colonization and biofilm formation.Notably,amplitude modulation-frequency modulation(AMFM)based viscoelasticity mapping and transmission electron microscopy(TEM)data suggested that HSA/TA-coatings cause morphological and mechanical changes on the outer cell membrane of s.aureus leading to membrane disruption and cell death while proving non-toxic to human primary cells.These results support this antibiotic-free approach as an effective and biocompatible strategy to prevent biofilm-related biomaterial infections.展开更多
基金supported by the ARC Industrial Transformation Training Centre for Multiscale 3D Imaging,Modelling,and Manufacturing[IC 180100008],NHMRC 2008018-Transformation of the implant paradigm in breast rehabilitation Grantthe Max Planck Queensland Centre.
文摘Biofilm-related biomaterial infections are notoriously challenging to treat and can lead to chronic infection and persisting inflammation.To date,a large body of research can be reviewed for coatings which potentially prevent bacterial infection while promoting implant integration.Yet only a very small number has been translated from bench to bedside.This study provides an in-depth analysis of the stability,antibacterial mechanism,and biocompatibility of medical grade polycaprolactone(mPCL),coated with human serum albumin(HSA),the most abundant protein in blood plasma,and tannic acid(TA),a natural polyphenol with antibacterial properties.Molecular docking studies demonstrated that HSA and TA interact mainly through hydrogen-bonding,ionic and hydrophobic interactions,leading to smooth and regular assemblies.In vitro bacteria adhesion testing showed that coated scaffolds maintained their antimicrobial properties over 3 days by significantly reducing S.aureus colonization and biofilm formation.Notably,amplitude modulation-frequency modulation(AMFM)based viscoelasticity mapping and transmission electron microscopy(TEM)data suggested that HSA/TA-coatings cause morphological and mechanical changes on the outer cell membrane of s.aureus leading to membrane disruption and cell death while proving non-toxic to human primary cells.These results support this antibiotic-free approach as an effective and biocompatible strategy to prevent biofilm-related biomaterial infections.
