Using a photosensitizer(PS),light,and oxygen,photodynamic therapy creates cytotoxic reactive oxygen species,such as singlet oxygen(1O2),that kill cancer cells.Many cancer cell lines have up to 300 times more folic aci...Using a photosensitizer(PS),light,and oxygen,photodynamic therapy creates cytotoxic reactive oxygen species,such as singlet oxygen(1O2),that kill cancer cells.Many cancer cell lines have up to 300 times more folic acid receptors than healthy cells.Therefore,folic acid is often used to improve selectivity of PSs.Photobleaching poses a disadvantage for PSs.In this paper,we have studied the photoinduced changes of meso-substituted cationic pyridyl porphyrins in the presence of folic acid using uorescence and absorption spectroscopy.In this work,it was demonstrated that L-histidine,which is a 1O2 quencher,and D-mannitol,which is a hydroxyl radical quencher,can reduce photobleaching of cationic porphyrins and their interaction products with FA.This implies both singlet oxygen and hydroxyl radicals are involved in photobleaching.Additionally,our study revealed certain important features of the photobleaching of cationic porphyrins in the presence of folic acid.展开更多
Different mechanisms of laser radiation influence on the chemical transport inside a biotissue are investigated. The average size of speckles existing inside a biotissue and the radiation intensity in them are estimat...Different mechanisms of laser radiation influence on the chemical transport inside a biotissue are investigated. The average size of speckles existing inside a biotissue and the radiation intensity in them are estimated experimentally. Gradient forces and forces caused by the thermal expansion acting on the micro-volume inside a tissue as result of an irregular light field are calculated at different average light intensities including therapeutic. Using theoretical simulation taking into account nonequilibrium light absorption in structural elements of biotissue it has been obtained that the coherent light influences on the chemical transport more strongly in comparison with the incoherent one.展开更多
基金supported by the RA MESCS Science Committee and Belarusian Republican Foundation for Fundamental Research in the frames of the joint research project SC No.21SC-BRFFR-1F007 and BRFFR Grant No.21ARM-014 accordingly,as well as from the Ministry of Science and Higher Education of Russian Federation within the framework of a state assignment(project No.FSRR-2023-0007)。
文摘Using a photosensitizer(PS),light,and oxygen,photodynamic therapy creates cytotoxic reactive oxygen species,such as singlet oxygen(1O2),that kill cancer cells.Many cancer cell lines have up to 300 times more folic acid receptors than healthy cells.Therefore,folic acid is often used to improve selectivity of PSs.Photobleaching poses a disadvantage for PSs.In this paper,we have studied the photoinduced changes of meso-substituted cationic pyridyl porphyrins in the presence of folic acid using uorescence and absorption spectroscopy.In this work,it was demonstrated that L-histidine,which is a 1O2 quencher,and D-mannitol,which is a hydroxyl radical quencher,can reduce photobleaching of cationic porphyrins and their interaction products with FA.This implies both singlet oxygen and hydroxyl radicals are involved in photobleaching.Additionally,our study revealed certain important features of the photobleaching of cationic porphyrins in the presence of folic acid.
文摘Different mechanisms of laser radiation influence on the chemical transport inside a biotissue are investigated. The average size of speckles existing inside a biotissue and the radiation intensity in them are estimated experimentally. Gradient forces and forces caused by the thermal expansion acting on the micro-volume inside a tissue as result of an irregular light field are calculated at different average light intensities including therapeutic. Using theoretical simulation taking into account nonequilibrium light absorption in structural elements of biotissue it has been obtained that the coherent light influences on the chemical transport more strongly in comparison with the incoherent one.
