A new alpha-pyrone derivative, epimedokoreanone A was isolated from the aerial parts of Epimedium koreanum Nakai. Its structure was determined by spectroscopic methods (IR, MS, H-1 NMR, C-13 NMR, DEPT, H-1-C-13 COSY a...A new alpha-pyrone derivative, epimedokoreanone A was isolated from the aerial parts of Epimedium koreanum Nakai. Its structure was determined by spectroscopic methods (IR, MS, H-1 NMR, C-13 NMR, DEPT, H-1-C-13 COSY and H-1-C-13 long range COSY)展开更多
Two new sesquiterpenes were isolated from the rhizomes of Ligularia intermedia. Their structures were determined as eremophil-7(8)-en-12,8(4 beta, 6 alpha)-diolide(1) and eremophil-7(8)-en-12,8(4 beta, 6 beta)-diolide...Two new sesquiterpenes were isolated from the rhizomes of Ligularia intermedia. Their structures were determined as eremophil-7(8)-en-12,8(4 beta, 6 alpha)-diolide(1) and eremophil-7(8)-en-12,8(4 beta, 6 beta)-diolide(2) by spectroscopic methods.展开更多
Two homogeneous new peptidoglycans were obtained from Lycium barbarum L. Theywere found to be effective ingredients capable of resining lipid peroxidation. The componentsand linkages of the two homogeneous polysacchar...Two homogeneous new peptidoglycans were obtained from Lycium barbarum L. Theywere found to be effective ingredients capable of resining lipid peroxidation. The componentsand linkages of the two homogeneous polysaccharides were studied by means of complete acidhydrolysis, periodate oxidation. Smith degradation enzyme hydrolysis, IR, GC and aminoacid analysis. Homogeneous polysaccharide LBPC2 was found to be a β(1→4)(1→6) peptidoglycanwith MW of 1.2×104, composed of Xyl, Rha. Man in a molar ratio of8.8:2.3;1, LBPC4 was foundto be a a(1→4)(1→6) peptidoglycan with MW of 1.0×104, composed of glucans.展开更多
Synthesis of the protected tetrasaccharide II is reported as the key intermediate of tetrasaccharide from laminin that is an important glycoprotein on the basement membrane.
Spin labeled PNAT(10) was synthesized by solid phase method with Boc strategy. The product was characterized by ESR and TOF MS analysis revealing a facile spin labeling of PNA by in situ solid phase synthesis.
To investigate the feasibility of intramuscular electro-acupuncture-mediated VEGF gene transfer on experimental limb ischemia, the transcription and expression of VEGF gene have been detected with RT-PCR and immunohis...To investigate the feasibility of intramuscular electro-acupuncture-mediated VEGF gene transfer on experimental limb ischemia, the transcription and expression of VEGF gene have been detected with RT-PCR and immunohistochemistry staining, and the biological effect of VEGF gene inside the nat limb has been observed through vascular angiography. Results from angiography show that the transfer of VEGF gene into the rat with experimental limb ischemia can stimulate the formation of neovessel, establish collateral circulation, augment blood perfusion, and ameliorate distal limb necrosis. These findings reveal that intramuscular electro acupuncture-mediated VEGF gene transfer may present a new therapeutic modality for treating tissue ischemia.展开更多
The effects of lanthanides on the hydrolysis of phosphatidylinositol in human erythrocyte membranes were studied. 3H-inositoI labeling chromatography and HPLC were used to determine inositol 1, 4, 5-triphosphatc and d...The effects of lanthanides on the hydrolysis of phosphatidylinositol in human erythrocyte membranes were studied. 3H-inositoI labeling chromatography and HPLC were used to determine inositol 1, 4, 5-triphosphatc and dia-cylglycerol separately, the hydrolytic products of phosphatidylinositol due to the reaction of lanthanide ions with human erythrocyte membranes. The unhydrolyzed phosphatidylinositol in membranes was also determined. The results indicate that the hydrolysis of phosphatidylinositol can be promoted by lanthanides (La3+, Ce3+, Y3+, Tb3+) and the effects of La3+ and Ce3+ are stronger than those of Y3+ and Tb3+.展开更多
Six kinds of chemokines including SDF-1α, IP-10, KG, RANTES, MCP-1 and Lymphotactin have been amplified using the primers designed by ourselves with RT-PCR from the total RNA of a medullary-type murine thymic epithel...Six kinds of chemokines including SDF-1α, IP-10, KG, RANTES, MCP-1 and Lymphotactin have been amplified using the primers designed by ourselves with RT-PCR from the total RNA of a medullary-type murine thymic epithelial cell line MTEC1 cells. The amplified cDNA fragments of the chemokines have been identified by nucleotide sequencing and Northern blot analysis. The results demonstrate that there are two different splicing forms of SDF-1 mRNA existing in MTEC1. The concentrated culture supernatant of MTEC1 shows an intermediate level of chemotaxis to CD4-CD8- thymocytes, which conforms to the biological function of SDF-1.展开更多
Cell-to-cell and cell-to-extracellular matrix (ECM) interactions in the functions of cell adhesion and signal transduction are important in global control of cell phenotypes and cell behavior and are crucial for maint...Cell-to-cell and cell-to-extracellular matrix (ECM) interactions in the functions of cell adhesion and signal transduction are important in global control of cell phenotypes and cell behavior and are crucial for maintenance of homeostasis and structural/functional stabilization of tissues and organs. Cell adhesion receptors are recognized as the molecular basis of cell adhesion. Cadherin and lntegrin are widely expressed adhesion receptors in most tissues. They are transmembrane glycoproteins which,through their cytoplasmic domain, bind to many proteins at the inner surface of cell membrane to form molecule-linkage complexes and then connect with the cytoskeleton. Through cell adhesion receptors a network functioning as cell adhesion and signal transduction is organized between tissue cells and cellECM. In this regard cell adhesion receptors play an important role in regulation of morphogenesis, cellcell recognition, cell migration, cell sorting and the determination of cell’s fate in development.展开更多
There exists significant individual variation in electroacupuncture (EA) induced analgesia.Rats may be classified as high- and low-responders according to their response to EA analgesia. The content of cholecystokinin...There exists significant individual variation in electroacupuncture (EA) induced analgesia.Rats may be classified as high- and low-responders according to their response to EA analgesia. The content of cholecystokinin octapeptide like immunoreactivity (CCK-8-ir) in the spinal perfusate of highresponder (HR) and low-responder (LR) rats was measured with radioimmunoassay. The result showed that the CCK-8-ir content in LR rats was significantly lower than that in HR rats. There exists a negative correlation between the EA effect and the CCK-8-ir content during EA stimulation. It is concluded that the spinal release of CCK-8 may be one of the key factors determining the effect of EA analgesia.展开更多
The compositions of the secondary structures of protein in the human breast normal, hyperplasia, fibroadenoma and invasive ductal carcinoma tissues have been estimated from the Fourier self deconvolved spectra, the se...The compositions of the secondary structures of protein in the human breast normal, hyperplasia, fibroadenoma and invasive ductal carcinoma tissues have been estimated from the Fourier self deconvolved spectra, the second derivative spectra and the curve-fitting analysis of the amide I bands in their spectra. Some parameters of the secondary structures of proteins in these 4 types of tissues are significantly different and located in separate ranges.展开更多
The CT technique was first applied to observing the nasal cavity of Ikechosaurus sunailinae. The results indicate that the nasal cavity is very complex, and it was divided into two parts: the posterior-upper part and ...The CT technique was first applied to observing the nasal cavity of Ikechosaurus sunailinae. The results indicate that the nasal cavity is very complex, and it was divided into two parts: the posterior-upper part and the anterior-lower one. These control olfaction and ther-展开更多
文摘A new alpha-pyrone derivative, epimedokoreanone A was isolated from the aerial parts of Epimedium koreanum Nakai. Its structure was determined by spectroscopic methods (IR, MS, H-1 NMR, C-13 NMR, DEPT, H-1-C-13 COSY and H-1-C-13 long range COSY)
文摘Two new sesquiterpenes were isolated from the rhizomes of Ligularia intermedia. Their structures were determined as eremophil-7(8)-en-12,8(4 beta, 6 alpha)-diolide(1) and eremophil-7(8)-en-12,8(4 beta, 6 beta)-diolide(2) by spectroscopic methods.
文摘Two homogeneous new peptidoglycans were obtained from Lycium barbarum L. Theywere found to be effective ingredients capable of resining lipid peroxidation. The componentsand linkages of the two homogeneous polysaccharides were studied by means of complete acidhydrolysis, periodate oxidation. Smith degradation enzyme hydrolysis, IR, GC and aminoacid analysis. Homogeneous polysaccharide LBPC2 was found to be a β(1→4)(1→6) peptidoglycanwith MW of 1.2×104, composed of Xyl, Rha. Man in a molar ratio of8.8:2.3;1, LBPC4 was foundto be a a(1→4)(1→6) peptidoglycan with MW of 1.0×104, composed of glucans.
文摘Synthesis of the protected tetrasaccharide II is reported as the key intermediate of tetrasaccharide from laminin that is an important glycoprotein on the basement membrane.
文摘Spin labeled PNAT(10) was synthesized by solid phase method with Boc strategy. The product was characterized by ESR and TOF MS analysis revealing a facile spin labeling of PNA by in situ solid phase synthesis.
文摘To investigate the feasibility of intramuscular electro-acupuncture-mediated VEGF gene transfer on experimental limb ischemia, the transcription and expression of VEGF gene have been detected with RT-PCR and immunohistochemistry staining, and the biological effect of VEGF gene inside the nat limb has been observed through vascular angiography. Results from angiography show that the transfer of VEGF gene into the rat with experimental limb ischemia can stimulate the formation of neovessel, establish collateral circulation, augment blood perfusion, and ameliorate distal limb necrosis. These findings reveal that intramuscular electro acupuncture-mediated VEGF gene transfer may present a new therapeutic modality for treating tissue ischemia.
基金the National Natural Science Foundation of China (Grant No. 29890280).
文摘The effects of lanthanides on the hydrolysis of phosphatidylinositol in human erythrocyte membranes were studied. 3H-inositoI labeling chromatography and HPLC were used to determine inositol 1, 4, 5-triphosphatc and dia-cylglycerol separately, the hydrolytic products of phosphatidylinositol due to the reaction of lanthanide ions with human erythrocyte membranes. The unhydrolyzed phosphatidylinositol in membranes was also determined. The results indicate that the hydrolysis of phosphatidylinositol can be promoted by lanthanides (La3+, Ce3+, Y3+, Tb3+) and the effects of La3+ and Ce3+ are stronger than those of Y3+ and Tb3+.
文摘Six kinds of chemokines including SDF-1α, IP-10, KG, RANTES, MCP-1 and Lymphotactin have been amplified using the primers designed by ourselves with RT-PCR from the total RNA of a medullary-type murine thymic epithelial cell line MTEC1 cells. The amplified cDNA fragments of the chemokines have been identified by nucleotide sequencing and Northern blot analysis. The results demonstrate that there are two different splicing forms of SDF-1 mRNA existing in MTEC1. The concentrated culture supernatant of MTEC1 shows an intermediate level of chemotaxis to CD4-CD8- thymocytes, which conforms to the biological function of SDF-1.
文摘Cell-to-cell and cell-to-extracellular matrix (ECM) interactions in the functions of cell adhesion and signal transduction are important in global control of cell phenotypes and cell behavior and are crucial for maintenance of homeostasis and structural/functional stabilization of tissues and organs. Cell adhesion receptors are recognized as the molecular basis of cell adhesion. Cadherin and lntegrin are widely expressed adhesion receptors in most tissues. They are transmembrane glycoproteins which,through their cytoplasmic domain, bind to many proteins at the inner surface of cell membrane to form molecule-linkage complexes and then connect with the cytoskeleton. Through cell adhesion receptors a network functioning as cell adhesion and signal transduction is organized between tissue cells and cellECM. In this regard cell adhesion receptors play an important role in regulation of morphogenesis, cellcell recognition, cell migration, cell sorting and the determination of cell’s fate in development.
文摘There exists significant individual variation in electroacupuncture (EA) induced analgesia.Rats may be classified as high- and low-responders according to their response to EA analgesia. The content of cholecystokinin octapeptide like immunoreactivity (CCK-8-ir) in the spinal perfusate of highresponder (HR) and low-responder (LR) rats was measured with radioimmunoassay. The result showed that the CCK-8-ir content in LR rats was significantly lower than that in HR rats. There exists a negative correlation between the EA effect and the CCK-8-ir content during EA stimulation. It is concluded that the spinal release of CCK-8 may be one of the key factors determining the effect of EA analgesia.
文摘The compositions of the secondary structures of protein in the human breast normal, hyperplasia, fibroadenoma and invasive ductal carcinoma tissues have been estimated from the Fourier self deconvolved spectra, the second derivative spectra and the curve-fitting analysis of the amide I bands in their spectra. Some parameters of the secondary structures of proteins in these 4 types of tissues are significantly different and located in separate ranges.
文摘The CT technique was first applied to observing the nasal cavity of Ikechosaurus sunailinae. The results indicate that the nasal cavity is very complex, and it was divided into two parts: the posterior-upper part and the anterior-lower one. These control olfaction and ther-
