Methoxyl poly (ethylene glycol) (mPEG-OH) was successfully grafted onto branched polyethyleneimine (hy-PEI) to yield a water soluble graft copolymer mPEG-g-PEI. This copolymer may package super- paramagnetic iron oxid...Methoxyl poly (ethylene glycol) (mPEG-OH) was successfully grafted onto branched polyethyleneimine (hy-PEI) to yield a water soluble graft copolymer mPEG-g-PEI. This copolymer may package super- paramagnetic iron oxide (SPIO) by ligand exchange. The SPIO weight percentage in the polymer coated nanoparticles was determined to be 55%, the size and zeta potential of nanoparticles was 50 nm and 12 mV respectively. Antibody fixation onto the complex (mPEI-g-PEG-SPIO) surface layer was achieved by activated single chain monoclonal antibody against prostate stem cell antigen (PSCA). Our study showed that the single chain antibody functionalized nanoprobe (scAbPSCA-PEI-g-PEG-SPIO) with a small size can specifically enter the prostate cancer cells, decreasing MRI T2-weighted signal intensity of prostate cancer cells to 44.76%. Our results revealed that the potential of this magnetic nanoparticulate system promised as a novel MRI nanoprobe for early diagnosis of prostate cancer (PCa).展开更多
基金Supported by the National Natural Science Foundation of China (Grant Nos. 30772178, 50673103)Natural Science Foundation of Guangdong Province (Grant Nos. 7117362, 7003703)Key Clinical Program of Ministry of Health Foundation of China
文摘Methoxyl poly (ethylene glycol) (mPEG-OH) was successfully grafted onto branched polyethyleneimine (hy-PEI) to yield a water soluble graft copolymer mPEG-g-PEI. This copolymer may package super- paramagnetic iron oxide (SPIO) by ligand exchange. The SPIO weight percentage in the polymer coated nanoparticles was determined to be 55%, the size and zeta potential of nanoparticles was 50 nm and 12 mV respectively. Antibody fixation onto the complex (mPEI-g-PEG-SPIO) surface layer was achieved by activated single chain monoclonal antibody against prostate stem cell antigen (PSCA). Our study showed that the single chain antibody functionalized nanoprobe (scAbPSCA-PEI-g-PEG-SPIO) with a small size can specifically enter the prostate cancer cells, decreasing MRI T2-weighted signal intensity of prostate cancer cells to 44.76%. Our results revealed that the potential of this magnetic nanoparticulate system promised as a novel MRI nanoprobe for early diagnosis of prostate cancer (PCa).
