Nerve cell metabolism in post brain ischemia depends on increased microcirculation perfusion and transport function of microvascular endothelial cells. In the present study, a rat model of middle cerebral artery occlu...Nerve cell metabolism in post brain ischemia depends on increased microcirculation perfusion and transport function of microvascular endothelial cells. In the present study, a rat model of middle cerebral artery occlusion was established to investigate the influence of electroacupuncture （EA） on hippocampal CA1 cerebral blood flow and glucose transporter 1 （GLUT1） expression in the microvascular endothelial cells. Following EA at Neiguan （PC 6）, the cerebral blood flow in the ischemic hippocampal CA1 region was significantly elevated, the number and microvascular integrated absorbance of the GLUTl-positive cells were significantly increased, nerve cell damage was ameliorated, and GLUT1 protein expression in the ischemic hippocampus was significantly increased. Results demonstrate that EA increased the cerebral blood flow of the hippocampal CA1 region and improved the glucose transport function, thereby attenuating neuronal injuries.展开更多
Esophageal carcinogenesis is a multi-stage process, involving a variety of changes in gene expression and physiological structure change. MicroRNAs (miRNAs) are a class of small non-coding endogenous RNA molecules. Re...Esophageal carcinogenesis is a multi-stage process, involving a variety of changes in gene expression and physiological structure change. MicroRNAs (miRNAs) are a class of small non-coding endogenous RNA molecules. Recent innovation in miRNAs profiling technology have shed new light on the pathology of esophageal carcinoma (EC), and also heralded great potential for exploring novel biomarkers for both EC diagnosis and treatment. Frequent dysregulation of miRNA in malignancy highlights the study of molecular factors upstream of gene expression following the extensive investigation on elucidating the important role of miRNA in carcinogenesis. We herein present a thorough review of the role of miRNAs in EC, addressing miRNA functions, their putative role as oncogenes or tumor suppressors and their potential target genes. The recent progresses in discovering the quantifiable circulating cancer-associated miRNAs indicate the potential clinical use of miRNAs as novel minimally invasive biomarkers for EC and other cancers. We also discuss the potential role of miRNAs in detection, screening and surveillance of EC as miRNAs can be a potential target in personalized treatment of EC.展开更多
Objective To investigate the effects of the Aconiti lateralis Radix Praeparata-Cinnamomi Cortex (ALRP-CC)compound on the expression of intestinal neurotransmitters and stem cell factor (SCF),c-kitprotooncogeneprotein ...Objective To investigate the effects of the Aconiti lateralis Radix Praeparata-Cinnamomi Cortex (ALRP-CC)compound on the expression of intestinal neurotransmitters and stem cell factor (SCF),c-kitprotooncogeneprotein (c-Kit) in slow transit constipation (STC) rats with Yang deficiency syndrome,and to explore its mechanism of action for improving intestinal motility.展开更多
Histological and physiological studies indicated that most skeletal muscles can be divided into a series of relatively independent sub-volumes: "neuromuscular compartments" and the partitioning property of t...Histological and physiological studies indicated that most skeletal muscles can be divided into a series of relatively independent sub-volumes: "neuromuscular compartments" and the partitioning property of the muscle result in the localization of muscle reflex. In the present experiment we studied the recruitment properties of medial gastracnemius (MG) muscle motoneuron pool of decerebrate cat with two kinds of local mechanical stimulation: local stretch and acupuncture-like stimuli. The results indicate that: (ⅰ) there is an obvious property of localization of recruitment activity, only the MNs which innervate the stimulated compartment were recruited by weaker and shorter stimuli; (ⅱ) recruitment activity spread to those MNs which supply the adjacent and distal compartments during the strength of stimulation or duration of the stimulation was increased; and (ⅲ) the recruitment property of muscle activity elicited by the local mechanical stimulation is thought similar to that of "needle feeling" along the meridian pathway during stimulation of acupuncture point.展开更多
Objective To explore the point specificity of eye-acupuncture and the mechanism of eye-acupuncture on diarrhea-predominant irritable bowel syndrome (D-IBS).Methods Forty male Wistar rats of SPF grade were randomly div...Objective To explore the point specificity of eye-acupuncture and the mechanism of eye-acupuncture on diarrhea-predominant irritable bowel syndrome (D-IBS).Methods Forty male Wistar rats of SPF grade were randomly divided into a normal group,a model group,a eye-acupuncture point(AA) group展开更多
Objective To explore the anti-tumor effect and its mechanism of Sendai virus Tianjing strain defective interfering particles(DIP)on mouse models of colon carcinoma.Methods CT26 cells(5×106/0.1 ml)were subcutaneou...Objective To explore the anti-tumor effect and its mechanism of Sendai virus Tianjing strain defective interfering particles(DIP)on mouse models of colon carcinoma.Methods CT26 cells(5×106/0.1 ml)were subcutaneously injected into the back of Bal B/c mice to establish murine colon carcinoma model.After the tumors reached 5 mm in diameter,the mice were randomly di-展开更多
Objective To observe the effects of excessive fluorideon osteocalcin and glucose metabolism in mice.Methods Thirty-two male C57 mice ( body mass: 18-24 g) were selected as study subjects which were randomizedinto four...Objective To observe the effects of excessive fluorideon osteocalcin and glucose metabolism in mice.Methods Thirty-two male C57 mice ( body mass: 18-24 g) were selected as study subjects which were randomizedinto four groups (8 mice in each group) accordingto their body mass by random number table method:0 mg /L group (control group),50 mg /L fluorine group,100 mg /L fluorine group and 150 mg /L fluorine group.Sodium fluoride in distilled water was freely taken bythese animals to replicate fluorosis animal model. After12 weeks,total osteocalcin,uncarboxylated osteocalcin,insulin and glucagon were measured by enzyme-linkedimmunosorbent assay,fasting blood glucose and glycatedhemoglobin were measured by chemiluminescence.展开更多
Objective To explore the role and potential mechanism of humanα-defensin 1 (HNP-1) on low-density lipoprotein (LDL) oxidation ability of human endothenial cells (EVC304) .Methods Post incubation with LDL for3 h,the m...Objective To explore the role and potential mechanism of humanα-defensin 1 (HNP-1) on low-density lipoprotein (LDL) oxidation ability of human endothenial cells (EVC304) .Methods Post incubation with LDL for3 h,the malondiadehyde (MDA) and protein carbonyl (PCO) were detected in untreated ECV304 (control)展开更多
Objective To investigate the effect of Metformin on host-microbial metabolites in rats with glycolipid metabolism disorders by metabolomics method, and to explain the mechanism of Metformin in improving glycolipid met...Objective To investigate the effect of Metformin on host-microbial metabolites in rats with glycolipid metabolism disorders by metabolomics method, and to explain the mechanism of Metformin in improving glycolipid metabolism.Methods Rats were randomly divided into normal control group(Con), model group(Mod) and Metformin group (Met). Feces were collected from each group. Ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) system was used to quantitate the fecal metabolites in each group.展开更多
Objective To study the effects of high glucose on the proliferation,osteoblast differentiation and glucose metabolism of mouse embryonic osteoblast precursor cells(MC3T3-E1).Methods MC3T3-E1 cells were cultured with g...Objective To study the effects of high glucose on the proliferation,osteoblast differentiation and glucose metabolism of mouse embryonic osteoblast precursor cells(MC3T3-E1).Methods MC3T3-E1 cells were cultured with glucose a-MEM at concentrations of 5.6 mmol/L(Con group)and 25.0 mmol/L(HG group).Cell proliferation was detected by CCK-8 and Ki67 immunofluorescence.展开更多
基金the National Natural Science Foundation of China,No. 30672717
文摘Nerve cell metabolism in post brain ischemia depends on increased microcirculation perfusion and transport function of microvascular endothelial cells. In the present study, a rat model of middle cerebral artery occlusion was established to investigate the influence of electroacupuncture （EA） on hippocampal CA1 cerebral blood flow and glucose transporter 1 （GLUT1） expression in the microvascular endothelial cells. Following EA at Neiguan （PC 6）, the cerebral blood flow in the ischemic hippocampal CA1 region was significantly elevated, the number and microvascular integrated absorbance of the GLUTl-positive cells were significantly increased, nerve cell damage was ameliorated, and GLUT1 protein expression in the ischemic hippocampus was significantly increased. Results demonstrate that EA increased the cerebral blood flow of the hippocampal CA1 region and improved the glucose transport function, thereby attenuating neuronal injuries.
文摘Esophageal carcinogenesis is a multi-stage process, involving a variety of changes in gene expression and physiological structure change. MicroRNAs (miRNAs) are a class of small non-coding endogenous RNA molecules. Recent innovation in miRNAs profiling technology have shed new light on the pathology of esophageal carcinoma (EC), and also heralded great potential for exploring novel biomarkers for both EC diagnosis and treatment. Frequent dysregulation of miRNA in malignancy highlights the study of molecular factors upstream of gene expression following the extensive investigation on elucidating the important role of miRNA in carcinogenesis. We herein present a thorough review of the role of miRNAs in EC, addressing miRNA functions, their putative role as oncogenes or tumor suppressors and their potential target genes. The recent progresses in discovering the quantifiable circulating cancer-associated miRNAs indicate the potential clinical use of miRNAs as novel minimally invasive biomarkers for EC and other cancers. We also discuss the potential role of miRNAs in detection, screening and surveillance of EC as miRNAs can be a potential target in personalized treatment of EC.
文摘Objective To investigate the effects of the Aconiti lateralis Radix Praeparata-Cinnamomi Cortex (ALRP-CC)compound on the expression of intestinal neurotransmitters and stem cell factor (SCF),c-kitprotooncogeneprotein (c-Kit) in slow transit constipation (STC) rats with Yang deficiency syndrome,and to explore its mechanism of action for improving intestinal motility.
文摘Histological and physiological studies indicated that most skeletal muscles can be divided into a series of relatively independent sub-volumes: "neuromuscular compartments" and the partitioning property of the muscle result in the localization of muscle reflex. In the present experiment we studied the recruitment properties of medial gastracnemius (MG) muscle motoneuron pool of decerebrate cat with two kinds of local mechanical stimulation: local stretch and acupuncture-like stimuli. The results indicate that: (ⅰ) there is an obvious property of localization of recruitment activity, only the MNs which innervate the stimulated compartment were recruited by weaker and shorter stimuli; (ⅱ) recruitment activity spread to those MNs which supply the adjacent and distal compartments during the strength of stimulation or duration of the stimulation was increased; and (ⅲ) the recruitment property of muscle activity elicited by the local mechanical stimulation is thought similar to that of "needle feeling" along the meridian pathway during stimulation of acupuncture point.
文摘Objective To explore the point specificity of eye-acupuncture and the mechanism of eye-acupuncture on diarrhea-predominant irritable bowel syndrome (D-IBS).Methods Forty male Wistar rats of SPF grade were randomly divided into a normal group,a model group,a eye-acupuncture point(AA) group
文摘Objective To explore the anti-tumor effect and its mechanism of Sendai virus Tianjing strain defective interfering particles(DIP)on mouse models of colon carcinoma.Methods CT26 cells(5×106/0.1 ml)were subcutaneously injected into the back of Bal B/c mice to establish murine colon carcinoma model.After the tumors reached 5 mm in diameter,the mice were randomly di-
文摘Objective To observe the effects of excessive fluorideon osteocalcin and glucose metabolism in mice.Methods Thirty-two male C57 mice ( body mass: 18-24 g) were selected as study subjects which were randomizedinto four groups (8 mice in each group) accordingto their body mass by random number table method:0 mg /L group (control group),50 mg /L fluorine group,100 mg /L fluorine group and 150 mg /L fluorine group.Sodium fluoride in distilled water was freely taken bythese animals to replicate fluorosis animal model. After12 weeks,total osteocalcin,uncarboxylated osteocalcin,insulin and glucagon were measured by enzyme-linkedimmunosorbent assay,fasting blood glucose and glycatedhemoglobin were measured by chemiluminescence.
文摘Objective To explore the role and potential mechanism of humanα-defensin 1 (HNP-1) on low-density lipoprotein (LDL) oxidation ability of human endothenial cells (EVC304) .Methods Post incubation with LDL for3 h,the malondiadehyde (MDA) and protein carbonyl (PCO) were detected in untreated ECV304 (control)
文摘Objective To investigate the effect of Metformin on host-microbial metabolites in rats with glycolipid metabolism disorders by metabolomics method, and to explain the mechanism of Metformin in improving glycolipid metabolism.Methods Rats were randomly divided into normal control group(Con), model group(Mod) and Metformin group (Met). Feces were collected from each group. Ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) system was used to quantitate the fecal metabolites in each group.
文摘Objective To study the effects of high glucose on the proliferation,osteoblast differentiation and glucose metabolism of mouse embryonic osteoblast precursor cells(MC3T3-E1).Methods MC3T3-E1 cells were cultured with glucose a-MEM at concentrations of 5.6 mmol/L(Con group)and 25.0 mmol/L(HG group).Cell proliferation was detected by CCK-8 and Ki67 immunofluorescence.
