OBJECTIVE: To investigate the effect of thorascopic administration of ginseng polysaccharides(GPS)plus dendritic cells(DC) on T helper cell type 1/T helper cell type 2(Th1/Th2) balance in patients with non-small cell ...OBJECTIVE: To investigate the effect of thorascopic administration of ginseng polysaccharides(GPS)plus dendritic cells(DC) on T helper cell type 1/T helper cell type 2(Th1/Th2) balance in patients with non-small cell lung cancer(NSCLC).METHODS: A total of 96 NSCLC patients were divided evenly into two groups. The control group was treated with DCs alone and the treatment group was treated with DCs plus GPS. After DCs and GPS were administered thoracoscopically, once a week,4 times for 30 days, the patients' quality of life was measured with the Functional Assessment of Can-cer Treatment-Lung(FACT-L) questionnaire before and after treatment. Serum interferon-γ(INF-γ), interleukin-4(IL-4), IL-2 and IL-5 were examined before and after treatments.RESULTS: The level of Th1 cytokines(INF-γ, IL-2)and the ratio of Th1/Th2 cytokines(INF-γ/IL-4, IL-2/IL-5) increased in both treatment groups, while Th2cytokines(IL-4, IL-5) and FACT-L scores decreased(P<0.01). Furthermore, after treatment Th1 cytokines(INF-γ, IL-2) and the ratio of Th1/Th2 cytokines(INF-γ/IL-4, IL-2/IL-5) were higher in the DCs +GPS group than in the control group(P<0.05). Conversely, FACT-L scores and Th2 cytokines(IL-4, IL-5)were higher in the control group than in the DCs +GPS group(P<0.05).CONCLUSION: The treatment regime of DCs plus GPS had a greater effect on NSCLC patients' immune function as compared with DCs alone. This was evident by increased expression of Th1 cytokines(INF-γ, IL-2) and the ratio of Th1/Th2(INF-γ/IL-4, IL-2/IL-5), as well as by decreased FACT-L scores and the expression of Th2 cytokines(IL-4,IL-5).展开更多
Objective： To investigate whether pinching spine （PS, i.e.捏脊, a traditional Chinese manipulativetherapy） is beneficial to ameliorating the depressive state （including behavioral deficit, retardative weight gaina...Objective： To investigate whether pinching spine （PS, i.e.捏脊, a traditional Chinese manipulativetherapy） is beneficial to ameliorating the depressive state （including behavioral deficit, retardative weight gainand decreased sucrose consumption） in a rat model of depression induced by chronic unpredictable stress （CUS）and to explore the candidate mechanism of action. Methods： PS was performed on rats＇ spine once daily for 1week after exposure to CUS. The open-field test, body weight measuring, and sucrose intake test were appliedon different dates： before stress （dO）, at the end of stress （d21） and after PS treatment （d28）, respectively. Thenthe rats＇ hippocampuses were performed genome-wide microarray analysis, and the expression levels of severalgenes were evaluated by real-time polymerase chain reaction （PCR）. Results： Exposure to CUS resulted indecreases of behavioral activity and sucrose consumption, which were reversed significantly after PS treatment.The expression of several genes relevant to energy metabolism, anti-oxidation, and olfactory receptor, etc., weredown-regulated, while the expression of those relevant to hemostasis, immunity-inflammation, and restrictionof activities and ingestion, etc., were up-regulated in hippocampuses of rats exposed to CUS. PS treatmentsignificantly inverted these changes. Furthermore, increase or decrease in gone expression evaluated by real-time PCR was concordant with up-regulated or down-regulated expression evaluated by microarray analysis.Conclusion： PS showed a potential antidepressant-like effect, of which the action mechanism might be due togone expression regulation in hippocampus.展开更多
基金2011 National Key Specialty Construction of Clinical Projects of ChinaThe National Natural Science Foundation of China(No.81273639)Science and Technology Projects of Traditional Chinese Medicine Bureau in Jiangsu Province of China(No.LB13042)
文摘OBJECTIVE: To investigate the effect of thorascopic administration of ginseng polysaccharides(GPS)plus dendritic cells(DC) on T helper cell type 1/T helper cell type 2(Th1/Th2) balance in patients with non-small cell lung cancer(NSCLC).METHODS: A total of 96 NSCLC patients were divided evenly into two groups. The control group was treated with DCs alone and the treatment group was treated with DCs plus GPS. After DCs and GPS were administered thoracoscopically, once a week,4 times for 30 days, the patients' quality of life was measured with the Functional Assessment of Can-cer Treatment-Lung(FACT-L) questionnaire before and after treatment. Serum interferon-γ(INF-γ), interleukin-4(IL-4), IL-2 and IL-5 were examined before and after treatments.RESULTS: The level of Th1 cytokines(INF-γ, IL-2)and the ratio of Th1/Th2 cytokines(INF-γ/IL-4, IL-2/IL-5) increased in both treatment groups, while Th2cytokines(IL-4, IL-5) and FACT-L scores decreased(P<0.01). Furthermore, after treatment Th1 cytokines(INF-γ, IL-2) and the ratio of Th1/Th2 cytokines(INF-γ/IL-4, IL-2/IL-5) were higher in the DCs +GPS group than in the control group(P<0.05). Conversely, FACT-L scores and Th2 cytokines(IL-4, IL-5)were higher in the control group than in the DCs +GPS group(P<0.05).CONCLUSION: The treatment regime of DCs plus GPS had a greater effect on NSCLC patients' immune function as compared with DCs alone. This was evident by increased expression of Th1 cytokines(INF-γ, IL-2) and the ratio of Th1/Th2(INF-γ/IL-4, IL-2/IL-5), as well as by decreased FACT-L scores and the expression of Th2 cytokines(IL-4,IL-5).
文摘Objective： To investigate whether pinching spine （PS, i.e.捏脊, a traditional Chinese manipulativetherapy） is beneficial to ameliorating the depressive state （including behavioral deficit, retardative weight gainand decreased sucrose consumption） in a rat model of depression induced by chronic unpredictable stress （CUS）and to explore the candidate mechanism of action. Methods： PS was performed on rats＇ spine once daily for 1week after exposure to CUS. The open-field test, body weight measuring, and sucrose intake test were appliedon different dates： before stress （dO）, at the end of stress （d21） and after PS treatment （d28）, respectively. Thenthe rats＇ hippocampuses were performed genome-wide microarray analysis, and the expression levels of severalgenes were evaluated by real-time polymerase chain reaction （PCR）. Results： Exposure to CUS resulted indecreases of behavioral activity and sucrose consumption, which were reversed significantly after PS treatment.The expression of several genes relevant to energy metabolism, anti-oxidation, and olfactory receptor, etc., weredown-regulated, while the expression of those relevant to hemostasis, immunity-inflammation, and restrictionof activities and ingestion, etc., were up-regulated in hippocampuses of rats exposed to CUS. PS treatmentsignificantly inverted these changes. Furthermore, increase or decrease in gone expression evaluated by real-time PCR was concordant with up-regulated or down-regulated expression evaluated by microarray analysis.Conclusion： PS showed a potential antidepressant-like effect, of which the action mechanism might be due togone expression regulation in hippocampus.
