目的探讨多团队协作护理预防髋关节置换术患者下肢深静脉血栓的效果。方法随机选取2016年1月～2017年12月我院收治的髋关节置换术患者60例,采用SPSS21.0将入组患者按先后序列号(1～60)随机分为两组:一组常规健康教育组(30例),一组常规...目的探讨多团队协作护理预防髋关节置换术患者下肢深静脉血栓的效果。方法随机选取2016年1月～2017年12月我院收治的髋关节置换术患者60例,采用SPSS21.0将入组患者按先后序列号(1～60)随机分为两组:一组常规健康教育组(30例),一组常规健康教育基础上多团队协作组(多团队协作组,30例),统计分析两组患者下肢深静脉血栓相关知识掌握程度、预防下肢深静脉血栓的健康信念水平、下肢深静脉血栓、关节脱位、下肢肿胀疼痛、肺栓塞发生情况。结果多团队协作组患者的下肢深静脉血栓相关知识掌握程度评分显著高于常规健康教育组(P<0.05);感知疾病的易感性、严重性、健康行为障碍、益处、健康动力、自我效能评分均显著高于常规健康教育组(P<0.05);下肢深静脉血栓发生率为6.7%(2/30),显著低于常规健康教育组的23.3%(7/30)(P<0.05);关节脱位发生率0显著低于常规健康教育组10.0%(3/30)(P<0.05);但两组患者的下肢肿胀疼痛、肺栓塞发生率3.3%(1/30)、0 vs 6.7%(2/30)、3.3%(1/30)之间的差异均不显著(P>0.05)。结论多团队协作能够对髋关节置换术患者下肢深静脉血栓进行有效预防,同时降低患者的关节脱位发生率,值得在临床推广。展开更多
Objective： To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction （通莲汤, TLD） on esophageal carcinoma Eca109 cells. Methods： Ecal09 cells were treated with TLD and its separated for...Objective： To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction （通莲汤, TLD） on esophageal carcinoma Eca109 cells. Methods： Ecal09 cells were treated with TLD and its separated formulae, including the clearing-heat and detoxification formula （Q）, activating-blood and promoting-qi formula （H） and nourishing-yin and blood formula （Z）. Cell proliferation was measured using the 3-（4,5-dimethyl-2- thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide assay, cell morphology was observed using a microscope, the cell cycle was measured using flow cytometry and the activity of the nuclear factor-kappa B （NF- K B） signal pathway was detected by Western blot. Results： The half maximal inhibitory concentrations of TLD, Q and H were 386, 771 and 729 rag/L, respectively. TLD, Q and H significantly inhibited cell proliferation, with 69.43%, 60.84% and 61.90% of treated cells in the G1 phase of the cell cycle. The percentage of cells in S phase increased significantly after treatment with TLD, Q, and H compared with the control group （P〈0.05）, and TLD showed the strongest effect. Z had no influence on the cell cycle compared with the control group （P〉0.05）. Western blot detection indicated slight differences in the inhibition of the NF- K B pathway by the different formulae. TLD formula strongly inhibited IKK β, NF- K B, interleukin-6 and tumor necrosis factor-α expression compared with the control group. Conclusions： TLD inhibited Eca109 cell proliferation by arresting cells in S phase. The possible mechanism might be related to inhibiting the NF- K B transduction cascade. The combination of the herbs found in the three separate formulae, H, Q and Z, work synergistically in TLD to produce the inhibitory effects of TLD treatment on Eca109 proliferation.展开更多
文摘目的探讨多团队协作护理预防髋关节置换术患者下肢深静脉血栓的效果。方法随机选取2016年1月～2017年12月我院收治的髋关节置换术患者60例,采用SPSS21.0将入组患者按先后序列号(1～60)随机分为两组:一组常规健康教育组(30例),一组常规健康教育基础上多团队协作组(多团队协作组,30例),统计分析两组患者下肢深静脉血栓相关知识掌握程度、预防下肢深静脉血栓的健康信念水平、下肢深静脉血栓、关节脱位、下肢肿胀疼痛、肺栓塞发生情况。结果多团队协作组患者的下肢深静脉血栓相关知识掌握程度评分显著高于常规健康教育组(P<0.05);感知疾病的易感性、严重性、健康行为障碍、益处、健康动力、自我效能评分均显著高于常规健康教育组(P<0.05);下肢深静脉血栓发生率为6.7%(2/30),显著低于常规健康教育组的23.3%(7/30)(P<0.05);关节脱位发生率0显著低于常规健康教育组10.0%(3/30)(P<0.05);但两组患者的下肢肿胀疼痛、肺栓塞发生率3.3%(1/30)、0 vs 6.7%(2/30)、3.3%(1/30)之间的差异均不显著(P>0.05)。结论多团队协作能够对髋关节置换术患者下肢深静脉血栓进行有效预防,同时降低患者的关节脱位发生率,值得在临床推广。
基金Supported by the Natural Science Fundation of Hebei Province of China(No.H2013209053)Scientific and Technological Project of Administration of Traditional Chinese Medicine of Hebei Province,China(No.2014185)
文摘Objective： To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction （通莲汤, TLD） on esophageal carcinoma Eca109 cells. Methods： Ecal09 cells were treated with TLD and its separated formulae, including the clearing-heat and detoxification formula （Q）, activating-blood and promoting-qi formula （H） and nourishing-yin and blood formula （Z）. Cell proliferation was measured using the 3-（4,5-dimethyl-2- thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide assay, cell morphology was observed using a microscope, the cell cycle was measured using flow cytometry and the activity of the nuclear factor-kappa B （NF- K B） signal pathway was detected by Western blot. Results： The half maximal inhibitory concentrations of TLD, Q and H were 386, 771 and 729 rag/L, respectively. TLD, Q and H significantly inhibited cell proliferation, with 69.43%, 60.84% and 61.90% of treated cells in the G1 phase of the cell cycle. The percentage of cells in S phase increased significantly after treatment with TLD, Q, and H compared with the control group （P〈0.05）, and TLD showed the strongest effect. Z had no influence on the cell cycle compared with the control group （P〉0.05）. Western blot detection indicated slight differences in the inhibition of the NF- K B pathway by the different formulae. TLD formula strongly inhibited IKK β, NF- K B, interleukin-6 and tumor necrosis factor-α expression compared with the control group. Conclusions： TLD inhibited Eca109 cell proliferation by arresting cells in S phase. The possible mechanism might be related to inhibiting the NF- K B transduction cascade. The combination of the herbs found in the three separate formulae, H, Q and Z, work synergistically in TLD to produce the inhibitory effects of TLD treatment on Eca109 proliferation.
