Objective: To investigate the effect of Radix Glehniae on the migration and invasion abilities of lung cancer cells, and to explore the underlying mechanism. Method: Normal human bronchial cell line 16 HBE and lung ca...Objective: To investigate the effect of Radix Glehniae on the migration and invasion abilities of lung cancer cells, and to explore the underlying mechanism. Method: Normal human bronchial cell line 16 HBE and lung cancer cell line SK-MES-1 were cultured in vitro. Radix Glehniae extract was prepared to treat the two kinds of cells. The proliferation ability of 16 HBE cells was determined by CCK-8 assay. The migration and invasion abilities of SK-MES-1 cells were determined by Transwell and Matrigel assays, respectively. The expression of TIMP2 m RNA in SK-MES-1 cells was detected by fluorescence quantitative PCR(q-PCR). The secretion level of TIMP2 protein by SK-MES-1 cells was measured by ELISA experiment. Results: Radix Glehniae extract caused no toxic effects on 16 HBE cells at the concentrations of 1 mg/ml to 15 mg/ml. Radix Glehniae extract(from 5 mg/ml to 15 mg/ml) significantly inhibited SK-MES-1 cell migration and invasion abilities, as well as upregulated TIMP2 m RNA expression and enhanced TIMP2 secretion by SK-MES-1 cells. Conclusion: Radix Glehniae can inhibit the migration and invasion behaviors of lung cancer cells by upregulating TIMP2 expression and secretion.展开更多
Objective: To investigate the influence of Xanthii Fructuson the expression of small non-coding RNAs(snc RNA) and the malignant behaviors of lung cancer cells. Method: A549 human lung cancer cells were cultured and tr...Objective: To investigate the influence of Xanthii Fructuson the expression of small non-coding RNAs(snc RNA) and the malignant behaviors of lung cancer cells. Method: A549 human lung cancer cells were cultured and treated with different concentrations(5-15 mg/ml) of the ethanol extract of Xanthii Fructus. The expression of mi R-21 and pi RNA55490 was analyzed by Realtime PCR. The proliferation capacity was detected by CCK-8 assay. The anchorage-independent growth capacity was measured by soft agar colony formation experiment. The cell invasion capacity was determined by Matrigel assay. Results: The Xanthii Fructus alcohol extract down-regulated the expression of the oncogenic snc RNAmi R-21, and up-regulated the expression of the tumor suppressive snc RNA pi RNA55490. At the functional level, proliferation, anchorage-independent growth, and invasion capacities of the lung cancer cells were strongly inhibited by the extract treatment. Conclusion: The Chinese herb Xanthii Fructus inhibits the malignant behaviors of lung cancer cells, and the inhibition is largely associated with its ability to change the expression of snc RNAs.展开更多
文摘Objective: To investigate the effect of Radix Glehniae on the migration and invasion abilities of lung cancer cells, and to explore the underlying mechanism. Method: Normal human bronchial cell line 16 HBE and lung cancer cell line SK-MES-1 were cultured in vitro. Radix Glehniae extract was prepared to treat the two kinds of cells. The proliferation ability of 16 HBE cells was determined by CCK-8 assay. The migration and invasion abilities of SK-MES-1 cells were determined by Transwell and Matrigel assays, respectively. The expression of TIMP2 m RNA in SK-MES-1 cells was detected by fluorescence quantitative PCR(q-PCR). The secretion level of TIMP2 protein by SK-MES-1 cells was measured by ELISA experiment. Results: Radix Glehniae extract caused no toxic effects on 16 HBE cells at the concentrations of 1 mg/ml to 15 mg/ml. Radix Glehniae extract(from 5 mg/ml to 15 mg/ml) significantly inhibited SK-MES-1 cell migration and invasion abilities, as well as upregulated TIMP2 m RNA expression and enhanced TIMP2 secretion by SK-MES-1 cells. Conclusion: Radix Glehniae can inhibit the migration and invasion behaviors of lung cancer cells by upregulating TIMP2 expression and secretion.
文摘Objective: To investigate the influence of Xanthii Fructuson the expression of small non-coding RNAs(snc RNA) and the malignant behaviors of lung cancer cells. Method: A549 human lung cancer cells were cultured and treated with different concentrations(5-15 mg/ml) of the ethanol extract of Xanthii Fructus. The expression of mi R-21 and pi RNA55490 was analyzed by Realtime PCR. The proliferation capacity was detected by CCK-8 assay. The anchorage-independent growth capacity was measured by soft agar colony formation experiment. The cell invasion capacity was determined by Matrigel assay. Results: The Xanthii Fructus alcohol extract down-regulated the expression of the oncogenic snc RNAmi R-21, and up-regulated the expression of the tumor suppressive snc RNA pi RNA55490. At the functional level, proliferation, anchorage-independent growth, and invasion capacities of the lung cancer cells were strongly inhibited by the extract treatment. Conclusion: The Chinese herb Xanthii Fructus inhibits the malignant behaviors of lung cancer cells, and the inhibition is largely associated with its ability to change the expression of snc RNAs.
