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Nonlinear optical imaging by detection with optical parametric amplification(invited paper) 被引量:1
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作者 Yi Sun Haohua Tu Stephen A.Boppart 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS CSCD 2023年第1期5-14,共10页
Nonlinear optical imaging is a versatile tool that has been proven to be exceptionally useful in various research fields.However,due to the use of photomultiplier tubes(PMTs),the wide application of nonlinear optical ... Nonlinear optical imaging is a versatile tool that has been proven to be exceptionally useful in various research fields.However,due to the use of photomultiplier tubes(PMTs),the wide application of nonlinear optical imaging is limited by the incapability of imaging under am-bient light.In this paper,we propose and demonstrate a new optical imaging detection method based on optical parametric amplification(OPA).As a nonlinear optical process,OPA in-trinsically rejects ambient light photons by coherence gating.Periodical poled lithium niobate(PPLN)crystals are used in this study as the media for OPA.Compared to bulk nonlinear optical crystals,PPLN crystals support the generation of OPA signal with lower pump power.Therefore,this characteristic of PPLN crystals is particularly beneficial when using high-repetition-rate lasers,which facilitate high-speed optical signal detection,such as in spec-troscopy and imaging.A PPLN-based OPA system was built to amplify the emitted imaging signal from second harmonic generation(SHG)and coherent anti-Stokes Raman scattering(CARS)microscopy imaging,and the amplified optical signal was strong enough to be detected by a biased photodiode under ordinary room light conditions.With OPA detection,ambient-light-on SHG and CARS imaging becomes possible,and achieves a similar result as PMT detection under strictly dark environments.These results demonstrate that OPA can be used as a substitute for PMTs in nonlinear optical imaging to adapt it to various applications with complex.light ing conditions. 展开更多
关键词 Nonlinear optical microscopy optical parametric amplification optical detection
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Relationships between Features of EmergingAdulthood, Situated Decisions toward PhysicalActivity, and Physical Activity among CollegeStudents: The Moderating Role of ExerciseIntensity Tolerance
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作者 Jinghua Chen Zihe Wang +5 位作者 Fabian Herold Alyx Taylor Jin Kuang Ting Wang Arthur F.Kramer Liye Zou 《International Journal of Mental Health Promotion》 2023年第11期1209-1217,共9页
A significant portion of emerging adults do not achieve recommended levels of physical activity (PA). Previous studies observedassociations between features of emerging adulthood and PA levels, while the potential psy... A significant portion of emerging adults do not achieve recommended levels of physical activity (PA). Previous studies observedassociations between features of emerging adulthood and PA levels, while the potential psychological mechanisms that mightexplain this phenomenon are not fully understood. In this context, there is some evidence that situated decisions towardphysical activity (SDPA) and exercise-intensity tolerance might influence PA level. To provide empirical support for thisassumption, the current study investigated whether (i) features of emerging adulthood are linked to SDPA, which, in turn,might affect PA engagement;(ii) exercise-intensity tolerance moderate the relationship between SDPA and PA level;and (iii)SDPA is a mediator of the relationship between features of emerging adulthood and PA levels under the prerequisite thatexercise-intensity tolerance moderates the link between SDPA and PA engagement. In this study a group of 1,706 Chinesecollege students was recruited and asked to complete a set of questionnaires assessing their SDPA, PA levels, exercise-intensitytolerance, and features associated with emerging adulthood, namely Self-exploration, Instability, and Possibility. Our resultsindicated that SDPA positively predicted PA levels and this relationship became stronger when exercise-intensity tolerance wasused as a moderator. Furthermore, it was observed that individuals with a higher level of Instability and a lower level ofPossibility during emerging adulthood exhibited a lower level of SDPA. Taken together, the results of our study providefurther insights on a potential psychological mechanism linking features of emerging adulthood and physical activity. 展开更多
关键词 Emerging adulthood situated decisions physical activity exercise-intensity tolerance moderated model
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Validity and Reliability of the Preference for and Tolerance of the Intensity of Exercise Questionnaire among Chinese College Students
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作者 Ting Wang Jin Kuang +5 位作者 Fabian Herold Alyx Taylor Sebastian Ludyga Zhihao Zhang Arthur FKramer Liye Zou 《International Journal of Mental Health Promotion》 2023年第1期127-138,共12页
The rising prevalence of physical inactivity is in all age groups(e.g.,in college students)a major public health issue as not meeting the recommended minimum amount of regular physical activity is linked to adverse he... The rising prevalence of physical inactivity is in all age groups(e.g.,in college students)a major public health issue as not meeting the recommended minimum amount of regular physical activity is linked to adverse health events.Vice versa,there is mounting evidence that achieving the recommended amount of regular physical activity is a vital element to prevent chronic diseases,but there is often an insufficient adherence to planned and structured forms of physical activity(i.e.,physical exercises).In this context,there is a large body of evidence indicating that exercise adherence is,among other factors,influenced by exercise-related affective responses.The Preference for and Tolerance of the Intensity of Exer-cise Questionnaire(PRETIE-Q)has been developed to measure these affective responses(e.g.,to specific exercise inten-sities).So far,no validated Chinese version of the PRETIE-Q has been available in the literature.To address this gap in literature,the present study developed and validated a Chinese version of the PRETIE-Q in a large sample of Chinese college students.Data from a total of 1117 college students were collected for analyses concerning factorial validity and construct validity.The re-test reliability was established using a sample of 150 randomly selected participants.In addition,the level of regular physical activity(PA),cardiorespiratoryfitness(CRF),and resilience were used to examine possible links with two domains(preference and tolerance)of the PRETIE-Q.Our results showed that a Chinese version of the PRETIE-Q has a goodfit and reliability(Cronbach’sαof 0.72 to 0.85 for preference and tolerance,respectively;ICC:r=0.72 of preference and r=0.67 of tolerance;fit indices:χ^(2)=21.612,df=19,p>0.05,TLI=0.997,CFI=0.998,RMSEA=0.016,SRMR=0.024).Secondly,positive associations of intensity-tolerance with PA,CRF,and resilience were observed.In summary,this study indicates that the Chinese version of the PRETIE-Q has sound psychometric properties and can be used in Chinese college students.The adapted version of the PRETIE-Q paves the way for further research on exer-cise-related affective responses in Chinese-speaking samples,although the generalizability of ourfindings needs to be established for other cohorts such as adolescents and older people with and without chronic diseases. 展开更多
关键词 PRETIE-Q PLEASURE INTENSITY tolerance PREFERENCE validity
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A Bifactor Analysis Approach to Construct Validity and Reliability of the Affective Exercise Experience Questionnaire among Chinese College Students
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作者 Ting Wang Markus Gerber +5 位作者 Fabian Herold Joseph Bardeen Sebastian Ludyga Alyx Taylor Arthur FKramer Liye Zou 《International Journal of Mental Health Promotion》 2023年第9期995-1008,共14页
Affective exercise experience as an emerging theoretical concept has great potential to provide a more nuanced understanding of individual factors that influence exercise behavior.However,concerning the Affective Exer... Affective exercise experience as an emerging theoretical concept has great potential to provide a more nuanced understanding of individual factors that influence exercise behavior.However,concerning the Affective Exercise Experiences(AFFEXX)questionnaire,it has not been examined yet whether the structural score of the AFFEXX is a useful index to predict physical activity(refers to any bodily movement produced by skeletal muscles that requires energy expenditure).Furthermore,there is currently a gap in knowledge regarding the psychological mechanisms that can explain the relationship between affective exercise experiences and the level of physical activity(PA).In order to adress these gaps in the literature,we conducted two studies among Chinese collge students that aimed(i)to investigated whether the total score of the three AFFEXX-C constructs(antecedent appraisals,core affective exercise experiences,and attraction-antipathy towards exercise)is a relaible indicator that can be utilized in research and pratical settings and(ii)to evalute the specific psychological mechanisms that can explain the relationship between affective exercise experience and PA.In Study 1,we recruited 801 voluntary Chinese college students for bifactor and correlational analyses.In Study 2,875 Chinese college students were enrolled to verify our findings from Study 1 and to explore the aforementioned mechanism.Results from the bifactor analyses supported our hypothesis that the total scores of the three AFFEXX-C constructs can be used among Chinese college students to establish a link with PA.Additionally,our results suggested that core affective exercise experiences and attraction-antipathy mediated the relationship between antecedent appraisals and the level of moderate-to-vigorous intensity PA.Therefore,measuring affective exercise experiences using the AFFEXX-C,specifically the total scores of each individual construct may be a useful approach to predict future PA levels. 展开更多
关键词 Affective exercise experiences bifactor analysis Chinese college students mediation model
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MXene ink hosting zinc anode for high performance aqueous zinc metal batteries
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作者 Jae Min Park Milan Jana +9 位作者 Sang Ha Baek Taehun Kang Peixun Xiong Jeong Hee Park Jun Soo Kim Ali Shayesteh Zeraati Mikhail Shekhirev Paul V.Braun Yury Gogotsi Ho Seok Park 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2023年第1期187-194,I0005,共9页
Despite the safety,low cost,and high theoretical capacity(820 mA h g^(-1))of Zn metal anodes,the practical application of aqueous Zn metal batteries remains a critical challenge due to the Zn dendrite growth,corrosion... Despite the safety,low cost,and high theoretical capacity(820 mA h g^(-1))of Zn metal anodes,the practical application of aqueous Zn metal batteries remains a critical challenge due to the Zn dendrite growth,corrosion,and hydrogen evolution reaction.Herein,we demonstrate the MXene ink hosting Zn metal anodes(MX@Zn)for high-performance and patternable Zn metal full batteries.The as-designed MX@Zn electrode is more facile and reversible than bare Zn and CC@Zn,as verified by better cyclic stability and lower overpotentials of symmetric cells with the plating capacity of 0.05 mA h cm^(-2)at 0.1 m A cm^(-2)and of 1 m A h cm^(-2)at 1 m A cm^(-2).The MX@Zn|MnO_(2)full cells deliver a high specific capacity of 281.9 m A h g^(-1),91.5%of the theoretical capacity,achieving 50%capacity retention from 60 mA g^(-1)to 300 mA g^(-1)and 79.7%of initial capacity after 200 cycles.Moreover,the patterned devices based on the MX@Zn electrode achieve high energy and power densities of 348.57 Wh kg^(-1)and 1556 W kg^(-1),respectively,along with a capacity retention of 64%and Coulombic efficiency of 99%over 500 cycles.The high performance of MX@Zn is attributed to the high electrical conductivity and hydrophilicity of MXene and rapid ion diffusion through the 3D interconnected porous channels. 展开更多
关键词 MXene ink Zinc anode Metal batteries PATTERNING ELECTRODEPOSITION
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The Relationship between Dimensions of Emerging Adulthood and Behavioral Problems among Chinese Emerging Adults: The Mediating Role of Physical Activity and Self- Control
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作者 Jin Kuang Jeffrey Jensen Arnett +9 位作者 Erle Chen Zsolt Demetrovics Fabian Herold Rebecca Y.M.Cheung Daniel L.Hall Michaela Markwart Markus Gerber Sebastian Ludyga Arthur F.Kramer Liye Zou 《International Journal of Mental Health Promotion》 2023年第8期937-948,共12页
Emerging adulthood(EA)is a critical stage of life to develop and sustain a healthy lifestyle,which is also a time of vulnerability to poor physical and mental health outcomes.In this study,we conducted a path analysis... Emerging adulthood(EA)is a critical stage of life to develop and sustain a healthy lifestyle,which is also a time of vulnerability to poor physical and mental health outcomes.In this study,we conducted a path analysis(N=1326)to examine associations among four dimensions of EA,levels of regular physical activity(PA),self-control,MPA tendency and irrational procrastination.Results found:1)higher levels of PA predicted both MPA tendency(β=−0.08,95%CI:−0.11 to−0.06,p<0.001)and irrational procrastination(β=−0.01,95%CI:−0.17 to−0.008,p<0.01)indirectly via self-control;2)Instability(β=0.13,95%CI:0.08 to 0.18,p<0.01)and Responsibility(β=−0.06,95%CI:−0.10 to−0.08,p=0.03)exerted direct effects on irrational procrastination and Instability also indirectly predicted irrational procrastination via MPA tendency(β=0.03,95%CI:0.02 to 0.05,p<0.01).These findings proved that perceived features of EA are linked to behavioral problems and supported that regular PA plays a crucial role to protect mental health. 展开更多
关键词 Emerging adulthood Chinese culture physical activity mobile phone addiction irrational procrastination SELF-CONTROL
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Associations between meeting 24-hour movement guidelines and quality of life among children and adolescents with autism spectrum disorder
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作者 Chuidan Kong Aiguo Chen +9 位作者 Sebastian Ludyga Fabian Herold Sean Healy Mengxian Zhao Alyx Taylor Notger G.Muller Arthur F.Kramer Sitong Chen Mark S.Tremblay Liye Zou 《Journal of Sport and Health Science》 SCIE CSCD 2023年第1期73-86,共14页
Background:The Canadian 24-hour movement behavior(24-HMB)guidelines suggest that a limited amount of screen time use,an adequate level of physical activity(PA),and sufficient sleep duration are beneficial for ensuring... Background:The Canadian 24-hour movement behavior(24-HMB)guidelines suggest that a limited amount of screen time use,an adequate level of physical activity(PA),and sufficient sleep duration are beneficial for ensuring and optimizing the health and quality of life(QoL)of children and adolescents.However,this topic has yet to be examined for children and adolescents with autism spectrum disorder(ASD)specifically.The aim of this cross-sectional observational study was to examine the associations between meeting 24-HMB guidelines and several QoLrelated indicators among a national sample of American children and adolescents with ASD.Methods:Data were taken from the 2020 U.S.National Survey of Children’s Health dataset.Participants(n=956)aged 617 years and currently diagnosed with ASD were included.The exposure of interest was adherence to the 24-HMB guidelines.Outcomes were QoL indicators,including learning interest/curiosity,repeating grades,adaptive ability,victimization by bullying,and behavioral problems.Categorical variables were described with unweighted sample counts and weighted percentages.Age,sex,race,preterm birth status,medication,behavioral treatment,household poverty level,and the educational level of the primary caregivers were included as covariates.Odds ratio(OR)and 95%confidence interval(95%CI)were used to present the strength of association between adherence to 24-HMB guidelines and QoL-related indicators.Results:Overall,452 participants(45.34%)met 1 of the 3 recommendations,216(22.65%)met 2 recommendations,whereas only 39 participants(5.04%)met all 3 recommendations.Compared with meeting none of the recommendations,meeting both sleep duration and PA recommendations(OR=3.92,95%CI:1.639.48,p<0.001)or all 3 recommendations(OR=2.11,95%CI:1.034.35,p=0.04)was associated with higher odds of showing learning interest/curiosity.Meeting both screen time and PA recommendations(OR=0.15,95%CI:0.040.61,p<0.05)or both sleep duration and PA recommendations(OR=0.24,95%CI:0.070.87,p<0.05)was associated with lower odds of repeating any grades.With respect to adaptive ability,participants who met only the PA recommendation of the 24-HMB were less likely to have difficulties dressing or bathing(OR=0.11,95%CI:0.020.66,p<0.05)than those who did not.For participants who met all 3 recommendations(OR=0.38,95%CI:0.150.99,p=0.05),the odds of being victimized by bullying was lower.Participants who adhered to both sleep duration and PA recommendations were less likely to present with severe behavioral problems(OR=0.17,95%CI:0.040.71,p<0.05)than those who did not meet those guidelines.Conclusion:Significant associations were found between adhering to 24-HMB guidelines and selected QoL indicators.These findings highlight the importance of maintaining a healthy lifestyle as a key factor in promoting and preserving the QoL of children with ASD. 展开更多
关键词 24-hour movement guidelines Autism spectrum disorder Physical activity Quality of life
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Look into my eyes:What can eye-based measures tell us about the relationship between physical activity and cognitive performance?
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作者 Liye Zou Fabian Herold +11 位作者 Sebastian Ludyga Keita Kamijo Notger G.Muller Matthew B.Pontifex Matthew Heath Ryuta Kuwamizu Hideaki Soya Charles H.Hillman Soichi Ando Brandon L.Alderman Boris Cheval Arthur F.Kramer 《Journal of Sport and Health Science》 SCIE CAS CSCD 2023年第5期568-591,共24页
Background:There is a growing interest to understand the neurobiological mechanisms that drive the positive associations of physical activity and fitness with measures of cognitive performance.To better understand tho... Background:There is a growing interest to understand the neurobiological mechanisms that drive the positive associations of physical activity and fitness with measures of cognitive performance.To better understand those mechanisms,several studies have employed eye-based measures(e.g., eye movement measures such as saccades,pupillary measures such as pupil dilation,and vascular measures such as retinal vessel diameter)deemed to be proxies for specific neurobiological mechanisms.However,there is currently no systematic review providing a comprehensive overview of these studies in the field of exercise-cognition science.Thus,this review aimed to address that gap in the literature.Methods:To identify eligible studies,we searched 5 electronic databases on October 23,2022.Two researchers independently extracted data and assessed the risk of bias using a modified version of the Tool for the assEssment of Study qualiTy and reporting in EXercise(TESTEX scale,for interventional studies) and the critical appraisal tool from the Joanna Briggs Institute(for cross-sectional studies).Results:Our systematic review(n=35 studies) offers the following main findings:(a) there is insufficient evidence available to draw solid conclusions concerning gaze-fixation-based measures;(b) the evidence that pupillometric measures,which are a proxy for the noradrenergic system,can explain the positive effect of acute exercise and cardiorespiratory fitness on cognitive performance is mixed;(c) physical training-or fitness-related changes of the cerebrovascular system(operationalized via changes in retinal vasculature) are,in general,positively associated with cognitive performance improvements;(d) acute and chronic physical exercises show a positive effect based on an oculomotor-based measure function(operationalized via antisaccade tasks);and(e) the positive association between cardiorespiratory fitness and cognitive performance is partly mediated by the dopaminergic system(operationalized via spontaneous eye-blink rate).Conclusion:This systematic review offers confirmation that eye-based measures can provide valuable insigt into the neurobiological mechanisms that may drive positive associations between physical activity and fitness and measures of cognitive performance.However,due to the limited number of studies utilizing specific methods for obtaining eye-based measures(e.g.,pupillometry,retinal vessel analysis,spontaneous eye blink rate) or investigating a possible dose-response relationship,further research is necessary before more nuanced conclusions can be drawn.Given that eye-based measures are economical and non-invasive,we hope this review will foster the future application of eye-based measures in the field of exercise-cognition science. 展开更多
关键词 COGNITION EXERCISE FITNESS Pupil size RetinaTagedAPTARAEnd
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ZnO材料的电子输运特性 被引量:3
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作者 郭宝增 王永青 +4 位作者 宗晓萍 孙荣霞 宋登元 Umberto Ravaioli Maritin Staedele 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2003年第7期723-728,共6页
用全带 Monte Carlo方法模拟了纤锌矿 Zn O材料电子的稳态和瞬态输运特性 .稳态输运特性包括稳态平均漂移速度 -电场特性、电子平均能量 -电场特性和在不同电场下电子按能量的分布 .在平均漂移速度 -电场特性中发现了微分负阻效应 .Zn ... 用全带 Monte Carlo方法模拟了纤锌矿 Zn O材料电子的稳态和瞬态输运特性 .稳态输运特性包括稳态平均漂移速度 -电场特性、电子平均能量 -电场特性和在不同电场下电子按能量的分布 .在平均漂移速度 -电场特性中发现了微分负阻效应 .Zn O的瞬态输运特性包括平均漂移速度 -位移关系曲线、渡越时间 -位移关系曲线等 .在平均漂移速度 -位移关系曲线中发现了过冲现象 ,这种现象是电子从低能谷到高能谷跃迁过程中的弛豫时间产生的 . 展开更多
关键词 MONTE Carlo ZNO 输运特性 微分负阻效应
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新风胶囊治疗对强直性脊柱炎患者BTLA^+T细胞数量和氧化应激的影响 被引量:21
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作者 齐亚军 刘健 +2 位作者 郑力 曹云祥 万磊 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2014年第10期1084-1089,共6页
目的探讨强直性脊柱炎(AS)患者B、T淋巴细胞衰减因子(BTLA)及活性氧(ROS)、活性氮(RNS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、总抗氧化能力(TAOC)的变化及新风胶囊(XFC)对其影响。方法将140例AS患者随机等分为XFC组(3... 目的探讨强直性脊柱炎(AS)患者B、T淋巴细胞衰减因子(BTLA)及活性氧(ROS)、活性氮(RNS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、总抗氧化能力(TAOC)的变化及新风胶囊(XFC)对其影响。方法将140例AS患者随机等分为XFC组(3粒/次,tid)和柳氮磺吡啶(SASP)组(4片/次,bid);连续治疗3个月。60例健康体检者为对照组。采用流式细胞术检测患者外周血BTLA表达频率及活化水平;采用ELISA检测2组血清中氧化应激指标(ROS、RNS、MDA、SOD、CAT、TAOC)和白细胞介素4(IL-4)、IL-10、IL-1β、肿瘤坏死因子α(TNF-α)含量;采用魏氏法检测血沉(ESR);采用日立7060型全自动生化分析仪检测高敏C反应蛋白(Hs-CRP)。结果 XFC组临床疗效显著优于SASP组,具有统计学意义(P<0.01)。与健康对照组相比,AS患者外周血CD3+T细胞、CD4+T细胞BTLA的水平显著降低(P<0.01或P<0.05);与对照组相比,AS患者SOD、CAT、TAOC值均显著降低,ROS、RNS、MDA值显著升高(P<0.01或P<0.05);与对照组相比,AS患者血清IL-1β、TNF-α和ESR、Hs-CRP值显著升高(P<0.01),IL-4、IL-10水平显著降低(P<0.01或P<0.05)。与治疗前相比,2组治疗后外周血BTLA+CD3+T细胞、BTLA+CD4+T细胞、SOD、TAOC、IL-4,SF-36量表生理机能(PF)、社会功能(SF)、生理职能(RP)、情感职能(RE)、躯体疼痛(BP)、精神健康(MH)、活力(VT)以及一般健康状况(GH)八个维度积分均显著升高,ROS、MDA、TNF-α、ESR、Hs-CRP、VAS、BASDAI、BASFI、BAS-G均显著降低(P<0.01或P<0.05)。XFC组与SASP组相比,差异有统计学意义(P<0.01或P<0.05)。Pearson相关分析结果显示:外周血BTLA表达水平与SOD、RP、BP、SF、RE呈正相关;BTLA+CD3+T细胞、BTLA+CD4+T细胞与ROS、MDA、IL-1β、TNF-α、ESR、VAS、BASDAI呈明显负相关,与TAOC、IL-4、IL-10呈正相关;BTLA+CD3+T细胞与RNS、Hs-CRP、BASFI呈明显负相关;BTLA+CD4+T细胞与CAT呈正相关。结论 XFC能增强AS患者外周血BTLA表达,负性调节T细胞的激活与增殖。 展开更多
关键词 强直性脊柱炎 B、T淋巴细胞衰减因子 氧化应激 新风胶囊
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基于Keap1-Nrf2-ARE探讨强直性脊柱炎患者心功能降低的机制 被引量:14
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作者 齐亚军 刘健 +4 位作者 郑力 万磊 曹云祥 孙玥 王芳 《中国免疫学杂志》 CAS CSCD 北大核心 2014年第5期654-661,共8页
目的:基于Keap1-Nrf2-ARE通路探讨强直性脊柱炎(AS)患者心功能降低的机制。方法:随机选取140例AS患者作为研究组,并抽取60例健康人作为正常对照组;采用多普勒超声心动图(UCG)检测两组心功能参数[E峰、A峰、E/A比值、左心房舒张期内径(LA... 目的:基于Keap1-Nrf2-ARE通路探讨强直性脊柱炎(AS)患者心功能降低的机制。方法:随机选取140例AS患者作为研究组,并抽取60例健康人作为正常对照组;采用多普勒超声心动图(UCG)检测两组心功能参数[E峰、A峰、E/A比值、左心房舒张期内径(LADd)、左心室舒张末期内径(LVDd)、左心室短轴缩短率(FS)、左心室射血分数(EF)、每搏输出量(SV)];采用ELISA法检测两组血清中通路蛋白[Keap1样ECH相关蛋白l(Keap1)、核因子NF-E2相关因子(Nrf2)]、氧化应激指标[丙二醛(MDA)、活性氧(ROS)、活性氮(RNS)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、总抗氧化能力(TAOC)]和细胞因子(IL-1β、TNF-α、IL-4、IL-10)含量;采用魏氏法检测炎性指标血沉(ESR);采用日立7060型全自动生化分析仪检测C反应蛋白(CRP)、免疫球蛋白。结果:(1)与正常对照组相比,AS活动期心功能参数(E峰、EF、E/A值)、抗氧化指标(SOD、CAT、TAOC)、细胞因子(IL-4、IL-10)值均显著降低(P<0.01或P<0.05);心功能参数A峰、通路蛋白(Keap1、Nrf2值)、氧化指标(ROS、RNS、MDA)、细胞因子(IL-1β、TNF-α)、炎性指标(ESR、CRP)值显著升高(P<0.01或P<0.05)。(2)与正常对照组相比,AS稳定期心功能参数[E峰、E/A值]、抗氧化指标(SOD、CAT、TAOC)、细胞因子(IL-4、IL-10)值均显著降低(P<0.01或P<0.05);心功能参数A峰、氧化指标(ROS、RNS、MDA)、细胞因子(TNF-α)、炎性指标(ESR、CRP)值显著升高(P<0.01或P<0.05)。(3)与AS稳定期相比,AS活动期心功能参数(E峰、EF、E/A值)、抗氧化指标(SOD、TAOC)、细胞因子(IL-10)值均显著降低(P<0.01或P<0.05);通路蛋白(Keap1、Nrf2)、氧化指标(ROS、RNS、MDA)、细胞因子(IL-1β、TNF-α)和炎性指标(ESR、CRP)、AS临床评价指标(VAS、BASDAI、BASFI、BASMI、BAS-G)值显著升高(P<0.01或P<0.05)。(4)与AS患者Keap1、Nrf2正常组比较,Keap1、Nrf2异常组心功能参数A峰显著升高,E峰、E/A值显著降低(P<0.05或P<0.01)。(5)相关性分析显示,心功能参数E峰与抗氧化指标(SOD、CAT、TAOC)、细胞因子(IL-4、IL-10)呈正相关,与通路蛋白(Keap1、Nrf2)、氧化指标(ROS、RNS、MDA)、细胞因子(IL-1β、TNF-α)、炎性指标(ESR、CRP)、AS临床评价指标(VAS、BASDAI、BASFI、BASMI、BAS-G)、免疫球蛋白G(IgG)、病程呈负相关;心功能参数A峰与通路蛋白(Keap1、Nrf2)、氧化指标(ROS、RNS、MDA)、细胞因子(IL-1β、TNF-α)、AS临床评价指标、心悸症状呈正相关,与通路蛋白Keap1、抗氧化指标(SOD、CAT、TAOC)、细胞因子(IL-4、IL-10)呈负相关;心功能参数E/A比值与抗氧化指标(SOD、CAT、TAOC)、细胞因子(IL-4、IL-10)呈正相关,与氧化指标(ROS、RNS、MDA)、细胞因子(TNF-α)、AS临床评价指标、病程呈负相关;心功能参数EF与病程呈负相关;心功能参数FS与通路蛋白Keap1呈负相关(P<0.05)。结论:33.56%的AS患者存在心功能降低,表现为心功能参数E峰、E/A值显著降低,A峰显著升高;心功能参数E峰、E/A值均与抗氧化指标、抑炎细胞因子呈正相关,与通路蛋白Keap1、Nrf2、氧化指标、致炎细胞因子、炎症指标呈负相关;而心功能参数A峰与通路蛋白Keap1、Nrf2、氧化指标、致炎细胞因子呈正相关,与抗氧化指标、抑炎细胞因子呈负相关。Keap1、Nrf2表达上升,Keap1-Nrf2-ARE信号通路活化后,机体抗氧化能力下降,促使细胞因子失衡、炎症指标升高,导致免疫复合物异常沉积增多,在引起AS发病的同时出现心功能降低。 展开更多
关键词 强直性脊柱炎 心功能 KEAP1 NRF2 氧化应激
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毒杀芬对孤儿受体雌激素相关受体1(ERRα-1)的拮抗作用 被引量:3
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作者 杨春 陈彬 +5 位作者 陈敏 陈健 李渝萍 周长保 周度金 陈煊 《第三军医大学学报》 CAS CSCD 北大核心 2001年第10期1183-1185,共3页
目的 研究毒杀芬 (Toxaphene)对孤儿受体雌激素相关受体 1 (Estrogen relatedreceptor,ERRα 1 )活性的影响。方法 细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。结果 毒杀芬抑制了由ERRα 1所介导的氯霉素酰基转移... 目的 研究毒杀芬 (Toxaphene)对孤儿受体雌激素相关受体 1 (Estrogen relatedreceptor,ERRα 1 )活性的影响。方法 细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。结果 毒杀芬抑制了由ERRα 1所介导的氯霉素酰基转移酶的转录表达和辅活化子 (Coactivator)GRIP1诱导的ERRα 1的活性。在构建的稳定高表达ERRα 1的HepG2肝癌细胞株中 ,ERRα 1增加了芳香族化酶的转录表达 ,ERRα 1高表达细胞株与 1 0 μmol/L浓度的毒杀芬温育 2 4h后 ,芳香族化酶活性降低到非ERRα 1转染的HepG2细胞中芳香族化酶活性的水平。结论 毒杀芬是ERRα 1的拮抗剂 ,它拮抗了ERRα 1调节芳香族化酶转录的作用 ,从而使该酶的表达量降低。 展开更多
关键词 雌激素相关受体 毒杀芬 孤儿受体 芳香族化酶
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代谢组学检测方法及其在中医药系统的应用 被引量:3
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作者 刘健 万磊 +10 位作者 黄传兵 汪元 曹云祥 刘磊 程园园 冯云霞 孙玥 齐亚军 王芳 张晓军 郑力 《中国临床保健杂志》 CAS 2013年第5期449-452,共4页
代谢组学是对生物体内代谢物进行定量分析,寻找代谢物与机体生理、病理变化关系的研究模式,是系统生物学的主要组成部分,它能更好地了解生物体中各种复杂体系的相互作用及生物系统对外界的响应,代谢组学的检测技术有核磁共振、气相色谱... 代谢组学是对生物体内代谢物进行定量分析,寻找代谢物与机体生理、病理变化关系的研究模式,是系统生物学的主要组成部分,它能更好地了解生物体中各种复杂体系的相互作用及生物系统对外界的响应,代谢组学的检测技术有核磁共振、气相色谱、高效液相色谱、气质联用、液质联用等。中医药研究在长期实践中积累了深厚的科学内涵,将代谢组学检测技术与中医药研究有机结合,使得代谢组学在中医药研究中发挥巨大潜力,代谢组学能在继承和发扬中医药优势和特色的基础上,研究生物体代谢规律和疾病发病本质,并阐明中药在体内作用环节和药物作用机制。 展开更多
关键词 诊断技术和方法 医学 中国传统 色谱法 液相 气相色谱一质谱法 磁共振 代谢组学 中药药 理学
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基于Keap1-Nrf2-ARE探讨强直性脊柱炎患者肺功能降低的机制 被引量:11
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作者 刘健 齐亚军 +4 位作者 郑力 万磊 曹云祥 王芳 《风湿病与关节炎》 2014年第7期9-16,共8页
目的:基于Keap1-Nrf2-ARE通路探讨强直性脊柱炎患者肺功能降低的机制。方法:选取120例强直性脊柱炎患者作为研究组,并从体检中心抽取60例健康人作为正常对照组。采用德国Jager MasterScreen自动肺功能检测仪测定两组肺功能参数[用力肺活... 目的:基于Keap1-Nrf2-ARE通路探讨强直性脊柱炎患者肺功能降低的机制。方法:选取120例强直性脊柱炎患者作为研究组,并从体检中心抽取60例健康人作为正常对照组。采用德国Jager MasterScreen自动肺功能检测仪测定两组肺功能参数[用力肺活量(FVC)、第1秒用力呼气容积(FEV1)、最大通气量(MVV)、最大呼气流量(PEF)、25%肺活量位的最大呼气流量(FEF25)、50%肺活量位的最大呼气流量(FEF50)、75%肺活量位的最大呼气流量(FEF75)];采用酶联免疫分析法(ELISA法)检测两组血清中通路蛋白[Kelch样ECH相关蛋白l(Keap1)、核因子NF-E2相关因子(Nrf2)]、氧化应激指标[丙二醛(MDA)、活性氧(ROS)、活性氮(RNS)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、总抗氧化能力(TAOC)]和细胞因子[白细胞介素(IL)-18、肿瘤坏死因子(TNF)-α、IL-4、IL-35]含量;采用魏氏法检测炎性指标红细胞沉降率(ESR);采用日立7060型全自动生化分析仪检测C-反应蛋白(CRP)、免疫球蛋白。结果:与正常对照组比较:①强直性脊柱炎患者FEV1、MVV、PEF、FEF50、FEF75值均显著降低(P<0.01或P<0.05);与强直性脊柱炎患者Keap1、Nrf2正常组比较,Keap1、Nrf2异常组FEV1、MVV、PEF、FEF50、FEF75值显著降低(P<0.05或P<0.01)。②强直性脊柱炎患者血清中Keap1、Nrf2、ROS、RNS、MDA值显著升高(P<0.01或P<0.05),SOD、CAT、TAOC值均显著降低(P<0.01或P<0.05)。③强直性脊柱炎患者IL-18、TNF-α、ESR、CRP值显著升高(P<0.01),IL-4、IL-35值显著降低(P<0.01或P<0.05)。④Spearman相关性分析显示,FEV1、MVV、PEF、FEF50、FEF75与SOD、CAT、TAOC、IL-4、IL-35呈正相关,与Keap1、Nrf2、ROS、RNS、MDA、IL-18、TNF-α、ESR、CRP、胸闷、气短、呼吸困难呈负相关(P<0.01或P<0.05)。结论:58.33%的强直性脊柱炎患者存在肺功能降低,表现为肺功能参数FEV1、MVV、PEF、FEF50、FEF75值均显著降低,以上肺功能参数与抗氧化指标、抑炎细胞因子呈正相关,与通路蛋白(Keap1、Nrf2)、氧化指标、致炎细胞因子、炎症指标呈负相关。Keap1、Nrf2表达上升,Keap1-Nrf2-ARE信号通路活化后,机体抗氧化能力下降,促使细胞因子失衡、炎症指标升高,导致免疫复合物异常沉积增多,在引起强直性脊柱炎发病的同时使肺组织或器官受损或破坏,最终出现肺功能降低。 展开更多
关键词 脊柱炎 强直性 Keap1-Nrf2-ARE 肺功能 KEAP1 NRF2 氧化应激
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抗GD2单链抗体-IL-2融合蛋白基因的构建及表达 被引量:2
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作者 倪剑锋 纪剑飞 +3 位作者 吕安国 黄日波 韦宇拓 吴文芳 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2007年第1期170-175,共6页
将已经突变了稀有密码子的人IL-2(Ala125)基因,与GD2单链抗体基因通过over-lap-PCR法,构建成ScFv-IL-2融合基因,并在基因的C端引入his tag序列。ScFv-IL-2基因经测序正确后连接到表达载体pSE380上,然后导入大肠杆菌BL21中表达。表达蛋... 将已经突变了稀有密码子的人IL-2(Ala125)基因,与GD2单链抗体基因通过over-lap-PCR法,构建成ScFv-IL-2融合基因,并在基因的C端引入his tag序列。ScFv-IL-2基因经测序正确后连接到表达载体pSE380上,然后导入大肠杆菌BL21中表达。表达蛋白为包涵体,表达量占总蛋白的30%以上,经SDS-PAGE及Western blot鉴定表达蛋白的分子量为43 kD,与预期的相符。包涵体粗提后,经Ni2+亲和层析柱及Sephacryl S-200HR柱分离复性,纯度可达90%以上,ELISA试验结果证明该融合蛋白保存了与相应抗原的结合活性和IL-2的生物活性。 展开更多
关键词 神经节苷脂GD2 单链抗体 白细胞介素2 融合蛋白
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核受体及核受体辅活化子对芳香族化酶基因转录调控的研究 被引量:1
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作者 周度金 杨春 +6 位作者 周长保 陈彬 陈敏 陈健 李渝萍 张放鸣 陈煊 《第三军医大学学报》 CAS CSCD 北大核心 2001年第12期1421-1424,共4页
目的 研究核受体及核受体辅活化子对芳香族化酶基因转录的调控。方法 酵母单、双杂合筛选、蛋白质 蛋白质相互作用分析、DNA突变、报告基因转染功能分析、迁移率改变及足迹分析等技术。结果 ①鉴定了主导芳香族化酶基因在乳腺癌细胞... 目的 研究核受体及核受体辅活化子对芳香族化酶基因转录的调控。方法 酵母单、双杂合筛选、蛋白质 蛋白质相互作用分析、DNA突变、报告基因转染功能分析、迁移率改变及足迹分析等技术。结果 ①鉴定了主导芳香族化酶基因在乳腺癌细胞中表达的启动子I.3和启动子Ⅱ的确切位置以及对这两个启动子起调节作用的沉默子 (Silencer)S1和cAMP效应要素 (CREaro)等顺式作用元件。②分离鉴定了能与类固醇衍生因子 1 (SterodogenicFactor1 ,SF1 )相互作用并参与芳香族化酶基因转录调控的转录因子 ,其中近 5 0 %的克隆编码两种新的富含脯氨酸的核受体辅调节蛋白质 ,命名为PNRC(Proline richNuclearReceptorCoactivators)。功能分析显示PNRC通过与SF1或ERR1相互作用 ,进一步增强SF1或ERR1对芳香族化酶基因启动子 1 .3的转录激活作用。③缺失突变及定点突变分析证明含SH3结合模体的 2 3个氨基酸残基区域是PNRC分子与核受体的相互作用位点。结论 我们鉴定了主导芳香族化酶基因在乳腺癌细胞中表达的启动子及调控序列 ,克隆了与DNA调控序列结合的蛋白质和通过蛋白质相互作用参与芳香族化酶基因转录调控的转录因子———一种新型的核受体辅活化子PNRC。 展开更多
关键词 核受体 芳香族化酶 辅活化子 PNRC 基因表达调控 乳腺癌
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用二维全带组合Monte Carlo方法模拟纤锌矿相GaN静电感应晶体管(SIT)特性 被引量:1
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作者 郭宝增 孙荣霞 Umberto Ravaioli 《电子学报》 EI CAS CSCD 北大核心 2003年第8期1121-1124,共4页
报告了用二维全带组合MonteCarlo方法模拟纤锌矿相GaN静电感应晶体管 (SITs)交直流特性的结果 .SIT的栅极长度为 0 13μm ,源极和漏极之间距离为 0 5 μm .模拟得到了SIT的输出特性 ,跨导和特征频率特性 .模拟得到的跨导最大值为 14 0... 报告了用二维全带组合MonteCarlo方法模拟纤锌矿相GaN静电感应晶体管 (SITs)交直流特性的结果 .SIT的栅极长度为 0 13μm ,源极和漏极之间距离为 0 5 μm .模拟得到了SIT的输出特性 ,跨导和特征频率特性 .模拟得到的跨导最大值为 14 0ms/mm(Vgs=- 1.5V) ,器件特征频率最大值为 12 3GHz(Ids=3 15A/cm) . 展开更多
关键词 MONTECARLO模拟 氮化镓 静电感应晶体管 化合物半导体
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猪血管紧张肽的质谱特性 被引量:1
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作者 黄河清 林庆梅 +1 位作者 MIAO Hai Won-Suk KIM 《动物学杂志》 CAS CSCD 北大核心 2003年第5期34-40,共7页
选用基质辅助激光解吸离子化飞行时间质谱技术研究不同激光强度对猪血管紧张肽 (AngiotensinⅠ ,AnⅠ )解吸离子化的百分率和一级结构的稳定性。结果表明 :激光强度与AnⅠ解吸离子化百分率有关 ,AnⅠ多聚体解吸离子化所需的激光强度高... 选用基质辅助激光解吸离子化飞行时间质谱技术研究不同激光强度对猪血管紧张肽 (AngiotensinⅠ ,AnⅠ )解吸离子化的百分率和一级结构的稳定性。结果表明 :激光强度与AnⅠ解吸离子化百分率有关 ,AnⅠ多聚体解吸离子化所需的激光强度高于单体。AnⅠ与海兔酸性多肽 (Aplysiaacidicpeptide,AP)和海兔胰岛素Cβ(AplysiainsulinCβ,AICβ)混合后 ,采用延迟引出技术和激光强度 2 3 0 0IU分析混合样品 ,该激光强度不影响对AnⅠ和它的多聚体解吸离子化 ,但对AICβ 影响较大 ,相当于丢失了一分子OH-。相同的激光强度对不同结构的多肽多聚体产生不同的解吸离子化百分率 ,优化激光强度是准确分析多肽结构信息关键因素之一。 展开更多
关键词 猪血管紧张肽 激光强度 质谱 多肽与混合多肽 解吸离子化
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抗GD2/抗CD16单链双特异性抗体的构建及在大肠杆菌中的表达 被引量:1
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作者 倪剑锋 纪剑飞 +4 位作者 白向阳 吕安国 黄日波 韦宇拓 吴文芳 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2007年第3期659-663,共5页
利用重叠PCR(overlap-PCR)将抗GD2表面抗原GD2的单链抗体基因m-ScFv和抗CD16的单链抗体基因NM3E2融合在一起,得到新型的单链双特异性抗体基因n-m,双抗的联接肽序列为SerGly4Ser,在肽连的C端引入了6×his以利于纯化。该双特异性抗体... 利用重叠PCR(overlap-PCR)将抗GD2表面抗原GD2的单链抗体基因m-ScFv和抗CD16的单链抗体基因NM3E2融合在一起,得到新型的单链双特异性抗体基因n-m,双抗的联接肽序列为SerGly4Ser,在肽连的C端引入了6×his以利于纯化。该双特异性抗体基因的序列经测序验证后,连接表达载体pET-22b(+),转化大肠杆菌菌株BL21(DE3),诱导表达,凝胶成像系统扫描显示表达的外源融合蛋白约占菌体总蛋白的27%。表达蛋白分泌于胞质空间,经超声波破胞后收集上清,经Ni+亲和层析柱分离,纯度可达90%以上。经SDS-PAGE及Westernblotting鉴定表达蛋白的分子量为53KD,与预期的相符。 展开更多
关键词 单链抗体 单链双特异性抗体 表达
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基于能带结构的布里渊区非均匀四面体网格生成技术 被引量:1
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作者 陈勇 RAVAIOLI Umberto 《计算物理》 CSCD 北大核心 2006年第4期477-482,共6页
基于非局域赝势能带计算及四面体网格单元中能量满足线性关系,提出一种布里渊区非均匀四面体网格产生方法,在满足精度条件下能自动得出数目最少的四面体网格,使布里渊区积分计算的精度和效率大为提高.通过对硅、锗两种金刚石结构半导体... 基于非局域赝势能带计算及四面体网格单元中能量满足线性关系,提出一种布里渊区非均匀四面体网格产生方法,在满足精度条件下能自动得出数目最少的四面体网格,使布里渊区积分计算的精度和效率大为提高.通过对硅、锗两种金刚石结构半导体简约布里渊区所产生网格的比较,表明该方法可以根据能带结构的特点自动生成优化的非均匀网格.对现有的态密度四面体计算公式进行了补充完善,并根据生成的网格和完善后的公式计算了硅和锗导带第一、二能带的态密度. 展开更多
关键词 四面体网格 简约布里渊区 非局域赝势 布里渊区积分
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