Differences in the population of genetics of Mycobacterium tuberculosis between urban migrants and local residents in Beijing, China

Background Currently, migration has become one of the risk factors of high burden of tuberculosis in China. This study was to explore the influence of mass migration on the dynamics of Mycobacterium （M.） tuberculosis in Beijing, the capital and an urban area of China.

Clinical analysis of adverse reactions in patients with multidrug-resistant and rifampicin-resistant pulmonary tuberculosis treated with delamanid-containing regimen

Objective To explore the characteristics of adverse drug reactions during the 24-week therapy with delamanid-containing regimen for patients with multidrug-resistant and rifampicin-resistant pulmonary tuberculosis(MDR/RR-PTB).

Evaluation of the efficacy of urine-based lipoarabinomannan antigen test in the diagnosis of pulmonary tuberculosis

Objective To analyze the diagnostic efficacy of urinary lipoarabinomannan (LAM) antigen detection method in tuberculosis patients,and to provide an experimental basis for the clinical application of urinary LAM kit in China.Methods From March to May 2023,228 patients with lung diseases [134 male,94 female,age 20-82 (44.8±16.7) years] were prospectively collected in Beijing Chest Hospital,Capital Medical University,including 143 pulmonary tuberculosis patients and 85 non-tuberculosis patients.

Evaluation of T-SPOT.TB assay in the diagnosis of pulmonary tuberculosis within different age groups

Objective To evaluate the value of T-SPOT.TB assay in the diagnosis of pulmonary tuberculosis within different age groups.Methods We analyzed 1 518 suspected pulmonary tuberculosis(PTB)patients who were admitted to the Beijing Chest Hospital from November 2012to February 2014 and had valid T-SPOT.TB tests

CT imaging of coexisting pulmonary tuberculosis and lung cancer

Objective To study the CT characteristics of coexisting pulmonary tuberculosis and lung cancer. Methods One hundred and four patients of coexisting pulmonary tuberculosis and lung cancer proved by histology,cytology or clinical underwent CT examination. All patients were divided into two groups。

Early secretory antigenic target protein-6/culture filtrate protein-10 fusion protein-specific Th1 and Th2 response and its diagnostic value in tuberculous pleural effusion

Objective To detect the Th1 and Th2 cell percentage in pleural effusion mononuclear cells (PEMCs) stimulated by early secretory antigenic target protein-6 (ESAT-6) /culture filtrate protein-10 (CFP-10) fusion protein (E/C) with flow cytometry (FCM) ,and to explore the local antigen specific Th1 and Th2 response

Comparison of interferon-gamma release assays and adenosine deaminase of pleural fluid for the diagnosis of pleural tuberculosis

Objective To compare the diagnostic performance of interferon gamma releasing assays(T-SPOT.TB)and adenosine deaminase(ADA)in pleural tuberculosis,and therefore to evaluate the value of T-SPOT.TB in a high tuberculosis burden country.Methods From June 2011to November 2012,111 patients with pleural fluid in Beijing Chest Hospital,Capital Medical University

Treatment effect analysis of the standard regimen and the optimized regimen for retreatment pulmonary tuberculosis complicated with diabetes

Objective To analyze the therapeutic effects of the standard regimen and the optimized regimen in retreatment pulmonary tuberculosis complicated with diabetesmellitus(DM).Methods In a multi-center cohort study,patients with smear positive retreatment pulmonary tuberculosis(TB)with DM and those without

Clinical analysis of protionamide and para-aminosalicylic acid induced hepatotoxicity in 129 cases

Objective To investigate drug-induced liver injury (DILI) in tuberculosis (TB) patients treated with protionamide (Pto) and (or) para-aminosalicylic acid (PAS) ,and therefore to provide data for using secondline anti-tuberculosis drugs and risk prediction of liver damage.Methods A retrospective analysis was performed for TB patients treated with regimens

The value of cell free DNA of Mycobacterium tuberculosis in the early diagnosis of tuberculous meningitis

Objective To detect the cell-free DNA of Mycobacterium tuberculosis(Cf-TB)in the cerebrospinal fluid(CSF)of patients with tuberculous meningitis(TBM),and to assess the diagnostic value of this method for TBM.Methods We prospectively included patients with suspected meningitis from the Department of Tuberculosis,Beijing Chest Hospital,Department of Neurology,Beijing Chaoyang Hospital and Department of Neurology,263 Hospital of the People’s Liberation Army from September 2019 to March 2022.

Diagnostic performance of a novel Mycobacterium tuberculosis specific T-Cell based assay for tuberculosis

Objective To evaluate the diagnosic performance of a novel Mycobacterium tuberculosis(MTB)specific T-cell based assay for tuberculosis,which targets the mRNA detection of interferon gamma-induced protein 10(IP-10).Methods Suspected tuberculosis patients were prospectively and consecutively recruited in Beijing Chest Hospital between March 2018 and November 2019.

Detection of tuberculosis genes associated with drug-resistance in paraffin-embedded tissue specimens using next generation sequencing technology

Objective To evaluate the use of multiplex PCR amplicon sequencing(mPCR-NGS)technology in detecting gene mutations related to drug resistance of Mycobacterium tuberculosis(MTB)in formalin-fixed paraffin-embedded tissue specimens,and to explore its clinical value in the diagnosis of drug-resistant tuberculosis.Methods Fifty clinical MTB strains isolated in the Changping District Tuberculosis Control Institute of Beijing from April 2013 to October 2015 with drug susceptibility test(DST)results of rifampicin,isoniazid,ethambutol,streptomycin,ofloxacin,capreomycin,kanamycin and amikacin available were recovered,including 42 drug-resistant strains and 8 drug-sensitive strains.

In vitro evaluation of the antibacterial activity of nemonoxacin against Mycobacterium tuberculosis,Mycobacterium intracellulare and Mycobacterium abscessus

Objective To test the in vitro antibacterial activity of nemonoxacin against clinically isolates of Mycobacterium tuberculosis complex(MTBC),Mycobacterium intracellulare(MI)and Mycobacterium abscessus(MA).Methods Totally 128,80 and 50 isolates of MTBC,M.intracellulare and M.abscessus were tested,respectively.The minimum inhibitory concentrations(MICs)of nemonoxacin and levofloxacin against the strains of the three most frequently isolated mycobacterium species were measured by double dilution method with micro-well plate.

Effect of Mycobacterium tuberculosis protein Rv0309 on intracellular survival of Mycobacterium smegmatis by inhibiting macrophage autophagy via protein STUB1

Objective To investigate the molecular regulatory mechanism of Mycobacterium tuberculosis(MTB)protein Rv0309 to promote the survival of Mycobacterium smegmatis(Ms)in macrophages.Methods Using Ms as a model to study Mycobacterium tuberculosis,recombinant Ms transfected with pMV261 and PMV261-RV0309 in the control group and RA W264.7 cells were constructed.

Protective effect of anti-idiopathic pulmonary fibrosis drug Pirfenidone and Sufenidone(SC1011)on pulmonary injury induced by tuberculosis in a mouse tuberculosis model

Objective To evaluate the protective effect of antiidiopathic pulmonary fibrosis(IPF)marketed drug Pirfenidone and its clinical drug Sufenidone(SC1011)against lung injury in a mouse tuberculosis model.Methods C57BL/6 mouse model of tuberculosis was established.A total of 75 C57BL/6 mice were infected with 1×10^(7) CFU/ml H37Rv suspension by aerosol and randomly divided into untreated(n=9)group.