Cheng Zai Wan (承载丸), a Chinese herbal preparation was administrated in the two-leg rat model of asepticnecrosis of the femoral head established by taking prednisone acetate for a long period and the osteoporosismod...Cheng Zai Wan (承载丸), a Chinese herbal preparation was administrated in the two-leg rat model of asepticnecrosis of the femoral head established by taking prednisone acetate for a long period and the osteoporosismodel rat by castration in order to explore the effects of the prescription on necrosis of the femoral head. Theresults showed that after treatment, the pitting on the surface of the femoral head disappeared, the reticularstructure with filling cells was restored; the fat droplets in bone cells or cartilage cells of the femoral headwere significantly reduced; sparse capillaries were improved, density and width of the bone trabecula wereincreased somewhat; bone mineral density, bone weight, bone strength and rigidity were significantlyincreased; and the low level of estrin was improved. It is suggested that Cheng Zai Wan has definitetherapeutic effects on aseptic necrosis of the femoral head.展开更多
Objective: To observe the effect of the serum containing Chengzai Pill on the L-type voltage-sensitive calcium channels current (L-VSCCsC) of osteoblastic MC3T3-E1 cells pretreated with methylprednisolone (mPSL). Meth...Objective: To observe the effect of the serum containing Chengzai Pill on the L-type voltage-sensitive calcium channels current (L-VSCCsC) of osteoblastic MC3T3-E1 cells pretreated with methylprednisolone (mPSL). Methods: A control group, a model group, a low dose group and a high dose group were set up. The whole cell patch clamp technique was used to record L-VSCCsC of 10 osteoblastic MC3T3-E1 cells in each group and their peak currents were determined. Results: The peak current of the control group was 0.2284±0.0209 nA; the peak current of the model group was 0.1839±0.0179 nA; decreased by 19.5% as compared with the control group (P<0.01); the peak current of the low and high dose groups was 0.2526± 0.0093 nA and 0.2671±0.0120 nA respectively, increased by 37.4% and 45.2% as compared with the model group (P<0.01); the difference between the low and high dose groups was P<0.05. Conclusion: 1. mPSL inhibits L-VSCCsC of osteoblasts; and 2. The serum containing Chengzai Pill increases L-VSCCsC of osteoblasts pretreated with mPSL.展开更多
文摘Cheng Zai Wan (承载丸), a Chinese herbal preparation was administrated in the two-leg rat model of asepticnecrosis of the femoral head established by taking prednisone acetate for a long period and the osteoporosismodel rat by castration in order to explore the effects of the prescription on necrosis of the femoral head. Theresults showed that after treatment, the pitting on the surface of the femoral head disappeared, the reticularstructure with filling cells was restored; the fat droplets in bone cells or cartilage cells of the femoral headwere significantly reduced; sparse capillaries were improved, density and width of the bone trabecula wereincreased somewhat; bone mineral density, bone weight, bone strength and rigidity were significantlyincreased; and the low level of estrin was improved. It is suggested that Cheng Zai Wan has definitetherapeutic effects on aseptic necrosis of the femoral head.
文摘Objective: To observe the effect of the serum containing Chengzai Pill on the L-type voltage-sensitive calcium channels current (L-VSCCsC) of osteoblastic MC3T3-E1 cells pretreated with methylprednisolone (mPSL). Methods: A control group, a model group, a low dose group and a high dose group were set up. The whole cell patch clamp technique was used to record L-VSCCsC of 10 osteoblastic MC3T3-E1 cells in each group and their peak currents were determined. Results: The peak current of the control group was 0.2284±0.0209 nA; the peak current of the model group was 0.1839±0.0179 nA; decreased by 19.5% as compared with the control group (P<0.01); the peak current of the low and high dose groups was 0.2526± 0.0093 nA and 0.2671±0.0120 nA respectively, increased by 37.4% and 45.2% as compared with the model group (P<0.01); the difference between the low and high dose groups was P<0.05. Conclusion: 1. mPSL inhibits L-VSCCsC of osteoblasts; and 2. The serum containing Chengzai Pill increases L-VSCCsC of osteoblasts pretreated with mPSL.
