The technique in situ hybridization was used to measure the levels of light （NF-L）,medium（NF-M） and heavy （NF-H） neurofilament protein subunits mRNA in L<sub>4-6</sub> spinal motoneurons in adult rat...The technique in situ hybridization was used to measure the levels of light （NF-L）,medium（NF-M） and heavy （NF-H） neurofilament protein subunits mRNA in L<sub>4-6</sub> spinal motoneurons in adult rat during regeneration following a unilateral crush of the sciatic nerve.It was found that the hybridization signals of each neurofilament subunit mRNA were dramatically decreased in spinal motoneurons postaxotomy by light microscopy. The hybridization signals of NF-L and NF-M mRNA were located in cytoplasm of neurons, whereas NF-H mRNA was found in both nucleus and cytoplasm of neurons. lmage analysis showed that the encoding levels of mRNA for each of neurofilament subunit mRNA reduced on the 3rd d and returned to control levels on the 28th d following the lesion. The relative levels of mRNA coding for each neurofilament subunit were significantly different. The lowest level of NF-L mRNA was observed at 5 d postaxotomy, and that of NF-M, NF-H mRNA on the 7th and 10th d after injury. Moreover,the levels of HF-M and展开更多
文摘The technique in situ hybridization was used to measure the levels of light （NF-L）,medium（NF-M） and heavy （NF-H） neurofilament protein subunits mRNA in L<sub>4-6</sub> spinal motoneurons in adult rat during regeneration following a unilateral crush of the sciatic nerve.It was found that the hybridization signals of each neurofilament subunit mRNA were dramatically decreased in spinal motoneurons postaxotomy by light microscopy. The hybridization signals of NF-L and NF-M mRNA were located in cytoplasm of neurons, whereas NF-H mRNA was found in both nucleus and cytoplasm of neurons. lmage analysis showed that the encoding levels of mRNA for each of neurofilament subunit mRNA reduced on the 3rd d and returned to control levels on the 28th d following the lesion. The relative levels of mRNA coding for each neurofilament subunit were significantly different. The lowest level of NF-L mRNA was observed at 5 d postaxotomy, and that of NF-M, NF-H mRNA on the 7th and 10th d after injury. Moreover,the levels of HF-M and
