BACKGROUND: Early diagnosis of liver metastasis of colorectal carcinoma is very important for the appropriate treatment of such patients. However, there has been no effective approach available for clinical applicatio...BACKGROUND: Early diagnosis of liver metastasis of colorectal carcinoma is very important for the appropriate treatment of such patients. However, there has been no effective approach available for clinical application. The present study aimed to investigate the differential expression of proteins in patients with liver metastasis of colorectal carcinomas using proteomic analysis and evaluate its potentiality in clinical diagnosis. METHODS: Fluorescence two-dimensional differential in-gel electrophoresis (2-D DIGE) was used to analyze and compare the protein expression between normal mucosa, the primary focus, and liver metastases. Proteomic analysis was made to identify the differentially expressed proteins. Immunohistological staining was used to confirm the expression of differentially expressed proteins in colorectal carcinomas and areas of liver metastasis. RESULTS: A 1.5-fold difference was found with 46 differentially expressed proteins. In 20 differentially expressed proteins, 3 were down-regulated and 17 up-regulated in liver metastases. Proteomic analysis showed that the S-adenosylmethionine transgelin variant was down-regulated in liver metastasis tissues. Zinc finger protein 64 homolog (Zfp64), guanine nucleotide exchange factor 4 (GEF4), human arginase, glutathione S-transferases (GSTs) A3, and tumor necrosis factor a (TNF-alpha)-induced protein 9 were up-regulated in liver metastasis tissues. Immunohistochemical staining confirmed that human arginase expression was higher in liver metastases than in the primary focus. CONCLUSIONS: There was a significant difference in protein expression between the primary focus of colorectal carcinoma and liver metastases. The differentially regulated proteins were closely related to liver metastasis of colorectal carcinoma. Elevated human arginase may be an important molecular marker for liver metastasis from colorectal carcinoma. (Hepatobiliary Pancreat Dis Jut 2010; 9: 149-153)展开更多
To the editor： Invasive fungal infections （IFIs） are significant complications in liver transplant recipients, which are associated with high morbidity/mortality and higher healthcare costs. The incidence of IFIs i...To the editor： Invasive fungal infections （IFIs） are significant complications in liver transplant recipients, which are associated with high morbidity/mortality and higher healthcare costs. The incidence of IFIs is mainly influenced by the patients＇ clinical condition, the level of immune suppression, surgical factors and the technical complexity of the surgery. We read with great interest the research article by Shi et alJ Although they have reached a high curative ratio with their ＂experiential＂ therapy based on their previous clinical experience, they did not provide us with detailed, definite criteria for identifying suspected patients and allowing for their early ＂experiential＂ treatment. Updated, standardized guidelines from the Invasive Fungal Infections展开更多
To tile editor： Systemic lupus erythenlatosus （SLE） can have multiple neuropsychiatric manifestations, which also are main reasons of disability and death. Neuromyelitis optica （Devic＇s syndrome/NMO） is one of t...To tile editor： Systemic lupus erythenlatosus （SLE） can have multiple neuropsychiatric manifestations, which also are main reasons of disability and death. Neuromyelitis optica （Devic＇s syndrome/NMO） is one of the rarest and most serious neurological manifestations, with limited cases reportedJ Here, we report a patient who developed neuromyelitis optica with SLE.展开更多
基金supported by grants from the National Natural Science Foundation of China(No.30471700)the "Tenth one five"Science and Technique Foundation of the PLA,China(No.06G027)
文摘BACKGROUND: Early diagnosis of liver metastasis of colorectal carcinoma is very important for the appropriate treatment of such patients. However, there has been no effective approach available for clinical application. The present study aimed to investigate the differential expression of proteins in patients with liver metastasis of colorectal carcinomas using proteomic analysis and evaluate its potentiality in clinical diagnosis. METHODS: Fluorescence two-dimensional differential in-gel electrophoresis (2-D DIGE) was used to analyze and compare the protein expression between normal mucosa, the primary focus, and liver metastases. Proteomic analysis was made to identify the differentially expressed proteins. Immunohistological staining was used to confirm the expression of differentially expressed proteins in colorectal carcinomas and areas of liver metastasis. RESULTS: A 1.5-fold difference was found with 46 differentially expressed proteins. In 20 differentially expressed proteins, 3 were down-regulated and 17 up-regulated in liver metastases. Proteomic analysis showed that the S-adenosylmethionine transgelin variant was down-regulated in liver metastasis tissues. Zinc finger protein 64 homolog (Zfp64), guanine nucleotide exchange factor 4 (GEF4), human arginase, glutathione S-transferases (GSTs) A3, and tumor necrosis factor a (TNF-alpha)-induced protein 9 were up-regulated in liver metastasis tissues. Immunohistochemical staining confirmed that human arginase expression was higher in liver metastases than in the primary focus. CONCLUSIONS: There was a significant difference in protein expression between the primary focus of colorectal carcinoma and liver metastases. The differentially regulated proteins were closely related to liver metastasis of colorectal carcinoma. Elevated human arginase may be an important molecular marker for liver metastasis from colorectal carcinoma. (Hepatobiliary Pancreat Dis Jut 2010; 9: 149-153)
文摘To the editor： Invasive fungal infections （IFIs） are significant complications in liver transplant recipients, which are associated with high morbidity/mortality and higher healthcare costs. The incidence of IFIs is mainly influenced by the patients＇ clinical condition, the level of immune suppression, surgical factors and the technical complexity of the surgery. We read with great interest the research article by Shi et alJ Although they have reached a high curative ratio with their ＂experiential＂ therapy based on their previous clinical experience, they did not provide us with detailed, definite criteria for identifying suspected patients and allowing for their early ＂experiential＂ treatment. Updated, standardized guidelines from the Invasive Fungal Infections
文摘To tile editor： Systemic lupus erythenlatosus （SLE） can have multiple neuropsychiatric manifestations, which also are main reasons of disability and death. Neuromyelitis optica （Devic＇s syndrome/NMO） is one of the rarest and most serious neurological manifestations, with limited cases reportedJ Here, we report a patient who developed neuromyelitis optica with SLE.
