A new alpha-pyrone derivative, epimedokoreanone A was isolated from the aerial parts of Epimedium koreanum Nakai. Its structure was determined by spectroscopic methods (IR, MS, H-1 NMR, C-13 NMR, DEPT, H-1-C-13 COSY a...A new alpha-pyrone derivative, epimedokoreanone A was isolated from the aerial parts of Epimedium koreanum Nakai. Its structure was determined by spectroscopic methods (IR, MS, H-1 NMR, C-13 NMR, DEPT, H-1-C-13 COSY and H-1-C-13 long range COSY)展开更多
BACKGROUND: The differential diagnosis of solid lesions located at the pancreatic head is very important for choosing therapies and setting up surgical tactics. This study was designed to evaluate the clinical signifi...BACKGROUND: The differential diagnosis of solid lesions located at the pancreatic head is very important for choosing therapies and setting up surgical tactics. This study was designed to evaluate the clinical significance of combined measurement of multiple serum tumor markers and the application of the receiver-operating characteristic (ROC) curves in the differential diagnosis of solid lesions located at the pancreatic head. METHODS: The serum levels of CA19-9, CA242, CA50 and carcinoembryonic antigen (CEA) in 112 patients with carcinoma of the pancreatic head and 38 patients with focal chronic pancreatitis in the pancreatic head were measured with ELISA. The sensitivity, specificity, positive likelihood ratio (PLR) and negative likelihood ratio (NLR) of the four serum tumor markers were calculated. The ROC curves for the four serum tumor markers were constructed and the area under the curve (AUC) was calculated. RESULTS: The AUCs of CA19-9, CA242, CA50 and CEA were 0.805, 0.749, 0.738 and 0.705; the PLRs were 1.91, 3.43, 5.09 and 5.46; and the NLRs were 0.41, 0.56, 0.59 and 0.71, respectively. Combined measurements increased the diagnostic specificity, and parallel combined testing increased the diagnostic sensitivity. CONCLUSIONS: Combined measurement of serum tumor markers CA19-9, CA242, CA50 and CEA is valuable in differential diagnosis of solid lesions located at the pancreatic head, and CA19-9 has the best diagnostic ability. Combined measurements can increase the specificity of diagnosis. Evaluation with the ROC curve is better than the sensitivity or specificity alone and the results are more integrated and objective.展开更多
Two new glycosides 1,5-dihydroxyxathanone-6-o-beta-D-glucoside and flavanonol-taxifolin-3, 7-o-alpha-L-dirhamnoside. were isolated from the aerial parts of Hypericum japonicum Thunb, ex Murray, The chemical structures...Two new glycosides 1,5-dihydroxyxathanone-6-o-beta-D-glucoside and flavanonol-taxifolin-3, 7-o-alpha-L-dirhamnoside. were isolated from the aerial parts of Hypericum japonicum Thunb, ex Murray, The chemical structures were determined by spectroscopic methods and chemical evidences展开更多
Abstract: Two new depsidic glycosides salviaflasied and its methyl ester were isolated from the aqueous extract of Salvaia flava. their structures were elucidated by spectral analysis, especially by 2D NMR analysis.
A new Annonaceous acetogenin, named uvarigranin(I),was isolated from the ethanol extract of the roots of Uvaria grandiflora Roxb, together with the known isodesacetyluvaricin (II) Their plane structures and relative c...A new Annonaceous acetogenin, named uvarigranin(I),was isolated from the ethanol extract of the roots of Uvaria grandiflora Roxb, together with the known isodesacetyluvaricin (II) Their plane structures and relative configurations were established on the basis of NMR and MS spectroscopic methods. Compound I belongs to a mono(-1)-trahydrofuran (THF) type acetogenin with an unusual acetoxy group at the adjacent carbon on the THF ring.展开更多
Subvellerolactone C(I), a new 3, 8-oxa-13-hydroxy-1 acrar-6-en-5-oic acid γ-lactone was isolated from the fruit bodies of Lactarius subvellereus (Russulaceae), and its structure was elucidated by means of spectroscop...Subvellerolactone C(I), a new 3, 8-oxa-13-hydroxy-1 acrar-6-en-5-oic acid γ-lactone was isolated from the fruit bodies of Lactarius subvellereus (Russulaceae), and its structure was elucidated by means of spectroscopic methods(UV, IR, HRMS,1H-NMR, 13C-NMR, 13c 1HCORR., 1H- 1HCOSY, HMBC, NOEDIFF.).展开更多
A series of subshtuted 3'-N-benzoyl taxol analogs [7a￣i] were synthesized by Schotten-Baumann acylation of the key intermediate, 13-O-[(2'R, 3' S) -3'-phenylisoserinoyl]baccatin Ⅲ[6]. Evaluation of t...A series of subshtuted 3'-N-benzoyl taxol analogs [7a￣i] were synthesized by Schotten-Baumann acylation of the key intermediate, 13-O-[(2'R, 3' S) -3'-phenylisoserinoyl]baccatin Ⅲ[6]. Evaluation of the newly ￣ derivatives for cytotoxicity against KB Cell line revealed that electrondon-donating groups at the para position (such as -CH3, -OCH3, etc.) havepositive effect on achvity, while electron-attracting groups(such as -NO2, -Br, etc.) have negativeeffect. Sterically hindered substituents at the ortho position could dramatically reduce the activity.But none of the analogs had higher cytotoxicity than taxol.展开更多
A new bisindole alkaloid, melaxillarinine, was isolated from the roots of Melodinus axillaris. Its structure was established on the basis of spectral analysis.
A new stilbene diglucoside, gnetifolin K, was isolated from the lianas of Gnetum parvifolium. Its structure was established as isorhapotigenin-3,4'-di-O-beta-D-glucoside on the basis of spectral and chemical evide...A new stilbene diglucoside, gnetifolin K, was isolated from the lianas of Gnetum parvifolium. Its structure was established as isorhapotigenin-3,4'-di-O-beta-D-glucoside on the basis of spectral and chemical evidence.展开更多
A new basic dodecapeptide BPP was isolated from the water-extract of the buckwheat pollen (BP). The sequence of BPP, which was first reported, was determined by DABITC/PITC double coupling method, and confirmed by che...A new basic dodecapeptide BPP was isolated from the water-extract of the buckwheat pollen (BP). The sequence of BPP, which was first reported, was determined by DABITC/PITC double coupling method, and confirmed by chemical synthesis. Some analogues of BPP were also synthesized by SPPS Method. The effect of these peptides on spleen lymphocytes from mice of Kunming inbred line and on release of SIL-2R from human lymphocytes were studied. The results showed that these peptides exhibit certain immune activity.展开更多
A d,I mixture (R-R and S-S) can be resolved into the d and the I enantiomers. while the mesoisomer (R-S, identical with S-R) cannot be physically separated. This fact has occasionally been 'extrapolated' to di...A d,I mixture (R-R and S-S) can be resolved into the d and the I enantiomers. while the mesoisomer (R-S, identical with S-R) cannot be physically separated. This fact has occasionally been 'extrapolated' to differentiate d,l and meso isomers with a chiral shift reagent where the meso isomer is presumed not to show any signal 'doubling' during the (usually) downfield shift. This assumption hasbeen shown to be incorrect by the behavior of a meso molecule which does give signal 'doubling'.展开更多
A high-performance liquid chromatographic method using uv detection for thedetermination of 10 drugs of abuse is described. The method is sample and sensitive. The linear rangesare 20￣100ng/ml with relative standard ...A high-performance liquid chromatographic method using uv detection for thedetermination of 10 drugs of abuse is described. The method is sample and sensitive. The linear rangesare 20￣100ng/ml with relative standard deviations of 0.7 ￣ 2.6% (n=7) and detection limits of 5￣ 10ng/ml.展开更多
Four new taxoids with rearranged taxane skeleton,named taxawallins C(1),D(2), F(3)and H(4),were isolated from the needles of Taxus wallichiana Zucc.. Their structures were elucidated by spectroscopic techniques.
From the aerial part of Hypericum japonicum Thunb. ex Murray, two new chromone glycosides, 5, 7-Dihydroxy-2-(1-methylpropyl) chromone-8-beta-D-glucoside and 5, 7-Dihydroxy-2-isopropylchromone-8-beta-D-glucoside, were ...From the aerial part of Hypericum japonicum Thunb. ex Murray, two new chromone glycosides, 5, 7-Dihydroxy-2-(1-methylpropyl) chromone-8-beta-D-glucoside and 5, 7-Dihydroxy-2-isopropylchromone-8-beta-D-glucoside, were isolated and their structures were determined on basis of spectroscopic evidences.展开更多
Howiinol A, an active antitumor constituent from the root and stem bark of Goniothamus howii Meer. (Annoaceae) has been synthesized in nine steps from alpha - D - glucoheptonic gamma - lactone with an overall yield of...Howiinol A, an active antitumor constituent from the root and stem bark of Goniothamus howii Meer. (Annoaceae) has been synthesized in nine steps from alpha - D - glucoheptonic gamma - lactone with an overall yield of 12.4%. The absolute configuration of natural howiinol A is further confirmed.展开更多
The absolute stereochemistries of Annonaceous acetogenins, tonkinin A and B, have been established by the use of the convenient conversion to their formaldehyde acetal derivatives and the subsequent application of the...The absolute stereochemistries of Annonaceous acetogenins, tonkinin A and B, have been established by the use of the convenient conversion to their formaldehyde acetal derivatives and the subsequent application of the Mosher ester methodology.展开更多
7-Epi-howiinol A, an antitumor lactone, has been synthesized in nine steps starting from alpha-D-glucoheptonic gamma-lactone with an overall yield of 8.5%.
A novel bioactive Annonaceous acetogenin, donhexocin (1), has been isolated from the roots of Goniothalamus donnaiensis (Annonaceae). 1 is a new acetogenin lacking THF rings but bearing six hydroxyls including two vic...A novel bioactive Annonaceous acetogenin, donhexocin (1), has been isolated from the roots of Goniothalamus donnaiensis (Annonaceae). 1 is a new acetogenin lacking THF rings but bearing six hydroxyls including two vic-diol systems in the aliphatic chain. Its structure and stereochemistry has been elucidated by spectoscopic analysis and derivatization.展开更多
A derivative of 2-arylbenzofuranoid pattern fashioned after the naturally occurring isolates from Salviamiltiorrhiza was synthesized by Perkin condensation and oxidative ring closure.
文摘A new alpha-pyrone derivative, epimedokoreanone A was isolated from the aerial parts of Epimedium koreanum Nakai. Its structure was determined by spectroscopic methods (IR, MS, H-1 NMR, C-13 NMR, DEPT, H-1-C-13 COSY and H-1-C-13 long range COSY)
基金This study was supported by a grant from Clinical Subject of Ministry of Health of China (2004-2006-2).
文摘BACKGROUND: The differential diagnosis of solid lesions located at the pancreatic head is very important for choosing therapies and setting up surgical tactics. This study was designed to evaluate the clinical significance of combined measurement of multiple serum tumor markers and the application of the receiver-operating characteristic (ROC) curves in the differential diagnosis of solid lesions located at the pancreatic head. METHODS: The serum levels of CA19-9, CA242, CA50 and carcinoembryonic antigen (CEA) in 112 patients with carcinoma of the pancreatic head and 38 patients with focal chronic pancreatitis in the pancreatic head were measured with ELISA. The sensitivity, specificity, positive likelihood ratio (PLR) and negative likelihood ratio (NLR) of the four serum tumor markers were calculated. The ROC curves for the four serum tumor markers were constructed and the area under the curve (AUC) was calculated. RESULTS: The AUCs of CA19-9, CA242, CA50 and CEA were 0.805, 0.749, 0.738 and 0.705; the PLRs were 1.91, 3.43, 5.09 and 5.46; and the NLRs were 0.41, 0.56, 0.59 and 0.71, respectively. Combined measurements increased the diagnostic specificity, and parallel combined testing increased the diagnostic sensitivity. CONCLUSIONS: Combined measurement of serum tumor markers CA19-9, CA242, CA50 and CEA is valuable in differential diagnosis of solid lesions located at the pancreatic head, and CA19-9 has the best diagnostic ability. Combined measurements can increase the specificity of diagnosis. Evaluation with the ROC curve is better than the sensitivity or specificity alone and the results are more integrated and objective.
文摘Two new glycosides 1,5-dihydroxyxathanone-6-o-beta-D-glucoside and flavanonol-taxifolin-3, 7-o-alpha-L-dirhamnoside. were isolated from the aerial parts of Hypericum japonicum Thunb, ex Murray, The chemical structures were determined by spectroscopic methods and chemical evidences
文摘Abstract: Two new depsidic glycosides salviaflasied and its methyl ester were isolated from the aqueous extract of Salvaia flava. their structures were elucidated by spectral analysis, especially by 2D NMR analysis.
文摘A new Annonaceous acetogenin, named uvarigranin(I),was isolated from the ethanol extract of the roots of Uvaria grandiflora Roxb, together with the known isodesacetyluvaricin (II) Their plane structures and relative configurations were established on the basis of NMR and MS spectroscopic methods. Compound I belongs to a mono(-1)-trahydrofuran (THF) type acetogenin with an unusual acetoxy group at the adjacent carbon on the THF ring.
文摘A new taxoid, taxuchin B[1], has been isolated from twigs and needles of Taxus chinensis. It is the first chlorine-containing taxoid reported to date.
文摘Subvellerolactone C(I), a new 3, 8-oxa-13-hydroxy-1 acrar-6-en-5-oic acid γ-lactone was isolated from the fruit bodies of Lactarius subvellereus (Russulaceae), and its structure was elucidated by means of spectroscopic methods(UV, IR, HRMS,1H-NMR, 13C-NMR, 13c 1HCORR., 1H- 1HCOSY, HMBC, NOEDIFF.).
文摘A series of subshtuted 3'-N-benzoyl taxol analogs [7a￣i] were synthesized by Schotten-Baumann acylation of the key intermediate, 13-O-[(2'R, 3' S) -3'-phenylisoserinoyl]baccatin Ⅲ[6]. Evaluation of the newly ￣ derivatives for cytotoxicity against KB Cell line revealed that electrondon-donating groups at the para position (such as -CH3, -OCH3, etc.) havepositive effect on achvity, while electron-attracting groups(such as -NO2, -Br, etc.) have negativeeffect. Sterically hindered substituents at the ortho position could dramatically reduce the activity.But none of the analogs had higher cytotoxicity than taxol.
文摘A new bisindole alkaloid, melaxillarinine, was isolated from the roots of Melodinus axillaris. Its structure was established on the basis of spectral analysis.
文摘A new stilbene diglucoside, gnetifolin K, was isolated from the lianas of Gnetum parvifolium. Its structure was established as isorhapotigenin-3,4'-di-O-beta-D-glucoside on the basis of spectral and chemical evidence.
文摘A new basic dodecapeptide BPP was isolated from the water-extract of the buckwheat pollen (BP). The sequence of BPP, which was first reported, was determined by DABITC/PITC double coupling method, and confirmed by chemical synthesis. Some analogues of BPP were also synthesized by SPPS Method. The effect of these peptides on spleen lymphocytes from mice of Kunming inbred line and on release of SIL-2R from human lymphocytes were studied. The results showed that these peptides exhibit certain immune activity.
文摘A d,I mixture (R-R and S-S) can be resolved into the d and the I enantiomers. while the mesoisomer (R-S, identical with S-R) cannot be physically separated. This fact has occasionally been 'extrapolated' to differentiate d,l and meso isomers with a chiral shift reagent where the meso isomer is presumed not to show any signal 'doubling' during the (usually) downfield shift. This assumption hasbeen shown to be incorrect by the behavior of a meso molecule which does give signal 'doubling'.
文摘A high-performance liquid chromatographic method using uv detection for thedetermination of 10 drugs of abuse is described. The method is sample and sensitive. The linear rangesare 20￣100ng/ml with relative standard deviations of 0.7 ￣ 2.6% (n=7) and detection limits of 5￣ 10ng/ml.
文摘Four new taxoids with rearranged taxane skeleton,named taxawallins C(1),D(2), F(3)and H(4),were isolated from the needles of Taxus wallichiana Zucc.. Their structures were elucidated by spectroscopic techniques.
文摘From the aerial part of Hypericum japonicum Thunb. ex Murray, two new chromone glycosides, 5, 7-Dihydroxy-2-(1-methylpropyl) chromone-8-beta-D-glucoside and 5, 7-Dihydroxy-2-isopropylchromone-8-beta-D-glucoside, were isolated and their structures were determined on basis of spectroscopic evidences.
文摘Howiinol A, an active antitumor constituent from the root and stem bark of Goniothamus howii Meer. (Annoaceae) has been synthesized in nine steps from alpha - D - glucoheptonic gamma - lactone with an overall yield of 12.4%. The absolute configuration of natural howiinol A is further confirmed.
文摘The absolute stereochemistries of Annonaceous acetogenins, tonkinin A and B, have been established by the use of the convenient conversion to their formaldehyde acetal derivatives and the subsequent application of the Mosher ester methodology.
文摘7-Epi-howiinol A, an antitumor lactone, has been synthesized in nine steps starting from alpha-D-glucoheptonic gamma-lactone with an overall yield of 8.5%.
文摘A novel bioactive Annonaceous acetogenin, donhexocin (1), has been isolated from the roots of Goniothalamus donnaiensis (Annonaceae). 1 is a new acetogenin lacking THF rings but bearing six hydroxyls including two vic-diol systems in the aliphatic chain. Its structure and stereochemistry has been elucidated by spectoscopic analysis and derivatization.
文摘A derivative of 2-arylbenzofuranoid pattern fashioned after the naturally occurring isolates from Salviamiltiorrhiza was synthesized by Perkin condensation and oxidative ring closure.
